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Sentinel lymph node mapping by near-infrared fluorescence imaging and contrast-enhanced ultrasound in healthy dogs

Sophie Favril (UGent) , Emmelie Stock (UGent) , Sophie Hernot, Myriam Hesta (UGent) , Ingeborgh Polis (UGent) , Katrien Vanderperren (UGent) and Hilde De Rooster (UGent)
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Abstract
Sentinel lymph node (SLN) mapping is a valuable and crucial diagnostic procedure in staging malignancies. We compared two non-invasive techniques, near-infrared (NIR) fluorescence imaging and contrast-enhanced ultrasound (CEUS), to identify the SLNs in three superficial anatomical regions in an animal model. Six healthy laboratory dogs were included in a proof-of-concept trial. A NIR fluorescent dye (Indocyanine Green) and microbubbles (Sonovue) were consecutively injected subdermally in the Inguinal, axillary and popliteal region to map the SLNs. Transcutaneous NIR fluorescence imaging identified SLNs in 17 out of a total of 18 occasions. CEUS identified SLNs in all regions (18/18). Whereas NIR fluorescence imaging performed better in the visualization of the afferent lymphatic tract, CEUS demonstrated different filling patterns of the SLNs, a feature potentially critical for the concept of SLN mapping in cancer patients. Both NIR fluorescence imaging and CEUS are safe, non-invasive, practical and accurate methods to perform real-time transcutaneous SLN mapping with potential in a clinical setting.
Keywords
contrast-enhanced ultrasound, dogs, indocyanine green, near-infrared fluorescence imaging, sentinel lymph node mapping, COMPUTED TOMOGRAPHIC-LYMPHOGRAPHY, METHYLENE-BLUE DYE, MAMMARY-GLANDS, BREAST-CANCER, INTRADERMAL MICROBUBBLES, INDOCYANINE GREEN, DRAINAGE, BIOPSY, IDENTIFICATION, SURGERY

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Chicago
Favril, Sophie, Emmelie Stock, Sophie Hernot, Myriam Hesta, Ingeborgh Polis, Katrien Vanderperren, and Hilde De Rooster. 2019. “Sentinel Lymph Node Mapping by Near-infrared Fluorescence Imaging and Contrast-enhanced Ultrasound in Healthy Dogs.” Veterinary and Comparative Oncology 17 (1): 89–98.
APA
Favril, S., Stock, E., Hernot, S., Hesta, M., Polis, I., Vanderperren, K., & De Rooster, H. (2019). Sentinel lymph node mapping by near-infrared fluorescence imaging and contrast-enhanced ultrasound in healthy dogs. VETERINARY AND COMPARATIVE ONCOLOGY, 17(1), 89–98.
Vancouver
1.
Favril S, Stock E, Hernot S, Hesta M, Polis I, Vanderperren K, et al. Sentinel lymph node mapping by near-infrared fluorescence imaging and contrast-enhanced ultrasound in healthy dogs. VETERINARY AND COMPARATIVE ONCOLOGY. 2019;17(1):89–98.
MLA
Favril, Sophie et al. “Sentinel Lymph Node Mapping by Near-infrared Fluorescence Imaging and Contrast-enhanced Ultrasound in Healthy Dogs.” VETERINARY AND COMPARATIVE ONCOLOGY 17.1 (2019): 89–98. Print.
@article{8587234,
  abstract     = {Sentinel lymph node (SLN) mapping is a valuable and crucial diagnostic procedure in staging malignancies. We compared two non-invasive techniques, near-infrared (NIR) fluorescence imaging and contrast-enhanced ultrasound (CEUS), to identify the SLNs in three superficial anatomical regions in an animal model. Six healthy laboratory dogs were included in a proof-of-concept trial. A NIR fluorescent dye (Indocyanine Green) and microbubbles (Sonovue) were consecutively injected subdermally in the Inguinal, axillary and popliteal region to map the SLNs. Transcutaneous NIR fluorescence imaging identified SLNs in 17 out of a total of 18 occasions. CEUS identified SLNs in all regions (18/18). Whereas NIR fluorescence imaging performed better in the visualization of the afferent lymphatic tract, CEUS demonstrated different filling patterns of the SLNs, a feature potentially critical for the concept of SLN mapping in cancer patients. Both NIR fluorescence imaging and CEUS are safe, non-invasive, practical and accurate methods to perform real-time transcutaneous SLN mapping with potential in a clinical setting.},
  author       = {Favril, Sophie and Stock, Emmelie and Hernot, Sophie and Hesta, Myriam and Polis, Ingeborgh and Vanderperren, Katrien and De Rooster, Hilde},
  issn         = {1476-5810},
  journal      = {VETERINARY AND COMPARATIVE ONCOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {89--98},
  title        = {Sentinel lymph node mapping by near-infrared fluorescence imaging and contrast-enhanced ultrasound in healthy dogs},
  url          = {http://dx.doi.org/10.1111/vco.12449},
  volume       = {17},
  year         = {2019},
}

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