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Methylome and epialleles in rice epilines selected for energy use efficiency

(2018) AGRONOMY-BASEL. 8(9).
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Abstract
Epigenetics offers important opportunities in breeding to improve the potential yield in a wide variety of crops. Starting from a pure breeder seed lot of a rice (Oryza sativa ssp. indica) inbred population, repeated testing for improved cellular respiration rates and energy use efficiency (EUE) over three generations identified performant epilines with distinct epigenetic signatures and with improved seed yield in field trials. Epiline DNA methylomes were characterized by genome-wide bisulfite sequencing to discern cytosine methylation changes in relation to transcriptome and phenotype. Regional methylation changes were dispersed over the epiline genomes. A number of upstream-associated differentially methylated regions (DMRs) correlated with differentially expressed genes (DEGs) with a role in particular molecular functions like transmembrane transport and protein kinase activity. Targeted bisulfite sequencing confirmed epiline DMRs that anti-correlated with DEGs, identifying putative epialleles that were susceptible for cytosine methylation changes that might affect gene expression and contribute to the phenotype. Chromatin immunoprecipitation sequencing revealed the extensive enrichment of gene-associated histone H3 lysine-4 trimethylation (H3K4me3), which correlated with gene activation and reduced cytosine methylation. Our data indicate that seed formation is prone to epigenetic changes that might be used as a resource in crop improvement.
Keywords
CYCLIC ELECTRON FLOW, PENTATRICOPEPTIDE REPEAT PROTEINS, DNA, METHYLATION, PHOTOSYSTEM-I, EPIGENETIC INHERITANCE, CYTOSINE, METHYLATION, HISTONE METHYLATION, PHYSIOLOGICAL-ROLE, COMPLEX PERFORMS, GENE-EXPRESSION, transcriptome, cytosine methylation, post-translational histone H3, modification, cellular respiration, growth, seed yield

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Chicago
Schmidt, Martin, Marina Byzova, Cindy Martens, Marrit Peeters, Yog Raj, Shailesh Shukla, Tom Verwulgen, Marc De Block, and Maria Van Lijsebettens. 2018. “Methylome and Epialleles in Rice Epilines Selected for Energy Use Efficiency.” Agronomy-basel 8 (9).
APA
Schmidt, Martin, Byzova, M., Martens, C., Peeters, M., Raj, Y., Shukla, S., Verwulgen, T., et al. (2018). Methylome and epialleles in rice epilines selected for energy use efficiency. AGRONOMY-BASEL, 8(9).
Vancouver
1.
Schmidt M, Byzova M, Martens C, Peeters M, Raj Y, Shukla S, et al. Methylome and epialleles in rice epilines selected for energy use efficiency. AGRONOMY-BASEL. 2018;8(9).
MLA
Schmidt, Martin et al. “Methylome and Epialleles in Rice Epilines Selected for Energy Use Efficiency.” AGRONOMY-BASEL 8.9 (2018): n. pag. Print.
@article{8586139,
  abstract     = {Epigenetics offers important opportunities in breeding to improve the potential yield in a wide variety of crops. Starting from a pure breeder seed lot of a rice (Oryza sativa ssp. indica) inbred population, repeated testing for improved cellular respiration rates and energy use efficiency (EUE) over three generations identified performant epilines with distinct epigenetic signatures and with improved seed yield in field trials. Epiline DNA methylomes were characterized by genome-wide bisulfite sequencing to discern cytosine methylation changes in relation to transcriptome and phenotype. Regional methylation changes were dispersed over the epiline genomes. A number of upstream-associated differentially methylated regions (DMRs) correlated with differentially expressed genes (DEGs) with a role in particular molecular functions like transmembrane transport and protein kinase activity. Targeted bisulfite sequencing confirmed epiline DMRs that anti-correlated with DEGs, identifying putative epialleles that were susceptible for cytosine methylation changes that might affect gene expression and contribute to the phenotype. Chromatin immunoprecipitation sequencing revealed the extensive enrichment of gene-associated histone H3 lysine-4 trimethylation (H3K4me3), which correlated with gene activation and reduced cytosine methylation. Our data indicate that seed formation is prone to epigenetic changes that might be used as a resource in crop improvement.},
  articleno    = {163},
  author       = {Schmidt, Martin and Byzova, Marina and Martens, Cindy and Peeters, Marrit and Raj, Yog and Shukla, Shailesh and Verwulgen, Tom and De Block, Marc and Van Lijsebettens, Maria},
  issn         = {2073-4395},
  journal      = {AGRONOMY-BASEL},
  language     = {eng},
  number       = {9},
  pages        = {21},
  title        = {Methylome and epialleles in rice epilines selected for energy use efficiency},
  url          = {http://dx.doi.org/10.3390/agronomy8090163},
  volume       = {8},
  year         = {2018},
}

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