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In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter

(2018) PLOS ONE. 13(9).
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Abstract
Campylobacteriosis is a widespread infectious disease, leading to a major health and economic burden. Chickens are considered as the most common infection source for humans. Campylobacter mainly multiplies in the mucus layer of their caeca. No effective control measures are currently available, but passive immunisation of chickens with pathogen-specific maternal IgY antibodies, present in egg yolk of immunised chickens, reduces Campylobacter colonisation. To explore this strategy further, anti-Campylobacter nanobodies, directed against the flagella and major outer membrane proteins, were fused to the constant domains of chicken IgA and IgY, combining the benefits of nanobodies and the effector functions of the Fc-domains. The designer chimeric antibodies were effectively produced in leaves of Nicotiana benthamiana and seeds of Arabidopsis thaliana. Stable expression of the chimeric antibodies in seeds resulted in production levels between 1% and 8% of the total soluble protein. These in planta produced antibodies do not only bind to their purified antigens but also to Campylobacter bacterial cells. In addition, the anti-flagellin chimeric antibodies are reducing the motility of Campylobacter bacteria. These antibody-containing Arabidopsis seeds can be tested for oral passive immunisation of chickens and, if effective, the chimeric antibodies can be produced in crop seeds.
Keywords
ESCHERICHIA-COLI INFECTION, OUTER-MEMBRANE PROTEIN, RECOMBINANT ANTIBODIES, TRANSIENT EXPRESSION, JEJUNI COLONIZATION, ARABIDOPSIS SEEDS, PASSIVE-IMMUNITY, AGROBACTERIUM, CHAIN, SYSTEMS

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Chicago
Vanmarsenille, Charlotte, Jelle Elseviers, Charlotte Yvanoff, Gholamreza Hassanzadeh-Ghassabeh, Gabriela Garcia Rodriguez, Edo Martens, Anna Depicker, et al. 2018. “In Planta Expression of Nanobody-based Designer Chicken Antibodies Targeting Campylobacter.” Plos One 13 (9).
APA
Vanmarsenille, C., Elseviers, J., Yvanoff, C., Hassanzadeh-Ghassabeh, G., Garcia Rodriguez, G., Martens, E., Depicker, A., et al. (2018). In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter. PLOS ONE, 13(9).
Vancouver
1.
Vanmarsenille C, Elseviers J, Yvanoff C, Hassanzadeh-Ghassabeh G, Garcia Rodriguez G, Martens E, et al. In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter. PLOS ONE. 2018;13(9).
MLA
Vanmarsenille, Charlotte, Jelle Elseviers, Charlotte Yvanoff, et al. “In Planta Expression of Nanobody-based Designer Chicken Antibodies Targeting Campylobacter.” PLOS ONE 13.9 (2018): n. pag. Print.
@article{8586136,
  abstract     = {Campylobacteriosis is a widespread infectious disease, leading to a major health and economic burden. Chickens are considered as the most common infection source for humans. Campylobacter mainly multiplies in the mucus layer of their caeca. No effective control measures are currently available, but passive immunisation of chickens with pathogen-specific maternal IgY antibodies, present in egg yolk of immunised chickens, reduces Campylobacter colonisation. To explore this strategy further, anti-Campylobacter nanobodies, directed against the flagella and major outer membrane proteins, were fused to the constant domains of chicken IgA and IgY, combining the benefits of nanobodies and the effector functions of the Fc-domains. The designer chimeric antibodies were effectively produced in leaves of Nicotiana benthamiana and seeds of Arabidopsis thaliana. Stable expression of the chimeric antibodies in seeds resulted in production levels between 1\% and 8\% of the total soluble protein. These in planta produced antibodies do not only bind to their purified antigens but also to Campylobacter bacterial cells. In addition, the anti-flagellin chimeric antibodies are reducing the motility of Campylobacter bacteria. These antibody-containing Arabidopsis seeds can be tested for oral passive immunisation of chickens and, if effective, the chimeric antibodies can be produced in crop seeds.},
  articleno    = {e0204222},
  author       = {Vanmarsenille, Charlotte and Elseviers, Jelle and Yvanoff, Charlotte and Hassanzadeh-Ghassabeh, Gholamreza and Garcia Rodriguez, Gabriela and Martens, Edo and Depicker, Anna and Martel, An and Haesebrouck, Freddy and Pasmans, Frank and Hernalsteens, Jean-Pierre and De Greve, Henri},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {9},
  pages        = {23},
  title        = {In planta expression of nanobody-based designer chicken antibodies targeting Campylobacter},
  url          = {http://dx.doi.org/10.1371/journal.pone.0204222},
  volume       = {13},
  year         = {2018},
}

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