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MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems

(2018) PLANT JOURNAL. 96(5). p.895-909
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Abstract
The Mediator (MED) complex plays a key role in the recruitment and assembly of the transcription machinery for the control of gene expression. Here, we report on the role of MEDIATOR18 (MED18) subunit in root development, auxin signaling and meristem cell viability in Arabidopsis thaliana seedlings. Loss-of-function mutations in MED18 reduce primary root growth, but increase lateral root formation and root hair development. This phenotype correlates with alterations in cell division and elongation likely caused by an increased auxin response and transport at the root tip, as evidenced by DR5:GFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP and pPIN3::PIN3-GFP auxin-related gene expression. Noteworthy, med18 seedlings manifest cell death in the root meristem, which exacerbates with age and/or exposition to DNA-damaging agents, and display high expression of the cell regeneration factor ERF115. Cell death in the root tip was reduced in med18 seedlings grown in darkness, but remained when only the shoot was exposed to light, suggesting that MED18 acts to protect root meristem cells from local cell death, and/or in response to root-acting signal(s) emitted by the shoot in response to light stimuli. These data point to MED18 as an important component for auxin-regulated root development, cell death and cell regeneration in root meristems.
Keywords
Arabidopsis thaliana, root development, auxin signaling, cell death, regeneration, DNA-DAMAGE RESPONSES, GENE-EXPRESSION, HOMOLOGOUS RECOMBINATION, ACTS, MAINTENANCE, SUBUNIT, REPAIR, DIFFERENTIATION, AVAILABILITY, MECHANISMS

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Chicago
Raya-Gonzalez, Javier, Araceli Oropeza-Aburto, Jesus S Lopez-Bucio, Angel A Guevara-Garcia, Lieven De Veylder, Jose Lopez-Bucio, and Luis Herrera-Estrella. 2018. “MEDIATOR18 Influences Arabidopsis Root Architecture, Represses Auxin Signaling and Is a Critical Factor for Cell Viability in Root Meristems.” Plant Journal 96 (5): 895–909.
APA
Raya-Gonzalez, J., Oropeza-Aburto, A., Lopez-Bucio, J. S., Guevara-Garcia, A. A., De Veylder, L., Lopez-Bucio, J., & Herrera-Estrella, L. (2018). MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems. PLANT JOURNAL, 96(5), 895–909.
Vancouver
1.
Raya-Gonzalez J, Oropeza-Aburto A, Lopez-Bucio JS, Guevara-Garcia AA, De Veylder L, Lopez-Bucio J, et al. MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems. PLANT JOURNAL. 2018;96(5):895–909.
MLA
Raya-Gonzalez, Javier et al. “MEDIATOR18 Influences Arabidopsis Root Architecture, Represses Auxin Signaling and Is a Critical Factor for Cell Viability in Root Meristems.” PLANT JOURNAL 96.5 (2018): 895–909. Print.
@article{8586134,
  abstract     = {The Mediator (MED) complex plays a key role in the recruitment and assembly of the transcription machinery for the control of gene expression. Here, we report on the role of MEDIATOR18 (MED18) subunit in root development, auxin signaling and meristem cell viability in Arabidopsis thaliana seedlings. Loss-of-function mutations in MED18 reduce primary root growth, but increase lateral root formation and root hair development. This phenotype correlates with alterations in cell division and elongation likely caused by an increased auxin response and transport at the root tip, as evidenced by DR5:GFP, pPIN1::PIN1-GFP, pPIN2::PIN2-GFP and pPIN3::PIN3-GFP auxin-related gene expression. Noteworthy, med18 seedlings manifest cell death in the root meristem, which exacerbates with age and/or exposition to DNA-damaging agents, and display high expression of the cell regeneration factor ERF115. Cell death in the root tip was reduced in med18 seedlings grown in darkness, but remained when only the shoot was exposed to light, suggesting that MED18 acts to protect root meristem cells from local cell death, and/or in response to root-acting signal(s) emitted by the shoot in response to light stimuli. These data point to MED18 as an important component for auxin-regulated root development, cell death and cell regeneration in root meristems.},
  author       = {Raya-Gonzalez, Javier and Oropeza-Aburto, Araceli and Lopez-Bucio, Jesus S and Guevara-Garcia, Angel A and De Veylder, Lieven and Lopez-Bucio, Jose and Herrera-Estrella, Luis},
  issn         = {0960-7412},
  journal      = {PLANT JOURNAL},
  language     = {eng},
  number       = {5},
  pages        = {895--909},
  title        = {MEDIATOR18 influences Arabidopsis root architecture, represses auxin signaling and is a critical factor for cell viability in root meristems},
  url          = {http://dx.doi.org/10.1111/tpj.14114},
  volume       = {96},
  year         = {2018},
}

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