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POLAR-guided signalling complex assembly and localization drive asymmetric cell division

(2018) NATURE. 563(7732). p.574-578
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Abstract
Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival(1-4). In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module(5,6) and its downstream target, the transcription factor SPEECHLESS (SPCH) 7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.
Keywords
ARABIDOPSIS-THALIANA, STOMATAL DEVELOPMENT, GSK3-LIKE KINASES, FATE, PHOSPHORYLATION, INHIBITION, DYNAMICS

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MLA
Houbaert, Anaxi, Cheng Zhang, Manish Tiwari, et al. “POLAR-guided Signalling Complex Assembly and Localization Drive Asymmetric Cell Division.” NATURE 563.7732 (2018): 574–578. Print.
APA
Houbaert, A., Zhang, C., Tiwari, M., Wang, K., Serrano, A. de M., Savatin, D., Urs, M. J., et al. (2018). POLAR-guided signalling complex assembly and localization drive asymmetric cell division. NATURE, 563(7732), 574–578.
Chicago author-date
Houbaert, Anaxi, Cheng Zhang, Manish Tiwari, Kun Wang, Alberto de Marcos Serrano, Daniel Savatin, Mounashree J Urs, et al. 2018. “POLAR-guided Signalling Complex Assembly and Localization Drive Asymmetric Cell Division.” Nature 563 (7732): 574–578.
Chicago author-date (all authors)
Houbaert, Anaxi, Cheng Zhang, Manish Tiwari, Kun Wang, Alberto de Marcos Serrano, Daniel Savatin, Mounashree J Urs, Miroslava Zhiponova, Gustavo Gudesblat, Isabelle Vanhoutte, Dominique Eeckhout, Sjef Boeren, Mansour Karimi, Camilla Betti, Thomas Jacobs, Carmen Fenoll, Montana Mena, Sacco de Vries, Geert De Jaeger, and Eugenia Russinova. 2018. “POLAR-guided Signalling Complex Assembly and Localization Drive Asymmetric Cell Division.” Nature 563 (7732): 574–578.
Vancouver
1.
Houbaert A, Zhang C, Tiwari M, Wang K, Serrano A de M, Savatin D, et al. POLAR-guided signalling complex assembly and localization drive asymmetric cell division. NATURE. 2018;563(7732):574–8.
IEEE
[1]
A. Houbaert et al., “POLAR-guided signalling complex assembly and localization drive asymmetric cell division,” NATURE, vol. 563, no. 7732, pp. 574–578, 2018.
@article{8586086,
  abstract     = {Stomatal cell lineage is an archetypal example of asymmetric cell division (ACD), which is necessary for plant survival(1-4). In Arabidopsis thaliana, the GLYCOGEN SYNTHASE KINASE3 (GSK3)/SHAGGY-like kinase BRASSINOSTEROID INSENSITIVE 2 (BIN2) phosphorylates both the mitogen-activated protein kinase (MAPK) signalling module(5,6) and its downstream target, the transcription factor SPEECHLESS (SPCH) 7, to promote and restrict ACDs, respectively, in the same stomatal lineage cell. However, the mechanisms that balance these mutually exclusive activities remain unclear. Here we identify the plant-specific protein POLAR as a stomatal lineage scaffold for a subset of GSK3-like kinases that confines them to the cytosol and subsequently transiently polarizes them within the cell, together with BREAKING OF ASYMMETRY IN THE STOMATAL LINEAGE (BASL), before ACD. As a result, MAPK signalling is attenuated, enabling SPCH to drive ACD in the nucleus. Moreover, POLAR turnover requires phosphorylation on specific residues, mediated by GSK3. Our study reveals a mechanism by which the scaffolding protein POLAR ensures GSK3 substrate specificity, and could serve as a paradigm for understanding regulation of GSK3 in plants.},
  author       = {Houbaert, Anaxi and Zhang, Cheng and Tiwari, Manish and Wang, Kun and Serrano, Alberto de Marcos and Savatin, Daniel and Urs, Mounashree J and Zhiponova, Miroslava and Gudesblat, Gustavo and Vanhoutte, Isabelle and Eeckhout, Dominique and Boeren, Sjef and Karimi, Mansour and Betti, Camilla and Jacobs, Thomas and Fenoll, Carmen and Mena, Montana and de Vries, Sacco and De Jaeger, Geert and Russinova, Eugenia},
  issn         = {0028-0836},
  journal      = {NATURE},
  keywords     = {ARABIDOPSIS-THALIANA,STOMATAL DEVELOPMENT,GSK3-LIKE KINASES,FATE,PHOSPHORYLATION,INHIBITION,DYNAMICS},
  language     = {eng},
  number       = {7732},
  pages        = {574--578},
  title        = {POLAR-guided signalling complex assembly and localization drive asymmetric cell division},
  url          = {http://dx.doi.org/10.1038/s41586-018-0714-x},
  volume       = {563},
  year         = {2018},
}

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