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Aronia (Aronia melanocarpa) polyphenols modulate the microbial community in a simulator of the human intestinal microbial ecosystem (SHIME) and decrease secretion of proinflammatory markers in a Caco-2/endothelial cell coculture model

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Abstract
Scope: To explore the mechanisms behind the health effects of Aronia (Aronia melanocarpa), the microbial community modulating and anti-inflammatory effects of Aronia polyphenols are investigated by combining the similutor of the human intestinal microbial ecosystem (SHIME) with a coculture of intestinal and endothelial cells. Results: Administration of Aronia juice (6.5g L-1) to the SHIME for 2 weeks increases the abundance of firmicutes to 92% in the ascending colon (AC), 85% in the transverse colon (TC), and 82% in the descending colon (DC; p < 0.001), proteobacteria (6.7% in AC, p < 0.001), and Akkermansia (14% in TC and 18% in DC, p < 0.001) and decreases the abundance of Bifidobacterium species, associated with a decrease of acetate and increase of propionate and butyrate, whereas no significant difference is observed upon placebo juice treatment. After addition of the digests to TNF-alpha challenged Caco-2/endothelial cocultures, intercellular adhesion molecule (ICAM)-1, IL-8, and monocyte chemoattractant protein-1 levels are significantly downregulated. Interestingly, Aronia juice treats digests from each colon compartment resulting in a stronger decrease of the ICAM-1 secretion (up to 73%, p < 0.001) compared to their corresponding placebo treated digests, thereby pointing to a polyphenol-dependent effect. Conclusions: Aronia polyphenols modulate intestinal microbial composition, induce beneficial short chain fatty acid production, and prevent inflammatory stress in endothelial cells. This opens perspectives for the use of Aronia polyphenols as prebiotics in the context of intestinal and cardiovascular health.
Keywords
cocultures, in vitro digestion, inflammation, microbiota, polyphenols, LOW-GRADE INFLAMMATION, IN-VITRO, AKKERMANSIA-MUCINIPHILA, DIETARY POLYPHENOLS, COLONIC MICROBIOTA, ENDOTHELIAL-CELLS, BLOOD-PRESSURE, TNF-ALPHA, BIOAVAILABILITY, CONSUMPTION

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Chicago
Wu, Ting, Charlotte Grootaert, Judit Pitart, Nevena Kardum Vidovic, Senem Kamiloglu, Sam Possemiers, Maria Glibetic, et al. 2018. “Aronia (Aronia Melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model.” Molecular Nutrition & Food Research 62 (22).
APA
Wu, Ting, Grootaert, C., Pitart, J., Vidovic, N. K., Kamiloglu, S., Possemiers, S., Glibetic, M., et al. (2018). Aronia (Aronia melanocarpa) polyphenols modulate the microbial community in a simulator of the human intestinal microbial ecosystem (SHIME) and decrease secretion of proinflammatory markers in a Caco-2/endothelial cell coculture model. MOLECULAR NUTRITION & FOOD RESEARCH, 62(22).
Vancouver
1.
Wu T, Grootaert C, Pitart J, Vidovic NK, Kamiloglu S, Possemiers S, et al. Aronia (Aronia melanocarpa) polyphenols modulate the microbial community in a simulator of the human intestinal microbial ecosystem (SHIME) and decrease secretion of proinflammatory markers in a Caco-2/endothelial cell coculture model. MOLECULAR NUTRITION & FOOD RESEARCH. 2018;62(22).
MLA
Wu, Ting, Charlotte Grootaert, Judit Pitart, et al. “Aronia (Aronia Melanocarpa) Polyphenols Modulate the Microbial Community in a Simulator of the Human Intestinal Microbial Ecosystem (SHIME) and Decrease Secretion of Proinflammatory Markers in a Caco-2/endothelial Cell Coculture Model.” MOLECULAR NUTRITION & FOOD RESEARCH 62.22 (2018): n. pag. Print.
@article{8585940,
  abstract     = {Scope: To explore the mechanisms behind the health effects of Aronia (Aronia melanocarpa), the microbial community modulating and anti-inflammatory effects of Aronia polyphenols are investigated by combining the similutor of the human intestinal microbial ecosystem (SHIME) with a coculture of intestinal and endothelial cells.
Results: Administration of Aronia juice (6.5g L-1) to the SHIME for 2 weeks increases the abundance of firmicutes to 92\% in the ascending colon (AC), 85\% in the transverse colon (TC), and 82\% in the descending colon (DC; p {\textlangle} 0.001), proteobacteria (6.7\% in AC, p {\textlangle} 0.001), and Akkermansia (14\% in TC and 18\% in DC, p {\textlangle} 0.001) and decreases the abundance of Bifidobacterium species, associated with a decrease of acetate and increase of propionate and butyrate, whereas no significant difference is observed upon placebo juice treatment. After addition of the digests to TNF-alpha challenged Caco-2/endothelial cocultures, intercellular adhesion molecule (ICAM)-1, IL-8, and monocyte chemoattractant protein-1 levels are significantly downregulated. Interestingly, Aronia juice treats digests from each colon compartment resulting in a stronger decrease of the ICAM-1 secretion (up to 73\%, p {\textlangle} 0.001) compared to their corresponding placebo treated digests, thereby pointing to a polyphenol-dependent effect.
Conclusions: Aronia polyphenols modulate intestinal microbial composition, induce beneficial short chain fatty acid production, and prevent inflammatory stress in endothelial cells. This opens perspectives for the use of Aronia polyphenols as prebiotics in the context of intestinal and cardiovascular health.},
  articleno    = {1800607},
  author       = {Wu, Ting and Grootaert, Charlotte and Pitart, Judit and Vidovic, Nevena Kardum and Kamiloglu, Senem and Possemiers, Sam and Glibetic, Maria and Smagghe, Guy and Raes, Katleen and Van de Wiele, Tom and Van Camp, John},
  issn         = {1613-4125},
  journal      = {MOLECULAR NUTRITION \& FOOD RESEARCH},
  language     = {eng},
  number       = {22},
  pages        = {10},
  title        = {Aronia (Aronia melanocarpa) polyphenols modulate the microbial community in a simulator of the human intestinal microbial ecosystem (SHIME) and decrease secretion of proinflammatory markers in a Caco-2/endothelial cell coculture model},
  url          = {http://dx.doi.org/10.1002/mnfr.201800607},
  volume       = {62},
  year         = {2018},
}

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