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Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa

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Abstract
The ability of bacteria to infect a host relies in part on the secretion of molecular virulence factors across the cell envelope.Pseudomonas aeruginosa, a ubiquitous environmental bacterium causing opportunistic infections in humans, employs the type II secretion system (T2SS) to transport effector proteins across its cellular envelope as part of a diverse array of virulence strategies.General secretory pathway protein L (GspL) is an essential inner-membrane component of the T2SS apparatus, and is thought to facilitate transduction of the energy from ATP hydrolysis in the cytoplasm to the periplasmic components of the system.However,our incomplete understanding of the assembly principles of the T2SS machinery prevents the mechanistic deconvolution of T2SS-mediated protein secretion.Here we show via two crystal structures that the periplasmic ferredoxin-like domain of GspL (GspL(fld)) is a dimer stabilized by hydrophobic interactions, and that this interface may allow significant interdomain plasticity.The general dimerization mode of GspL(fld) is shared with GspL from Vibrio parahaemolyticus suggesting a conserved oligomerization mode across the GspL family. Furthermore, we identified a tetrameric form of the complete periplasmic segment of GspL (GspL(Peri)) which indicates that GspL may be able to adopt multiple oligomeric states as part of its dynamic role in the T2SS apparatus.
Keywords
VIBRIO-CHOLERAE, CYTOPLASMIC DOMAIN, PROTEIN SECRETION, CRYSTAL-STRUCTURE, NONCRYSTALLOGRAPHIC SYMMETRY, PILO FORM, IN-VIVO, INSIGHTS, MEMBRANE, EPSL

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Chicago
Fulara, Aleksandra, Isabel Vandenberghe, Randy J Read, Bart Devreese, and Savvas Savvides. 2018. “Structure and Oligomerization of the Periplasmic Domain of GspL from the Type II Secretion System of Pseudomonas Aeruginosa.” Scientific Reports 8.
APA
Fulara, A., Vandenberghe, I., Read, R. J., Devreese, B., & Savvides, S. (2018). Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa. SCIENTIFIC REPORTS, 8.
Vancouver
1.
Fulara A, Vandenberghe I, Read RJ, Devreese B, Savvides S. Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa. SCIENTIFIC REPORTS. 2018;8.
MLA
Fulara, Aleksandra et al. “Structure and Oligomerization of the Periplasmic Domain of GspL from the Type II Secretion System of Pseudomonas Aeruginosa.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
@article{8585917,
  abstract     = {The ability of bacteria to infect a host relies in part on the secretion of molecular virulence factors across the cell envelope.Pseudomonas aeruginosa, a ubiquitous environmental bacterium causing opportunistic infections in humans, employs the type II secretion system (T2SS) to transport effector proteins across its cellular envelope as part of a diverse array of virulence strategies.General secretory pathway protein L (GspL) is an essential inner-membrane component of the T2SS apparatus, and is thought to facilitate transduction of the energy from ATP hydrolysis in the cytoplasm to the periplasmic components of the system.However,our incomplete understanding of the assembly principles of the T2SS machinery prevents the mechanistic deconvolution of T2SS-mediated protein secretion.Here we show via two crystal structures that the periplasmic ferredoxin-like domain of GspL (GspL(fld)) is a dimer stabilized by hydrophobic interactions, and that this interface may allow significant interdomain plasticity.The general dimerization mode of GspL(fld) is shared with GspL from Vibrio parahaemolyticus suggesting a conserved oligomerization mode across the GspL family. Furthermore, we identified a tetrameric form of the complete periplasmic segment of GspL (GspL(Peri)) which indicates that GspL may be able to adopt multiple oligomeric states as part of its dynamic role in the T2SS apparatus.},
  articleno    = {16760},
  author       = {Fulara, Aleksandra and Vandenberghe, Isabel and Read, Randy J and Devreese, Bart and Savvides, Savvas},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  language     = {eng},
  pages        = {14},
  title        = {Structure and oligomerization of the periplasmic domain of GspL from the type II secretion system of Pseudomonas aeruginosa},
  url          = {http://dx.doi.org/10.1038/s41598-018-34956-w},
  volume       = {8},
  year         = {2018},
}

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