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Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid

Lisa Delahaye (UGent) , Evelyn Dhont (UGent) , Pieter De Cock (UGent) , Peter De Paepe (UGent) and Christophe Stove (UGent)
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Abstract
In the field of bioanalysis, dried matrix spot sampling is increasingly receiving interest, as this alternative sampling strategy offers many potential benefits over traditional sampling, including matrix volume-sparing properties. By using a microsampling strategy, e.g., volumetric absorptive microsampling (VAMS), the number of samples that can be collected from a patient can be increased, as a result of the limited sample volume that is required per sample. To date, no VAMS-based methods have been developed for the quantification of analytes in cerebrospinal fluid (CSF). The objective of this study was to develop and validate two LC-MS/MS methods for the quantification of paracetamol in dried blood and dried CSF, with both matrices sampled using VAMS. Both methods were fully validated based on internationally accepted guidelines. Paracetamol was chromatographically separated from its glucuronide and sulfate metabolites and no carry-over or unacceptable interferences were detected. The total precision (%RSD) was below 15% for all QC levels and accuracy (%bias) was below 7% (17% for the LLOQ of aqueous VAMS). The influence of the hematocrit on the recovery of blood VAMS samples appeared to be limited within the hematocrit range of 0.21 to 0.62. The blood VAMS samples were stable for 1week if stored at 50 degrees C, and for at least 8months when stored between -80 degrees C and room temperature. The aqueous VAMS samples were stable for at least 9months when stored between -80 and 4 degrees C, and for 1month when stored at room temperature. Application of the methods on external quality control material and analysis of patient samples demonstrated the validity and utility of the methods and provided a proof of concept for the analysis of CSF microsamples obtained via VAMS devices.
Keywords
Volumetric absorptive microsampling, Liquid chromatography-tandem mass spectrometry, Alternative sampling strategies, Cerebrospinal fluid, Paracetamol, QUANTITATIVE BIOANALYSIS, DRUG, STANDARD, ISSUES, SPOTS, BIAS

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MLA
Delahaye, Lisa, Evelyn Dhont, Pieter De Cock, et al. “Volumetric Absorptive Microsampling as an Alternative Sampling Strategy for the Determination of Paracetamol in Blood and Cerebrospinal Fluid.” ANALYTICAL AND BIOANALYTICAL CHEMISTRY 411.1 (2019): 181–191. Print.
APA
Delahaye, L., Dhont, E., De Cock, P., De Paepe, P., & Stove, C. (2019). Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid. ANALYTICAL AND BIOANALYTICAL CHEMISTRY, 411(1), 181–191.
Chicago author-date
Delahaye, Lisa, Evelyn Dhont, Pieter De Cock, Peter De Paepe, and Christophe Stove. 2019. “Volumetric Absorptive Microsampling as an Alternative Sampling Strategy for the Determination of Paracetamol in Blood and Cerebrospinal Fluid.” Analytical and Bioanalytical Chemistry 411 (1): 181–191.
Chicago author-date (all authors)
Delahaye, Lisa, Evelyn Dhont, Pieter De Cock, Peter De Paepe, and Christophe Stove. 2019. “Volumetric Absorptive Microsampling as an Alternative Sampling Strategy for the Determination of Paracetamol in Blood and Cerebrospinal Fluid.” Analytical and Bioanalytical Chemistry 411 (1): 181–191.
Vancouver
1.
Delahaye L, Dhont E, De Cock P, De Paepe P, Stove C. Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid. ANALYTICAL AND BIOANALYTICAL CHEMISTRY. 2019;411(1):181–91.
IEEE
[1]
L. Delahaye, E. Dhont, P. De Cock, P. De Paepe, and C. Stove, “Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid,” ANALYTICAL AND BIOANALYTICAL CHEMISTRY, vol. 411, no. 1, pp. 181–191, 2019.
@article{8585807,
  abstract     = {In the field of bioanalysis, dried matrix spot sampling is increasingly receiving interest, as this alternative sampling strategy offers many potential benefits over traditional sampling, including matrix volume-sparing properties. By using a microsampling strategy, e.g., volumetric absorptive microsampling (VAMS), the number of samples that can be collected from a patient can be increased, as a result of the limited sample volume that is required per sample. To date, no VAMS-based methods have been developed for the quantification of analytes in cerebrospinal fluid (CSF). The objective of this study was to develop and validate two LC-MS/MS methods for the quantification of paracetamol in dried blood and dried CSF, with both matrices sampled using VAMS. Both methods were fully validated based on internationally accepted guidelines. Paracetamol was chromatographically separated from its glucuronide and sulfate metabolites and no carry-over or unacceptable interferences were detected. The total precision (%RSD) was below 15% for all QC levels and accuracy (%bias) was below 7% (17% for the LLOQ of aqueous VAMS). The influence of the hematocrit on the recovery of blood VAMS samples appeared to be limited within the hematocrit range of 0.21 to 0.62. The blood VAMS samples were stable for 1week if stored at 50 degrees C, and for at least 8months when stored between -80 degrees C and room temperature. The aqueous VAMS samples were stable for at least 9months when stored between -80 and 4 degrees C, and for 1month when stored at room temperature. Application of the methods on external quality control material and analysis of patient samples demonstrated the validity and utility of the methods and provided a proof of concept for the analysis of CSF microsamples obtained via VAMS devices.},
  author       = {Delahaye, Lisa and Dhont, Evelyn and De Cock, Pieter and De Paepe, Peter and Stove, Christophe},
  issn         = {1618-2642},
  journal      = {ANALYTICAL AND BIOANALYTICAL CHEMISTRY},
  keywords     = {Volumetric absorptive microsampling,Liquid chromatography-tandem mass spectrometry,Alternative sampling strategies,Cerebrospinal fluid,Paracetamol,QUANTITATIVE BIOANALYSIS,DRUG,STANDARD,ISSUES,SPOTS,BIAS},
  language     = {eng},
  number       = {1},
  pages        = {181--191},
  title        = {Volumetric absorptive microsampling as an alternative sampling strategy for the determination of paracetamol in blood and cerebrospinal fluid},
  url          = {http://dx.doi.org/10.1007/s00216-018-1427-6},
  volume       = {411},
  year         = {2019},
}

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