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Development of a host-microbiome model of the small intestine

(2019) FASEB JOURNAL. 33(3). p.3985-3996
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Abstract
The intestinal epithelium plays an essential role in the balance between tolerant and protective immune responses to infectious agents. In vitro models do not typically consider the innate immune response and gut microbiome in detail, so these models do not fully mimic the physiologic aspects of the small intestine. We developed and characterized a long-term in vitro model containing enterocyte, goblet, and immune-like cells exposed to a synthetic microbial community representative of commensal inhabitants of the small intestine. This model showed differential responses toward a synthetic microbial community of commensal bacterial inhabitants of the small intestine in the absence or presence of LPS from Escherichia coli O111:B4. Simultaneous exposure to LPS and microbiota induced impaired epithelial barrier function; increased production of IL-8, IL-6, TNF-, and C-X-C motif chemokine ligand 16; and augmented differentiation and adhesion of macrophage-like cells and the overexpression of dual oxidase 2 and TLR-2 and -4 mRNA. In addition, the model demonstrated the ability to assess the adhesion of specific bacterial strains from the synthetic microbial communitymore specifically, Veillonella parvulato the simulated epithelium. This novel in vitro model may assist in overcoming sampling and retrieval difficulties when studying host-microbiome interactions in the small intestine.
Keywords
in vitro microbiome, inflammation, Caco-2, HT29-MTX, THP-1, IN-VITRO, EPITHELIAL-CELLS, GENE-EXPRESSION, DENDRITIC CELLS, PARACELLULAR PERMEABILITY, CHEMOKINE CXCL16, BARRIER FUNCTION, COCULTURE MODEL, GUT, CACO-2

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Citation

Please use this url to cite or link to this publication:

MLA
Calatayud Arroyo, Marta et al. “Development of a Host-microbiome Model of the Small Intestine.” FASEB JOURNAL 33.3 (2019): 3985–3996. Print.
APA
Calatayud Arroyo, M., Dezutter, O., Hernandez Sanabria, E., Hidalgo Martinez, S., Meysman, F., & Van de Wiele, T. (2019). Development of a host-microbiome model of the small intestine. FASEB JOURNAL, 33(3), 3985–3996.
Chicago author-date
Calatayud Arroyo, Marta, Olivier Dezutter, Emma Hernandez Sanabria, Silvia Hidalgo Martinez, Filip Meysman, and Tom Van de Wiele. 2019. “Development of a Host-microbiome Model of the Small Intestine.” Faseb Journal 33 (3): 3985–3996.
Chicago author-date (all authors)
Calatayud Arroyo, Marta, Olivier Dezutter, Emma Hernandez Sanabria, Silvia Hidalgo Martinez, Filip Meysman, and Tom Van de Wiele. 2019. “Development of a Host-microbiome Model of the Small Intestine.” Faseb Journal 33 (3): 3985–3996.
Vancouver
1.
Calatayud Arroyo M, Dezutter O, Hernandez Sanabria E, Hidalgo Martinez S, Meysman F, Van de Wiele T. Development of a host-microbiome model of the small intestine. FASEB JOURNAL. 2019;33(3):3985–96.
IEEE
[1]
M. Calatayud Arroyo, O. Dezutter, E. Hernandez Sanabria, S. Hidalgo Martinez, F. Meysman, and T. Van de Wiele, “Development of a host-microbiome model of the small intestine,” FASEB JOURNAL, vol. 33, no. 3, pp. 3985–3996, 2019.
@article{8585292,
  abstract     = {The intestinal epithelium plays an essential role in the balance between tolerant and protective immune responses to infectious agents. In vitro models do not typically consider the innate immune response and gut microbiome in detail, so these models do not fully mimic the physiologic aspects of the small intestine. We developed and characterized a long-term in vitro model containing enterocyte, goblet, and immune-like cells exposed to a synthetic microbial community representative of commensal inhabitants of the small intestine. This model showed differential responses toward a synthetic microbial community of commensal bacterial inhabitants of the small intestine in the absence or presence of LPS from Escherichia coli O111:B4. Simultaneous exposure to LPS and microbiota induced impaired epithelial barrier function; increased production of IL-8, IL-6, TNF-, and C-X-C motif chemokine ligand 16; and augmented differentiation and adhesion of macrophage-like cells and the overexpression of dual oxidase 2 and TLR-2 and -4 mRNA. In addition, the model demonstrated the ability to assess the adhesion of specific bacterial strains from the synthetic microbial communitymore specifically, Veillonella parvulato the simulated epithelium. This novel in vitro model may assist in overcoming sampling and retrieval difficulties when studying host-microbiome interactions in the small intestine.},
  author       = {Calatayud Arroyo, Marta and Dezutter, Olivier and Hernandez Sanabria, Emma and Hidalgo Martinez, Silvia and Meysman, Filip and Van de Wiele, Tom},
  issn         = {0892-6638},
  journal      = {FASEB JOURNAL},
  keywords     = {in vitro microbiome,inflammation,Caco-2,HT29-MTX,THP-1,IN-VITRO,EPITHELIAL-CELLS,GENE-EXPRESSION,DENDRITIC CELLS,PARACELLULAR PERMEABILITY,CHEMOKINE CXCL16,BARRIER FUNCTION,COCULTURE MODEL,GUT,CACO-2},
  language     = {eng},
  number       = {3},
  pages        = {3985--3996},
  title        = {Development of a host-microbiome model of the small intestine},
  url          = {http://dx.doi.org/10.1096/fj.201801414r},
  volume       = {33},
  year         = {2019},
}

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