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Species-dependent extracranial manifestations of a brain seeking breast cancer cell line

Valerie De Meulenaere (UGent) , Sara Neyt (UGent) , Bert Vandeghinste (UGent) , Pieter Mollet (UGent) , Olivier De Wever (UGent) , Elke Decrock (UGent) , Luc Leybaert (UGent) , Ingeborg Goethals (UGent) , Christian Vanhove (UGent) , Benedicte Descamps (UGent) , et al.
(2018) PLOS ONE. 13(12).
Author
Organization
Abstract
Purpose: Metastatic brain tumors pose a severe problem in the treatment of patients with breast carcinoma. Preclinical models have been shown to play an important role in unraveling the underlying mechanisms behind the metastatic process and evaluation of new therapeutic approaches. As the size of the rat brain allows improved in vivo imaging, we attempted to establish a rat model for breast cancer brain metastasis that allows follow-up by 7 tesla (7T) preclinical Magnetic Resonance Imaging (MRI). Procedures: Green fluorescent protein-transduced (eGFP) MDA-MB-231br breast cancer cells were labeled with micron-sized particles of iron oxide (MPIOs) and intracardially injected in the left ventricle of female nude rats and mice. 7T preclinical MRI was performed to show the initial distribution of MPIO-labeled cancer cells and to visualize metastasis in the brain. Occurrence of potential metastasis outside the brain was evaluated by 2-deoxy-2-[F-18]fluoro-Dglucose ([F-18]FDG) positron emission tomography (PET)/computed tomography (CT) and potential bone lesions were assessed using [F-18]sodium fluoride ([F-18]NaF) PET/CT. Results: The first signs of brain metastasis development were visible as hyperintensities on T2-weighted (T2w) MR images acquired 3 weeks after intracardiac injection in rats and mice. Early formation of unexpected bone metastasis in rats was clinically observed and assessed using PET/CT. Almost no bone metastasis development was observed in mice after PET/ CT evaluation. Conclusions: Our results suggest that the metastatic propensity of the MDA-MB-231br/eGFP cancer cell line outside the brain is species-dependent. Because of early and abundant formation of bone metastasis with the MDA-MB-231br/eGFP cancer cell line, this rat model is currently not suitable for investigating brain metastasis as a single disease model nor for evaluation of novel brain metastasis treatment strategies.
Keywords
TUMOR BARRIER PERMEABILITY, IN-VIVO, BONE METASTASES, MOUSE MODEL, TRACKING, MRI, PET

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MLA
De Meulenaere, Valerie, Sara Neyt, Bert Vandeghinste, et al. “Species-dependent Extracranial Manifestations of a Brain Seeking Breast Cancer Cell Line.” Ed. Aamir Ahmad. PLOS ONE 13.12 (2018): n. pag. Print.
APA
De Meulenaere, V., Neyt, S., Vandeghinste, B., Mollet, P., De Wever, O., Decrock, E., Leybaert, L., et al. (2018). Species-dependent extracranial manifestations of a brain seeking breast cancer cell line. (A. Ahmad, Ed.)PLOS ONE, 13(12).
Chicago author-date
De Meulenaere, Valerie, Sara Neyt, Bert Vandeghinste, Pieter Mollet, Olivier De Wever, Elke Decrock, Luc Leybaert, et al. 2018. “Species-dependent Extracranial Manifestations of a Brain Seeking Breast Cancer Cell Line.” Ed. Aamir Ahmad. Plos One 13 (12).
Chicago author-date (all authors)
De Meulenaere, Valerie, Sara Neyt, Bert Vandeghinste, Pieter Mollet, Olivier De Wever, Elke Decrock, Luc Leybaert, Ingeborg Goethals, Christian Vanhove, Benedicte Descamps, and Karel Deblaere. 2018. “Species-dependent Extracranial Manifestations of a Brain Seeking Breast Cancer Cell Line.” Ed. Aamir Ahmad. Plos One 13 (12).
Vancouver
1.
De Meulenaere V, Neyt S, Vandeghinste B, Mollet P, De Wever O, Decrock E, et al. Species-dependent extracranial manifestations of a brain seeking breast cancer cell line. Ahmad A, editor. PLOS ONE. 2018;13(12).
IEEE
[1]
V. De Meulenaere et al., “Species-dependent extracranial manifestations of a brain seeking breast cancer cell line,” PLOS ONE, vol. 13, no. 12, 2018.
@article{8585274,
  abstract     = {Purpose: Metastatic brain tumors pose a severe problem in the treatment of patients with breast carcinoma. Preclinical models have been shown to play an important role in unraveling the underlying mechanisms behind the metastatic process and evaluation of new therapeutic approaches. As the size of the rat brain allows improved in vivo imaging, we attempted to establish a rat model for breast cancer brain metastasis that allows follow-up by 7 tesla (7T) preclinical Magnetic Resonance Imaging (MRI). 
Procedures: Green fluorescent protein-transduced (eGFP) MDA-MB-231br breast cancer cells were labeled with micron-sized particles of iron oxide (MPIOs) and intracardially injected in the left ventricle of female nude rats and mice. 7T preclinical MRI was performed to show the initial distribution of MPIO-labeled cancer cells and to visualize metastasis in the brain. Occurrence of potential metastasis outside the brain was evaluated by 2-deoxy-2-[F-18]fluoro-Dglucose ([F-18]FDG) positron emission tomography (PET)/computed tomography (CT) and potential bone lesions were assessed using [F-18]sodium fluoride ([F-18]NaF) PET/CT. 
Results: The first signs of brain metastasis development were visible as hyperintensities on T2-weighted (T2w) MR images acquired 3 weeks after intracardiac injection in rats and mice. Early formation of unexpected bone metastasis in rats was clinically observed and assessed using PET/CT. Almost no bone metastasis development was observed in mice after PET/ CT evaluation. 
Conclusions: Our results suggest that the metastatic propensity of the MDA-MB-231br/eGFP cancer cell line outside the brain is species-dependent. Because of early and abundant formation of bone metastasis with the MDA-MB-231br/eGFP cancer cell line, this rat model is currently not suitable for investigating brain metastasis as a single disease model nor for evaluation of novel brain metastasis treatment strategies.},
  articleno    = {e0208340},
  author       = {De Meulenaere, Valerie and Neyt, Sara and Vandeghinste, Bert and Mollet, Pieter and De Wever, Olivier and Decrock, Elke and Leybaert, Luc and Goethals, Ingeborg and Vanhove, Christian and Descamps, Benedicte and Deblaere, Karel},
  editor       = {Ahmad, Aamir},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  keywords     = {TUMOR BARRIER PERMEABILITY,IN-VIVO,BONE METASTASES,MOUSE MODEL,TRACKING,MRI,PET},
  language     = {eng},
  number       = {12},
  pages        = {16},
  title        = {Species-dependent extracranial manifestations of a brain seeking breast cancer cell line},
  url          = {http://dx.doi.org/10.1371/journal.pone.0208340},
  volume       = {13},
  year         = {2018},
}

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