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Mobile PEAR transcription factors integrate positional cues to prime cambial growth

Shunsuke Miyashima, Pawel Roszak, Iris Sevilem, Koichi Toyokura, Bernhard Blob, Jung-ok Heo, Nathan Mellor, Hanna Help-Rinta-Rahko, Sofia Otero, Wouter Smet (UGent) , et al.
(2019) NATURE. 565(7740). p.490-494
Author
Organization
Abstract
While apical growth in plants initiates upon seed germination, radial growth is only primed during early ontogenesis in procambium cells and activated later by the vascular cambium. Although it is not known how radial growth is organized and regulated in plants, this system resembles the developmental competence observed in some animal systems, in which pre-existing patterns of developmental potential are established early on. Here we show that the initiation of radial growth occurs around early protophloem sieve element (PSE) cell files of the root procambial tissue in Arabidopsis. In this domain, cytokinin signalling promotes expression of novel mobile transcription factors, collectively called PEAR proteins. The PEAR proteins form a short-range concentration gradient peaking at PSE and activating gene expression that promotes radial growth. The expression and function of PEAR proteins are antagonized by well-established polarity transcription factors, HD-ZIP III5, whose expression is concentrated in the more internal domain of radially non-dividing procambial cells by the function of auxin and mobile miR165/166. The PEAR proteins locally promote transcription of their inhibitory HD-ZIP III genes, thereby establishing a negative feedback loop that forms a robust boundary demarking the zone of cell divisions. Taken together, we have established a network, in which the PEAR - HD-ZIP III module integrates spatial information of the hormonal domains and miRNA gradients during root procambial development, to provide adjacent zones of dividing and more quiescent cells as a template for further radial growth.
Keywords
ROOT, GENE, CELLS, EXPRESSION, BIOSYNTHESIS, NETWORKS, REVEALS, SYSTEM, FAMILY, AUXIN

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MLA
Miyashima, Shunsuke, et al. “Mobile PEAR Transcription Factors Integrate Positional Cues to Prime Cambial Growth.” NATURE, vol. 565, no. 7740, 2019, pp. 490–94, doi:10.1038/s41586-018-0839-y.
APA
Miyashima, S., Roszak, P., Sevilem, I., Toyokura, K., Blob, B., Heo, J., … Helariutta, Y. (2019). Mobile PEAR transcription factors integrate positional cues to prime cambial growth. NATURE, 565(7740), 490–494. https://doi.org/10.1038/s41586-018-0839-y
Chicago author-date
Miyashima, Shunsuke, Pawel Roszak, Iris Sevilem, Koichi Toyokura, Bernhard Blob, Jung-ok Heo, Nathan Mellor, et al. 2019. “Mobile PEAR Transcription Factors Integrate Positional Cues to Prime Cambial Growth.” NATURE 565 (7740): 490–94. https://doi.org/10.1038/s41586-018-0839-y.
Chicago author-date (all authors)
Miyashima, Shunsuke, Pawel Roszak, Iris Sevilem, Koichi Toyokura, Bernhard Blob, Jung-ok Heo, Nathan Mellor, Hanna Help-Rinta-Rahko, Sofia Otero, Wouter Smet, Mark Boekschoten, Guido Hooiveld, Kayo Hashimoto, Ondrej Smetana, Riccardo Siligato, Eva-Sophie Wallner, Ari Pekka Mähönen, Yuki Kondo, Charles W Melnyk, Thomas Greb, Keiji Nakajima, Rosangela Sozzani, Anthony Bishopp, Bert De Rybel, and Yka Helariutta. 2019. “Mobile PEAR Transcription Factors Integrate Positional Cues to Prime Cambial Growth.” NATURE 565 (7740): 490–494. doi:10.1038/s41586-018-0839-y.
Vancouver
1.
Miyashima S, Roszak P, Sevilem I, Toyokura K, Blob B, Heo J, et al. Mobile PEAR transcription factors integrate positional cues to prime cambial growth. NATURE. 2019;565(7740):490–4.
IEEE
[1]
S. Miyashima et al., “Mobile PEAR transcription factors integrate positional cues to prime cambial growth,” NATURE, vol. 565, no. 7740, pp. 490–494, 2019.
@article{8584716,
  abstract     = {{While apical growth in plants initiates upon seed germination, radial growth is only primed during early ontogenesis in procambium cells and activated later by the vascular cambium. Although it is not known how radial growth is organized and regulated in plants, this system resembles the developmental competence observed in some animal systems, in which pre-existing patterns of developmental potential are established early on. Here we show that the initiation of radial growth occurs around early protophloem sieve element (PSE) cell files of the root procambial tissue in Arabidopsis. In this domain, cytokinin signalling promotes expression of novel mobile transcription factors, collectively called PEAR proteins. The PEAR proteins form a short-range concentration gradient peaking at PSE and activating gene expression that promotes radial growth. The expression and function of PEAR proteins are antagonized by well-established polarity transcription factors, HD-ZIP III5, whose expression is concentrated in the more internal domain of radially non-dividing procambial cells by the function of auxin and mobile miR165/166. The PEAR proteins locally promote transcription of their inhibitory HD-ZIP III genes, thereby establishing a negative feedback loop that forms a robust boundary demarking the zone of cell divisions. Taken together, we have established a network, in which the PEAR - HD-ZIP III module integrates spatial information of the hormonal domains and miRNA gradients during root procambial development, to provide adjacent zones of dividing and more quiescent cells as a template for further radial growth.}},
  author       = {{Miyashima, Shunsuke and Roszak, Pawel and Sevilem, Iris and Toyokura, Koichi and Blob, Bernhard and Heo, Jung-ok and Mellor, Nathan and Help-Rinta-Rahko, Hanna and Otero, Sofia and Smet, Wouter and Boekschoten, Mark and Hooiveld, Guido and Hashimoto, Kayo and Smetana, Ondrej and Siligato, Riccardo and Wallner, Eva-Sophie and Mähönen, Ari Pekka and Kondo, Yuki and Melnyk, Charles W and Greb, Thomas and Nakajima, Keiji and Sozzani, Rosangela and Bishopp, Anthony and De Rybel, Bert and Helariutta, Yka}},
  issn         = {{0028-0836}},
  journal      = {{NATURE}},
  keywords     = {{ROOT,GENE,CELLS,EXPRESSION,BIOSYNTHESIS,NETWORKS,REVEALS,SYSTEM,FAMILY,AUXIN}},
  language     = {{eng}},
  number       = {{7740}},
  pages        = {{490--494}},
  title        = {{Mobile PEAR transcription factors integrate positional cues to prime cambial growth}},
  url          = {{http://doi.org/10.1038/s41586-018-0839-y}},
  volume       = {{565}},
  year         = {{2019}},
}
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