Advanced search
1 file | 2.15 MB

Auxin function in the brown alga Dictyota dichotoma

Kenny Bogaert (UGent) , Lander Blommaert (UGent) , Karin Ljung, Tom Beeckman (UGent) and Olivier De Clerck (UGent)
(2019) PLANT PHYSIOLOGY. 179(1). p.280-299
Author
Organization
Abstract
Auxin controls body plan patterning in land plants and has been proposed to play a similar role in the development of brown algae (Phaeophyta) despite their distant evolutionary relationship with land plants. The mechanism of auxin action in brown algae remains controversial because of contradicting conclusions derived from pharmacological studies on Fucus. In this study, we used Dictyota dichotoma as a model system to show that auxin plays a role during the apical-basal patterning of the embryo of brown algae. Indole-3-acetic acid (IAA) was detectable in D. dichotoma germlings and mature tissue. Although two-celled D. dichotoma zygotes normally develop a rhizoid from one pole and a thallus meristem from the other, addition of exogenous auxins to one-celled embryos affected polarization, and both poles of the spheroidal embryo developed into rhizoids instead. The effect was strongest at lower pH and when variable extrinsic informational cues were applied. 2-[4-(diethylamino)-2-hydroxybenzoyl]benzoic acid (BUM), an inhibitor of ABC-B/multi-drug resistance/P-glycoprotein (ABCB) subfamily of transporters in land plants, affected rhizoid formation by increasing rhizoid branching and inducing ectopic rhizoids. An in silico survey of auxin genes suggested a diverse range of biosynthesis genes and transport genes, such as PIN-LIKES (PILS) and ATP-binding cassete subfamily (ABCB) transporters, from land plants have homologues in D. dichotoma and Ectocarpus siliculosus. Together with reports on auxin function in basal lineages of green algae, these results suggest that auxin function predates the divergence between the green and brown lineage and the transition towards land plants.
Keywords
METABOLIC NETWORK, EMBRYO DEVELOPMENT, INDOLEACETIC-ACID, PATTERN-FORMATION, FUCUS-DISTICHUS, P-GLYCOPROTEINS, GENOME REVEALS, TRANSPORT, ESTABLISHMENT, POLARITY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 2.15 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Bogaert, Kenny, Lander Blommaert, Karin Ljung, Tom Beeckman, and Olivier De Clerck. 2019. “Auxin Function in the Brown Alga Dictyota Dichotoma.” Plant Physiology 179 (1): 280–299.
APA
Bogaert, Kenny, Blommaert, L., Ljung, K., Beeckman, T., & De Clerck, O. (2019). Auxin function in the brown alga Dictyota dichotoma. PLANT PHYSIOLOGY, 179(1), 280–299.
Vancouver
1.
Bogaert K, Blommaert L, Ljung K, Beeckman T, De Clerck O. Auxin function in the brown alga Dictyota dichotoma. PLANT PHYSIOLOGY. 2019;179(1):280–99.
MLA
Bogaert, Kenny, Lander Blommaert, Karin Ljung, et al. “Auxin Function in the Brown Alga Dictyota Dichotoma.” PLANT PHYSIOLOGY 179.1 (2019): 280–299. Print.
@article{8584711,
  abstract     = {Auxin controls body plan patterning in land plants and has been proposed to play a similar role in the development of brown algae (Phaeophyta) despite their distant evolutionary relationship with land plants. The mechanism of auxin action in brown algae remains controversial because of contradicting conclusions derived from pharmacological studies on Fucus. In this study, we used Dictyota dichotoma as a model system to show that auxin plays a role during the apical-basal patterning of the embryo of brown algae. Indole-3-acetic acid (IAA) was detectable in D. dichotoma germlings and mature tissue. Although two-celled D. dichotoma zygotes normally develop a rhizoid from one pole and a thallus meristem from the other, addition of exogenous auxins to one-celled embryos affected polarization, and both poles of the spheroidal embryo developed into rhizoids instead. The effect was strongest at lower pH and when variable extrinsic informational cues were applied. 2-[4-(diethylamino)-2-hydroxybenzoyl]benzoic acid (BUM), an inhibitor of ABC-B/multi-drug resistance/P-glycoprotein (ABCB) subfamily of transporters in land plants, affected rhizoid formation by increasing rhizoid branching and inducing ectopic rhizoids. An in silico survey of auxin genes suggested a diverse range of biosynthesis genes and transport genes, such as PIN-LIKES (PILS) and ATP-binding cassete subfamily (ABCB) transporters, from land plants have homologues in D. dichotoma and Ectocarpus siliculosus. Together with reports on auxin function in basal lineages of green algae, these results suggest that auxin function predates the divergence between the green and brown lineage and the transition towards land plants.},
  author       = {Bogaert, Kenny and Blommaert, Lander and Ljung, Karin and Beeckman, Tom and De Clerck, Olivier},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {280--299},
  title        = {Auxin function in the brown alga Dictyota dichotoma},
  url          = {http://dx.doi.org/10.1104/pp.18.01041},
  volume       = {179},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: