The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension
- Author
- Marie-France Martin-Eauclaire, Juan Salvatierra, Pascal Mansuelle, Frank Bosmans (UGent) and Pierre E Bougis
- Organization
- Abstract
- We report the detailed chemical, immunological and pharmacological characterization of the -toxin Bot IX from the Moroccan scorpion Buthus occitanus tunetanus venom. Bot IX, which consists of 70 amino acids, is a highly atypical toxin. It carries a unique N-terminal sequence extension and is highly lethal in mice. Voltage clamp recordings on oocytes expressing rat Nav1.2 or insect BgNav1 reveal that, similar to other -like toxins, Bot IX inhibits fast inactivation of both variants. Moreover, Bot IX belongs to the same structural/immunological group as the -like toxin Bot I. Remarkably, radioiodinated Bot IX competes efficiently with the classical -toxin AaH II from Androctonus australis, and displays one of the highest affinities for Nav channels.
- Keywords
- Nav channel, scorpion toxin, alpha-like toxin, ANDROCTONUS-AUSTRALIS-HECTOR, MAMMALIAN SODIUM-CHANNELS, SEA-ANEMONE TOXIN, MOLECULAR-BASIS, VOLTAGE-SENSOR, RAT-BRAIN, BMK M1, FUNCTIONAL SITE, RECEPTOR-SITES
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8584495
- MLA
- Martin-Eauclaire, Marie-France, et al. “The Scorpion Toxin Bot IX Is a Potent Member of the α-like Family and Has a Unique N-Terminal Sequence Extension.” FEBS LETTERS, vol. 590, no. 18, 2016, pp. 3221–32, doi:10.1002/1873-3468.13185.
- APA
- Martin-Eauclaire, M.-F., Salvatierra, J., Mansuelle, P., Bosmans, F., & Bougis, P. E. (2016). The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension. FEBS LETTERS, 590(18), 3221–3232. https://doi.org/10.1002/1873-3468.13185
- Chicago author-date
- Martin-Eauclaire, Marie-France, Juan Salvatierra, Pascal Mansuelle, Frank Bosmans, and Pierre E Bougis. 2016. “The Scorpion Toxin Bot IX Is a Potent Member of the α-like Family and Has a Unique N-Terminal Sequence Extension.” FEBS LETTERS 590 (18): 3221–32. https://doi.org/10.1002/1873-3468.13185.
- Chicago author-date (all authors)
- Martin-Eauclaire, Marie-France, Juan Salvatierra, Pascal Mansuelle, Frank Bosmans, and Pierre E Bougis. 2016. “The Scorpion Toxin Bot IX Is a Potent Member of the α-like Family and Has a Unique N-Terminal Sequence Extension.” FEBS LETTERS 590 (18): 3221–3232. doi:10.1002/1873-3468.13185.
- Vancouver
- 1.Martin-Eauclaire M-F, Salvatierra J, Mansuelle P, Bosmans F, Bougis PE. The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension. FEBS LETTERS. 2016;590(18):3221–32.
- IEEE
- [1]M.-F. Martin-Eauclaire, J. Salvatierra, P. Mansuelle, F. Bosmans, and P. E. Bougis, “The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension,” FEBS LETTERS, vol. 590, no. 18, pp. 3221–3232, 2016.
@article{8584495,
abstract = {{We report the detailed chemical, immunological and pharmacological characterization of the -toxin Bot IX from the Moroccan scorpion Buthus occitanus tunetanus venom. Bot IX, which consists of 70 amino acids, is a highly atypical toxin. It carries a unique N-terminal sequence extension and is highly lethal in mice. Voltage clamp recordings on oocytes expressing rat Nav1.2 or insect BgNav1 reveal that, similar to other -like toxins, Bot IX inhibits fast inactivation of both variants. Moreover, Bot IX belongs to the same structural/immunological group as the -like toxin Bot I. Remarkably, radioiodinated Bot IX competes efficiently with the classical -toxin AaH II from Androctonus australis, and displays one of the highest affinities for Nav channels.}},
author = {{Martin-Eauclaire, Marie-France and Salvatierra, Juan and Mansuelle, Pascal and Bosmans, Frank and Bougis, Pierre E}},
issn = {{0014-5793}},
journal = {{FEBS LETTERS}},
keywords = {{Nav channel,scorpion toxin,alpha-like toxin,ANDROCTONUS-AUSTRALIS-HECTOR,MAMMALIAN SODIUM-CHANNELS,SEA-ANEMONE TOXIN,MOLECULAR-BASIS,VOLTAGE-SENSOR,RAT-BRAIN,BMK M1,FUNCTIONAL SITE,RECEPTOR-SITES}},
language = {{eng}},
number = {{18}},
pages = {{3221--3232}},
title = {{The scorpion toxin Bot IX is a potent member of the α-like family and has a unique N-terminal sequence extension}},
url = {{http://dx.doi.org/10.1002/1873-3468.13185}},
volume = {{590}},
year = {{2016}},
}
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