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Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon

(2018) ARCHIVES OF MICROBIOLOGY. 200(5). p.743-752
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Abstract
Three strains of nitrogen-fixing bacteria isolated from nodules of Inga sp. (INPA54B(T)) and Swartzia sp. (INPA86A and INPA01-91A) in soils under native forest in the Brazilian Amazon were previously identified as belonging to the Bradyrhizobium genus. In this study, these strains were characterized using a polyphasic approach to establish their taxonomic position. The three strains shared more than 99.5% sequence similarity of the 16S rRNA gene with the type strains of five Bradyrhizobium species (B. japonicum USDA 6(T), B. liaoningense LMG 18230(T), B. ottawaense OO99(T), B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T)). However, multilocus sequence analysis of two (recA and glnII) or three (atpD, gyrB, and recA) housekeeping genes indicated that these three strains represent a new Bradyrhizobium species, which is closely related to B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T). DNA-DNA hybridization values between INPA54B(T) and B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T) were only 41.5 and 30.9%, respectively. Phenotypic characterization also allowed the differentiation of the novel species from B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T). In the phylogenetic analysis of the nodC and nifH genes, the three strains showed similar sequences that were divergent from those of type strains of all Bradyrhizobium species. We concluded that these strains represent a novel species, for which the name Bradyrhizobium forestalis is proposed, with INPA54B(T) (= LMG 10044(T)) as type strain. The G+C content in the DNA of INPA54B(T) is 63.7 mol%.
Keywords
CENTROLOBIUM-PARAENSE, ROOT-NODULES, DEOXYRIBONUCLEIC-ACID, MAXIMUM-LIKELIHOOD, GENETIC DIVERSITY, WESTERN AMAZON, DNA, SOILS, HYBRIDIZATION, SEQUENCES, Bradyrhizobium, Biological nitrogen fixation, Polyphasic taxonomy, Symbiotic genes

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Chicago
Martins da Costa, Elaine, Amanda Azarias Guimaraes, Teotonio Soares de Carvalho, Tainara Louzada Rodrigues, Paula Rose de Almeida Ribeiro, Liesbeth Lebbe, Anne Willems, and Fatima M de Souza Moreira. 2018. “Bradyrhizobium Forestalis Sp. Nov., an Efficient Nitrogen-fixing Bacterium Isolated from Nodules of Forest Legume Species in the Amazon.” Archives of Microbiology 200 (5): 743–752.
APA
Martins da Costa, E., Azarias Guimaraes, A., Soares de Carvalho, T., Louzada Rodrigues, T., de Almeida Ribeiro, P. R., Lebbe, L., Willems, A., et al. (2018). Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon. ARCHIVES OF MICROBIOLOGY, 200(5), 743–752.
Vancouver
1.
Martins da Costa E, Azarias Guimaraes A, Soares de Carvalho T, Louzada Rodrigues T, de Almeida Ribeiro PR, Lebbe L, et al. Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon. ARCHIVES OF MICROBIOLOGY. 2018;200(5):743–52.
MLA
Martins da Costa, Elaine et al. “Bradyrhizobium Forestalis Sp. Nov., an Efficient Nitrogen-fixing Bacterium Isolated from Nodules of Forest Legume Species in the Amazon.” ARCHIVES OF MICROBIOLOGY 200.5 (2018): 743–752. Print.
@article{8584254,
  abstract     = {Three strains of nitrogen-fixing bacteria isolated from nodules of Inga sp. (INPA54B(T)) and Swartzia sp. (INPA86A and INPA01-91A) in soils under native forest in the Brazilian Amazon were previously identified as belonging to the Bradyrhizobium genus. In this study, these strains were characterized using a polyphasic approach to establish their taxonomic position. The three strains shared more than 99.5\% sequence similarity of the 16S rRNA gene with the type strains of five Bradyrhizobium species (B. japonicum USDA 6(T), B. liaoningense LMG 18230(T), B. ottawaense OO99(T), B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T)). However, multilocus sequence analysis of two (recA and glnII) or three (atpD, gyrB, and recA) housekeeping genes indicated that these three strains represent a new Bradyrhizobium species, which is closely related to B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T). DNA-DNA hybridization values between INPA54B(T) and B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T) were only 41.5 and 30.9\%, respectively. Phenotypic characterization also allowed the differentiation of the novel species from B. subterraneum 58 2-1(T) and B. yuanmingense LMG 21827(T). In the phylogenetic analysis of the nodC and nifH genes, the three strains showed similar sequences that were divergent from those of type strains of all Bradyrhizobium species. We concluded that these strains represent a novel species, for which the name Bradyrhizobium forestalis is proposed, with INPA54B(T) (= LMG 10044(T)) as type strain. The G+C content in the DNA of INPA54B(T) is 63.7 mol\%.},
  author       = {Martins da Costa, Elaine and Azarias Guimaraes, Amanda and Soares de Carvalho, Teotonio and Louzada Rodrigues, Tainara and de Almeida Ribeiro, Paula Rose and Lebbe, Liesbeth and Willems, Anne and de Souza Moreira, Fatima M},
  issn         = {0302-8933},
  journal      = {ARCHIVES OF MICROBIOLOGY},
  language     = {eng},
  number       = {5},
  pages        = {743--752},
  title        = {Bradyrhizobium forestalis sp. nov., an efficient nitrogen-fixing bacterium isolated from nodules of forest legume species in the Amazon},
  url          = {http://dx.doi.org/10.1007/s00203-018-1486-2},
  volume       = {200},
  year         = {2018},
}

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