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Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934

Yi-hua Sun (UGent) , Paul De Vos (UGent) and Anne Willems (UGent)
Author
Organization
Project
Benckmarking dissimilatory nitrate reduction: focus on nitrate partitioning and nitrous oxide production in Firmicutes.
Project
Understanding biodiversity effects on the functioning of marine benthic ecosystems
Abstract
Until now, the exact mechanisms for N2O production in dissimilatory nitrate/nitrite reduction to ammonium (DNRA) remain underexplored. Previously, we investigated this mechanism in Bacillus licheniformis and Bacillus paralicheniformis, ubiquitous gram-positive bacteria with many industrial applications, and observed significant strain dependency and media dependency in N2O production which was thought to correlate with high residual NO2- . Here, we further studied the influence of several physico-chemical factors on NO3- (or NO2-) partitioning and N2O production in DNRA to shed light on the possible mechanisms of N2O production. The effects of NO3- concentrations under variable or fixed C/N-NO3- ratios, NO2- concentrations under variable or fixed C/N-NO(2)(- )ratios, and NH4+ concentrations under fixed C/N-NO3 ratios were tested during anaerobic incubation of soil bacterium B. paralicheniformis LMG 6934 (previously known as B. licheniformis), a strain with a high nitrite reduction capacity. Monitoring of growth, NO3- , NO2- , NH4+ concentration, and N2O production in physiological tests revealed that NO3- as well as NO(2)(- )concentration showed a linear correlation with N2O production. Increased NO(3)(- )concentration under fixed C/N-NO3- ratios, NO(2)(- )concentration, and NH4+ concentration had a significant positive effect on NO3- (or NO2-) partitioning ([N-NH4+/[N-N2O]) toward N2O, which may be a consequence of the (transient) accumulation and subsequent detoxification of NO2- . These findings extend the information on several physiological parameters affecting DNRA and provide a basis for further study on N2O production during this process.
Keywords
NITROUS-OXIDE PRODUCTION, ESCHERICHIA-COLI, REDUCING BACTERIA, SOIL, NO, FLAVORUBREDOXIN, FLAVOHEMOGLOBIN, TYPHIMURIUM, METABOLISM, CULTURE, ammonification, dissimilatory nitrate/nitrite reduction to ammonium, nitrate respiration, nitrogen assimilation

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Citation

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MLA
Sun, Yi-hua, Paul De Vos, and Anne Willems. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” MICROBIOLOGYOPEN 7.4 (2018): n. pag. Print.
APA
Sun, Yi-hua, De Vos, P., & Willems, A. (2018). Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934. MICROBIOLOGYOPEN, 7(4).
Chicago author-date
Sun, Yi-hua, Paul De Vos, and Anne Willems. 2018. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” Microbiologyopen 7 (4).
Chicago author-date (all authors)
Sun, Yi-hua, Paul De Vos, and Anne Willems. 2018. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” Microbiologyopen 7 (4).
Vancouver
1.
Sun Y, De Vos P, Willems A. Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934. MICROBIOLOGYOPEN. 2018;7(4).
IEEE
[1]
Y. Sun, P. De Vos, and A. Willems, “Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934,” MICROBIOLOGYOPEN, vol. 7, no. 4, 2018.
@article{8584252,
  abstract     = {Until now, the exact mechanisms for N2O production in dissimilatory nitrate/nitrite reduction to ammonium (DNRA) remain underexplored. Previously, we investigated this mechanism in Bacillus licheniformis and Bacillus paralicheniformis, ubiquitous gram-positive bacteria with many industrial applications, and observed significant strain dependency and media dependency in N2O production which was thought to correlate with high residual NO2- . Here, we further studied the influence of several physico-chemical factors on NO3- (or NO2-) partitioning and N2O production in DNRA to shed light on the possible mechanisms of N2O production. The effects of NO3- concentrations under variable or fixed C/N-NO3- ratios, NO2- concentrations under variable or fixed C/N-NO(2)(- )ratios, and NH4+ concentrations under fixed C/N-NO3 ratios were tested during anaerobic incubation of soil bacterium B. paralicheniformis LMG 6934 (previously known as B. licheniformis), a strain with a high nitrite reduction capacity. Monitoring of growth, NO3- , NO2- , NH4+ concentration, and N2O production in physiological tests revealed that NO3- as well as NO(2)(- )concentration showed a linear correlation with N2O production. Increased NO(3)(- )concentration under fixed C/N-NO3- ratios, NO(2)(- )concentration, and NH4+ concentration had a significant positive effect on NO3- (or NO2-) partitioning ([N-NH4+/[N-N2O]) toward N2O, which may be a consequence of the (transient) accumulation and subsequent detoxification of NO2- . These findings extend the information on several physiological parameters affecting DNRA and provide a basis for further study on N2O production during this process.},
  articleno    = {e592},
  author       = {Sun, Yi-hua and De Vos, Paul and Willems, Anne},
  issn         = {2045-8827},
  journal      = {MICROBIOLOGYOPEN},
  keywords     = {NITROUS-OXIDE PRODUCTION,ESCHERICHIA-COLI,REDUCING BACTERIA,SOIL,NO,FLAVORUBREDOXIN,FLAVOHEMOGLOBIN,TYPHIMURIUM,METABOLISM,CULTURE,ammonification,dissimilatory nitrate/nitrite reduction to ammonium,nitrate respiration,nitrogen assimilation},
  language     = {eng},
  number       = {4},
  pages        = {9},
  title        = {Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934},
  url          = {http://dx.doi.org/10.1002/mbo3.592},
  volume       = {7},
  year         = {2018},
}

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