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Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934

Yi-hua Sun (UGent) , Paul De Vos (UGent) and Anne Willems (UGent)
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Abstract
Until now, the exact mechanisms for N2O production in dissimilatory nitrate/nitrite reduction to ammonium (DNRA) remain underexplored. Previously, we investigated this mechanism in Bacillus licheniformis and Bacillus paralicheniformis, ubiquitous gram-positive bacteria with many industrial applications, and observed significant strain dependency and media dependency in N2O production which was thought to correlate with high residual NO2- . Here, we further studied the influence of several physico-chemical factors on NO3- (or NO2-) partitioning and N2O production in DNRA to shed light on the possible mechanisms of N2O production. The effects of NO3- concentrations under variable or fixed C/N-NO3- ratios, NO2- concentrations under variable or fixed C/N-NO(2)(- )ratios, and NH4+ concentrations under fixed C/N-NO3 ratios were tested during anaerobic incubation of soil bacterium B. paralicheniformis LMG 6934 (previously known as B. licheniformis), a strain with a high nitrite reduction capacity. Monitoring of growth, NO3- , NO2- , NH4+ concentration, and N2O production in physiological tests revealed that NO3- as well as NO(2)(- )concentration showed a linear correlation with N2O production. Increased NO(3)(- )concentration under fixed C/N-NO3- ratios, NO(2)(- )concentration, and NH4+ concentration had a significant positive effect on NO3- (or NO2-) partitioning ([N-NH4+/[N-N2O]) toward N2O, which may be a consequence of the (transient) accumulation and subsequent detoxification of NO2- . These findings extend the information on several physiological parameters affecting DNRA and provide a basis for further study on N2O production during this process.
Keywords
NITROUS-OXIDE PRODUCTION, ESCHERICHIA-COLI, REDUCING BACTERIA, SOIL, NO, FLAVORUBREDOXIN, FLAVOHEMOGLOBIN, TYPHIMURIUM, METABOLISM, CULTURE, ammonification, dissimilatory nitrate/nitrite reduction to ammonium, nitrate respiration, nitrogen assimilation

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MLA
Sun, Yi-hua, Paul De Vos, and Anne Willems. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” MICROBIOLOGYOPEN 7.4 (2018): n. pag. Print.
APA
Sun, Yi-hua, De Vos, P., & Willems, A. (2018). Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934. MICROBIOLOGYOPEN, 7(4).
Chicago author-date
Sun, Yi-hua, Paul De Vos, and Anne Willems. 2018. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” Microbiologyopen 7 (4).
Chicago author-date (all authors)
Sun, Yi-hua, Paul De Vos, and Anne Willems. 2018. “Influence of Nitrate and Nitrite Concentration on N2O Production via Dissimilatory Nitrate/nitrite Reduction to Ammonium in Bacillus Paralicheniformis LMG 6934.” Microbiologyopen 7 (4).
Vancouver
1.
Sun Y, De Vos P, Willems A. Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934. MICROBIOLOGYOPEN. 2018;7(4).
IEEE
[1]
Y. Sun, P. De Vos, and A. Willems, “Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934,” MICROBIOLOGYOPEN, vol. 7, no. 4, 2018.
@article{8584252,
  abstract     = {{Until now, the exact mechanisms for N2O production in dissimilatory nitrate/nitrite reduction to ammonium (DNRA) remain underexplored. Previously, we investigated this mechanism in Bacillus licheniformis and Bacillus paralicheniformis, ubiquitous gram-positive bacteria with many industrial applications, and observed significant strain dependency and media dependency in N2O production which was thought to correlate with high residual NO2- . Here, we further studied the influence of several physico-chemical factors on NO3- (or NO2-) partitioning and N2O production in DNRA to shed light on the possible mechanisms of N2O production. The effects of NO3- concentrations under variable or fixed C/N-NO3- ratios, NO2- concentrations under variable or fixed C/N-NO(2)(- )ratios, and NH4+ concentrations under fixed C/N-NO3 ratios were tested during anaerobic incubation of soil bacterium B. paralicheniformis LMG 6934 (previously known as B. licheniformis), a strain with a high nitrite reduction capacity. Monitoring of growth, NO3- , NO2- , NH4+ concentration, and N2O production in physiological tests revealed that NO3- as well as NO(2)(- )concentration showed a linear correlation with N2O production. Increased NO(3)(- )concentration under fixed C/N-NO3- ratios, NO(2)(- )concentration, and NH4+ concentration had a significant positive effect on NO3- (or NO2-) partitioning ([N-NH4+/[N-N2O]) toward N2O, which may be a consequence of the (transient) accumulation and subsequent detoxification of NO2- . These findings extend the information on several physiological parameters affecting DNRA and provide a basis for further study on N2O production during this process.}},
  articleno    = {{e592}},
  author       = {{Sun, Yi-hua and De Vos, Paul and Willems, Anne}},
  issn         = {{2045-8827}},
  journal      = {{MICROBIOLOGYOPEN}},
  keywords     = {{NITROUS-OXIDE PRODUCTION,ESCHERICHIA-COLI,REDUCING BACTERIA,SOIL,NO,FLAVORUBREDOXIN,FLAVOHEMOGLOBIN,TYPHIMURIUM,METABOLISM,CULTURE,ammonification,dissimilatory nitrate/nitrite reduction to ammonium,nitrate respiration,nitrogen assimilation}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{9}},
  title        = {{Influence of nitrate and nitrite concentration on N2O production via dissimilatory nitrate/nitrite reduction to ammonium in Bacillus paralicheniformis LMG 6934}},
  url          = {{http://dx.doi.org/10.1002/mbo3.592}},
  volume       = {{7}},
  year         = {{2018}},
}

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