Passively transferred M2e-specific monoclonal antibody reduces influenza A virus transmission in mice
- Author
- Annasaheb Kolpe, Bert Schepens (UGent) , Liang Ye, Peter Staeheli and Xavier Saelens (UGent)
- Organization
- Abstract
- Influenza represents a global public health threat. Currently available influenza vaccines are effective against strain-matched influenza A and B viruses but do not protect against novel pandemic viruses. Vaccine candidates that target conserved B or T cell epitopes of influenza viruses could circumvent this shortcoming. The conserved extracellular domain of matrix protein 2 (M2e) of influenza A is an example of such a broadly protective vaccine candidate. Protection by M2e-based vaccine candidates largely depends on M2e-specific IgG antibodies. Here we show that the M2e-specific IgG2a monoclonal antibody 65 (MAb 65) can reduce influenza A/Udorn/72 (H3N2) and A/Hong Kong/68 (H3N2) virus plaque formation. This effect was not observed with other influenza A virus strains tested. We further show that passive transfer of MAb 65 to mice can reduce viral loads in the upper and lower airways, which results in reduced transmission of A/Udorn/72 and A/Hong Kong/68 viruses to cohoused, unimmunized contact mice. Virus restriction by passively transferred Mab 65 was significantly less pronounced in Fcgr1(-/-) Fcgr3(-/-) mutant mice compared with wild type controls, suggesting that in vivo protection provided by MAb 65 depends on Fc gamma receptor-mediated antibody effector mechanisms. We conclude that M2e-based antibody immune therapy has the potential to diminish influenza A virus replication in the immunized host as well as in exposed naive contacts.
- Keywords
- Influenza A virus, Mouse transmission model, M2 ectodomain, Broadly protecting antibody, EXTRACELLULAR DOMAIN, MATRIX PROTEIN-2, M2 PROTEIN, HOUSEHOLD TRANSMISSION, AIRBORNE TRANSMISSION, IMMUNE-RESPONSES, MOUSE MODEL, H1N1 VIRUS, INFECTION, PROTECTION
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8583879
- MLA
- Kolpe, Annasaheb, et al. “Passively Transferred M2e-Specific Monoclonal Antibody Reduces Influenza A Virus Transmission in Mice.” ANTIVIRAL RESEARCH, vol. 158, 2018, pp. 244–54, doi:10.1016/j.antiviral.2018.08.017.
- APA
- Kolpe, A., Schepens, B., Ye, L., Staeheli, P., & Saelens, X. (2018). Passively transferred M2e-specific monoclonal antibody reduces influenza A virus transmission in mice. ANTIVIRAL RESEARCH, 158, 244–254. https://doi.org/10.1016/j.antiviral.2018.08.017
- Chicago author-date
- Kolpe, Annasaheb, Bert Schepens, Liang Ye, Peter Staeheli, and Xavier Saelens. 2018. “Passively Transferred M2e-Specific Monoclonal Antibody Reduces Influenza A Virus Transmission in Mice.” ANTIVIRAL RESEARCH 158: 244–54. https://doi.org/10.1016/j.antiviral.2018.08.017.
- Chicago author-date (all authors)
- Kolpe, Annasaheb, Bert Schepens, Liang Ye, Peter Staeheli, and Xavier Saelens. 2018. “Passively Transferred M2e-Specific Monoclonal Antibody Reduces Influenza A Virus Transmission in Mice.” ANTIVIRAL RESEARCH 158: 244–254. doi:10.1016/j.antiviral.2018.08.017.
- Vancouver
- 1.Kolpe A, Schepens B, Ye L, Staeheli P, Saelens X. Passively transferred M2e-specific monoclonal antibody reduces influenza A virus transmission in mice. ANTIVIRAL RESEARCH. 2018;158:244–54.
- IEEE
- [1]A. Kolpe, B. Schepens, L. Ye, P. Staeheli, and X. Saelens, “Passively transferred M2e-specific monoclonal antibody reduces influenza A virus transmission in mice,” ANTIVIRAL RESEARCH, vol. 158, pp. 244–254, 2018.
@article{8583879,
abstract = {{Influenza represents a global public health threat. Currently available influenza vaccines are effective against strain-matched influenza A and B viruses but do not protect against novel pandemic viruses. Vaccine candidates that target conserved B or T cell epitopes of influenza viruses could circumvent this shortcoming. The conserved extracellular domain of matrix protein 2 (M2e) of influenza A is an example of such a broadly protective vaccine candidate. Protection by M2e-based vaccine candidates largely depends on M2e-specific IgG antibodies. Here we show that the M2e-specific IgG2a monoclonal antibody 65 (MAb 65) can reduce influenza A/Udorn/72 (H3N2) and A/Hong Kong/68 (H3N2) virus plaque formation. This effect was not observed with other influenza A virus strains tested. We further show that passive transfer of MAb 65 to mice can reduce viral loads in the upper and lower airways, which results in reduced transmission of A/Udorn/72 and A/Hong Kong/68 viruses to cohoused, unimmunized contact mice. Virus restriction by passively transferred Mab 65 was significantly less pronounced in Fcgr1(-/-) Fcgr3(-/-) mutant mice compared with wild type controls, suggesting that in vivo protection provided by MAb 65 depends on Fc gamma receptor-mediated antibody effector mechanisms. We conclude that M2e-based antibody immune therapy has the potential to diminish influenza A virus replication in the immunized host as well as in exposed naive contacts.}},
author = {{Kolpe, Annasaheb and Schepens, Bert and Ye, Liang and Staeheli, Peter and Saelens, Xavier}},
issn = {{0166-3542}},
journal = {{ANTIVIRAL RESEARCH}},
keywords = {{Influenza A virus,Mouse transmission model,M2 ectodomain,Broadly protecting antibody,EXTRACELLULAR DOMAIN,MATRIX PROTEIN-2,M2 PROTEIN,HOUSEHOLD TRANSMISSION,AIRBORNE TRANSMISSION,IMMUNE-RESPONSES,MOUSE MODEL,H1N1 VIRUS,INFECTION,PROTECTION}},
language = {{eng}},
pages = {{244--254}},
title = {{Passively transferred M2e-specific monoclonal antibody reduces influenza A virus transmission in mice}},
url = {{http://doi.org/10.1016/j.antiviral.2018.08.017}},
volume = {{158}},
year = {{2018}},
}
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