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Porous nanoparticles with self-adjuvanting M2e-fusion protein and recombinant hemagglutinin provide strong and broadly protective immunity against influenza virus infections

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Abstract
Due to the high risk of an outbreak of pandemic influenza, the development of a broadly protective universal influenza vaccine is highly warranted. The design of such a vaccine has attracted attention and much focus has been given to nanoparticle-based influenza vaccines which can be administered intranasally. This is particularly interesting since, contrary to injectable vaccines, mucosal vaccines elicit local IgA and lung resident T cell immunity, which have been found to correlate with stronger protection in experimental models of influenza virus infections. Also, studies in human volunteers have indicated that pre-existing CD4(+) T cells correlate well to increased resistance against infection. We have previously developed a fusion protein with 3 copies of the ectodomain of matrix protein 2 (M2e), which is one of the most explored conserved influenza A virus antigens for a broadly protective vaccine known today. To improve the protective ability of the self-adjuvanting fusion protein, CTA1-3M2e-DD, we incorporated it into porous maltodextrin nanoparticles (NPLs). This proof-of-principle study demonstrates that the combined vaccine vector given intranasally enhanced immune protection against a live challenge infection and reduced the risk of virus transmission between immunized and unimmunized individuals. Most importantly, immune responses to NPLs that also contained recombinant hemagglutinin (HA) were strongly enhanced in a CTA1-enzyme dependentmanner and we achieved broadly protective immunity against a lethal infection with heterosubtypic influenza virus. Immune protection wasmediated by enhanced levels of lung resident CD4(+) T cells as well as anti-HA and -M2e serum IgG and local IgA antibodies.
Keywords
mucosal vaccination, influenza A virus, CTA1-DD, maltodextrin nanoparticles, targeted adjuvant, nasal immunization, Universal vaccine, FOLLICULAR DENDRITIC CELLS, CHOLERA-TOXIN, MUCOSAL VACCINE, T-CELLS, NONNEUTRALIZING ANTIBODIES, DELIVERY, INTRANASAL, RESPONSES, ANTIGEN, IMMUNIZATION

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Citation

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Chicago
Bernasconi, Valentina, Beatrice Bernocchi, Liang Ye, Minh Quan Lê, Ajibola Omokanye, Rodolphe Carpentier, Karin Schön, et al. 2018. “Porous Nanoparticles with Self-adjuvanting M2e-fusion Protein and Recombinant Hemagglutinin Provide Strong and Broadly Protective Immunity Against Influenza Virus Infections.” Frontiers in Immunology 9.
APA
Bernasconi, V., Bernocchi, B., Ye, L., Lê, M. Q., Omokanye, A., Carpentier, R., Schön, K., et al. (2018). Porous nanoparticles with self-adjuvanting M2e-fusion protein and recombinant hemagglutinin provide strong and broadly protective immunity against influenza virus infections. FRONTIERS IN IMMUNOLOGY, 9.
Vancouver
1.
Bernasconi V, Bernocchi B, Ye L, Lê MQ, Omokanye A, Carpentier R, et al. Porous nanoparticles with self-adjuvanting M2e-fusion protein and recombinant hemagglutinin provide strong and broadly protective immunity against influenza virus infections. FRONTIERS IN IMMUNOLOGY. 2018;9.
MLA
Bernasconi, Valentina et al. “Porous Nanoparticles with Self-adjuvanting M2e-fusion Protein and Recombinant Hemagglutinin Provide Strong and Broadly Protective Immunity Against Influenza Virus Infections.” FRONTIERS IN IMMUNOLOGY 9 (2018): n. pag. Print.
@article{8583867,
  abstract     = {Due to the high risk of an outbreak of pandemic influenza, the development of a broadly protective universal influenza vaccine is highly warranted. The design of such a vaccine has attracted attention and much focus has been given to nanoparticle-based influenza vaccines which can be administered intranasally. This is particularly interesting since, contrary to injectable vaccines, mucosal vaccines elicit local IgA and lung resident T cell immunity, which have been found to correlate with stronger protection in experimental models of influenza virus infections. Also, studies in human volunteers have indicated that pre-existing CD4(+) T cells correlate well to increased resistance against infection. We have previously developed a fusion protein with 3 copies of the ectodomain of matrix protein 2 (M2e), which is one of the most explored conserved influenza A virus antigens for a broadly protective vaccine known today. To improve the protective ability of the self-adjuvanting fusion protein, CTA1-3M2e-DD, we incorporated it into porous maltodextrin nanoparticles (NPLs). This proof-of-principle study demonstrates that the combined vaccine vector given intranasally enhanced immune protection against a live challenge infection and reduced the risk of virus transmission between immunized and unimmunized individuals. Most importantly, immune responses to NPLs that also contained recombinant hemagglutinin (HA) were strongly enhanced in a CTA1-enzyme dependentmanner and we achieved broadly protective immunity against a lethal infection with heterosubtypic influenza virus. Immune protection wasmediated by enhanced levels of lung resident CD4(+) T cells as well as anti-HA and -M2e serum IgG and local IgA antibodies.},
  articleno    = {2060},
  author       = {Bernasconi, Valentina and Bernocchi, Beatrice and Ye, Liang and Lê, Minh Quan and Omokanye, Ajibola and Carpentier, Rodolphe and Schön, Karin and Saelens, Xavier and Staeheli, Peter and Betbeder, Didier and Lycke, Nils},
  issn         = {1664-3224},
  journal      = {FRONTIERS IN IMMUNOLOGY},
  keywords     = {mucosal vaccination,influenza A virus,CTA1-DD,maltodextrin nanoparticles,targeted adjuvant,nasal immunization,Universal vaccine,FOLLICULAR DENDRITIC CELLS,CHOLERA-TOXIN,MUCOSAL VACCINE,T-CELLS,NONNEUTRALIZING ANTIBODIES,DELIVERY,INTRANASAL,RESPONSES,ANTIGEN,IMMUNIZATION},
  language     = {eng},
  pages        = {15},
  title        = {Porous nanoparticles with self-adjuvanting M2e-fusion protein and recombinant hemagglutinin provide strong and broadly protective immunity against influenza virus infections},
  url          = {http://dx.doi.org/10.3389/fimmu.2018.02060},
  volume       = {9},
  year         = {2018},
}

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