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A spatiotemporal DNA endoploidy map of the Arabidopsis root reveals roles for the endocycle in root development and stress adaptation

Rahul Bhosale (UGent) , Véronique Boudolf (UGent) , Fabiola Cuevas Bustamante (UGent) , Ran Lu (UGent) , Thomas Eekhout (UGent) , Zhubing Hu (UGent) , Gert Van Isterdael (UGent) , Georgina M Lambert, Fan Xu, Moritz Nowack (UGent) , et al.
(2018) PLANT CELL. 30(10). p.2330-2351
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Abstract
Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but is especially prominent in higher plants, where it has been postulated to be essential for cell growth and fate maintenance. However, a comprehensive understanding of the physiological significance of plant endopolyploidy has remained elusive. Here, we modeled and experimentally verified a high-resolution DNA endoploidy map of the developing Arabidopsis thaliana root, revealing a remarkable spatiotemporal control of DNA endoploidy levels across tissues. Fitting of a simplified model to publicly available data sets profiling root gene expression under various environmental stress conditions suggested that this root endoploidy patterning may be stress-responsive. Furthermore, cellular and transcriptomic analyses revealed that inhibition of endoreplication onset alters the nuclear-to-cellular volume ratio and the expression of cell wall-modifying genes, in correlation with the appearance of cell structural changes. Our data indicate that endopolyploidy might serve to coordinate cell expansion with structural stability and that spatiotemporal endoreplication pattern changes may buffer for stress conditions, which may explain the widespread occurrence of the endocycle in plant species growing in extreme or variable environments.
Keywords
DEPENDENT KINASE INHIBITORS, CELL-SIZE, GENE-EXPRESSION, TRANSCRIPTION, FACTOR, HAIR INITIATION, DUF231 DOMAIN, O-ACETYLATION, FRUIT-GROWTH, ENDOREDUPLICATION, THALIANA

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Chicago
Bhosale, Rahul, Véronique Boudolf, Fabiola Cuevas Bustamante, Ran Lu, Thomas Eekhout, Zhubing Hu, Gert Van Isterdael, et al. 2018. “A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation.” Plant Cell 30 (10): 2330–2351.
APA
Bhosale, R., Boudolf, V., Cuevas Bustamante, F., Lu, R., Eekhout, T., Hu, Z., Van Isterdael, G., et al. (2018). A spatiotemporal DNA endoploidy map of the Arabidopsis root reveals roles for the endocycle in root development and stress adaptation. PLANT CELL, 30(10), 2330–2351.
Vancouver
1.
Bhosale R, Boudolf V, Cuevas Bustamante F, Lu R, Eekhout T, Hu Z, et al. A spatiotemporal DNA endoploidy map of the Arabidopsis root reveals roles for the endocycle in root development and stress adaptation. PLANT CELL. 2018;30(10):2330–51.
MLA
Bhosale, Rahul, Véronique Boudolf, Fabiola Cuevas Bustamante, et al. “A Spatiotemporal DNA Endoploidy Map of the Arabidopsis Root Reveals Roles for the Endocycle in Root Development and Stress Adaptation.” PLANT CELL 30.10 (2018): 2330–2351. Print.
@article{8583860,
  abstract     = {Somatic polyploidy caused by endoreplication is observed in arthropods, molluscs, and vertebrates but is especially prominent in higher plants, where it has been postulated to be essential for cell growth and fate maintenance. However, a comprehensive understanding of the physiological significance of plant endopolyploidy has remained elusive. Here, we modeled and experimentally verified a high-resolution DNA endoploidy map of the developing Arabidopsis thaliana root, revealing a remarkable spatiotemporal control of DNA endoploidy levels across tissues. Fitting of a simplified model to publicly available data sets profiling root gene expression under various environmental stress conditions suggested that this root endoploidy patterning may be stress-responsive. Furthermore, cellular and transcriptomic analyses revealed that inhibition of endoreplication onset alters the nuclear-to-cellular volume ratio and the expression of cell wall-modifying genes, in correlation with the appearance of cell structural changes. Our data indicate that endopolyploidy might serve to coordinate cell expansion with structural stability and that spatiotemporal endoreplication pattern changes may buffer for stress conditions, which may explain the widespread occurrence of the endocycle in plant species growing in extreme or variable environments.},
  author       = {Bhosale, Rahul and Boudolf, V{\'e}ronique and Cuevas Bustamante, Fabiola and Lu, Ran and Eekhout, Thomas and Hu, Zhubing and Van Isterdael, Gert and Lambert, Georgina M and Xu, Fan and Nowack, Moritz and Smith, Richard S and Vercauteren, Ilse and De Rycke, Riet and Storme, Veronique and Beeckman, Tom and Larkin, John C and Kremer, Anna and Hofte, Herman and Galbraith, David W and Kumpf, Robert and Maere, Steven and De Veylder, Lieven},
  issn         = {1040-4651},
  journal      = {PLANT CELL},
  language     = {eng},
  number       = {10},
  pages        = {2330--2351},
  title        = {A spatiotemporal DNA endoploidy map of the Arabidopsis root reveals roles for the endocycle in root development and stress adaptation},
  url          = {http://dx.doi.org/10.1105/tpc.17.00983},
  volume       = {30},
  year         = {2018},
}

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