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Lipid and protein oxidation during in vitro gastrointestinal digestion of pork under Helicobacter pylori gastritis conditions

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Abstract
Helicobacter pylon gastritis affects gastric pH and concentrations of ascorbic acid, hydrogen peroxide, hypochlorite, ammonia and urea, pepsin, and mucin. First, the separate effects of each of these altered factors on oxidation of pork were investigated during in vitro gastrointestinal digestion. Lipid and protein oxidation increased (range 23-48%) in duodenal digests of pork previously exposed to elevated (6.1) versus normal acidic stomach pH (2.3 to 3.5) conditions. Salivary nitrite reduced the formation of lipid and protein oxidation products (range 14-20%) under normal acidic but not elevated stomach pH conditions. Higher amounts of hydrogen peroxide and lower amounts of ascorbic acid decreased concentrations of lipid oxidation products in duodenal pork digests, whereas ammonia slightly stimulated protein oxidation during digestion. Second, two H. pylon gastritis-duodenal digestion models were installed using a set of altered compound concentrations at normal acidic or elevated stomach pH. The elevated pH-gastritis-duodenal digestion model increased pork protein oxidation compared with the normal pH-gastritis and the normal digestion model (14.3 +/- 2.1 vs 8.2 +/- 1.0 nmol DNPH/mg protein, P < 0.001). Compared with the other models, protein oxidation was also increased when nitrite-cured pork was exposed to the elevated pH-gastritis-duodenal digestion model (10.8 +/- 1.4 vs 5.9 +/- 0.8 nmol DNPH/mg protein, P < 0.001), but no significant effect of the model was observed when the pork was seasoned with herbs. Lipid oxidation was not or was marginally affected by the installed model.
Keywords
in vitro digestion model, protein carbonyl compounds, malondialdehyde, 4-hydroxy-2-nonenal, hexanal, OXYGEN SPECIES ACTIVITY, ASCORBIC-ACID, MUCOSAL LIPOPEROXIDATION, AMMONIA PRODUCTION, HYDROGEN-PEROXIDE, NITRIC-OXIDE, VITAMIN-C, INFECTION, STOMACH, JUICE

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Citation

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Chicago
Van Hecke, Thomas, Veronica Basso, and Stefaan De Smet. 2018. “Lipid and Protein Oxidation During in Vitro Gastrointestinal Digestion of Pork Under Helicobacter Pylori Gastritis Conditions.” Journal of Agricultural and Food Chemistry 66 (49): 13000–13010.
APA
Van Hecke, Thomas, Basso, V., & De Smet, S. (2018). Lipid and protein oxidation during in vitro gastrointestinal digestion of pork under Helicobacter pylori gastritis conditions. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 66(49), 13000–13010.
Vancouver
1.
Van Hecke T, Basso V, De Smet S. Lipid and protein oxidation during in vitro gastrointestinal digestion of pork under Helicobacter pylori gastritis conditions. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018;66(49):13000–10.
MLA
Van Hecke, Thomas, Veronica Basso, and Stefaan De Smet. “Lipid and Protein Oxidation During in Vitro Gastrointestinal Digestion of Pork Under Helicobacter Pylori Gastritis Conditions.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.49 (2018): 13000–13010. Print.
@article{8583805,
  abstract     = {Helicobacter pylon gastritis affects gastric pH and concentrations of ascorbic acid, hydrogen peroxide, hypochlorite, ammonia and urea, pepsin, and mucin. First, the separate effects of each of these altered factors on oxidation of pork were investigated during in vitro gastrointestinal digestion. Lipid and protein oxidation increased (range 23-48\%) in duodenal digests of pork previously exposed to elevated (6.1) versus normal acidic stomach pH (2.3 to 3.5) conditions. Salivary nitrite reduced the formation of lipid and protein oxidation products (range 14-20\%) under normal acidic but not elevated stomach pH conditions. Higher amounts of hydrogen peroxide and lower amounts of ascorbic acid decreased concentrations of lipid oxidation products in duodenal pork digests, whereas ammonia slightly stimulated protein oxidation during digestion. Second, two H. pylon gastritis-duodenal digestion models were installed using a set of altered compound concentrations at normal acidic or elevated stomach pH. The elevated pH-gastritis-duodenal digestion model increased pork protein oxidation compared with the normal pH-gastritis and the normal digestion model (14.3 +/- 2.1 vs 8.2 +/- 1.0 nmol DNPH/mg protein, P {\textlangle} 0.001). Compared with the other models, protein oxidation was also increased when nitrite-cured pork was exposed to the elevated pH-gastritis-duodenal digestion model (10.8 +/- 1.4 vs 5.9 +/- 0.8 nmol DNPH/mg protein, P {\textlangle} 0.001), but no significant effect of the model was observed when the pork was seasoned with herbs. Lipid oxidation was not or was marginally affected by the installed model.},
  author       = {Van Hecke, Thomas and Basso, Veronica and De Smet, Stefaan},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  language     = {eng},
  number       = {49},
  pages        = {13000--13010},
  title        = {Lipid and protein oxidation during in vitro gastrointestinal digestion of pork under Helicobacter pylori gastritis conditions},
  url          = {http://dx.doi.org/10.1021/acs.jafc.8b04335},
  volume       = {66},
  year         = {2018},
}

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