@article{8583715,
  abstract     = {{The type III interferon (IFN-lambda) family includes 4 IFN-lambda subtypes in man. In the mouse, only the genes coding for IFN-lambda 2 and -lambda 3 are present. Unlike mouse and human type I IFNs (IFN-alpha/beta), which exhibit strong species specificity, type III IFNs were reported to act in a cross-specific manner. We reexamined the cross-specificity and observed that mouse and human IFN-lambda exhibit some species specificity, although much less than type I IFNs. Mouse IFN-lambda 3 displayed clear species specificity, being 25-fold less active in human cells than the closely related mouse IFN-lambda 2. This specificity likely depends on amino acids in alpha helices A and F that diverged from other IFN-lambda sequences. Human IFN-lambda 4, in contrast, retained high activity in mouse cells. We next developed a firefly luciferase-based reporter cell line, named Fawa-lambda-luc, to detect IFN-lambda in biological fluids with high specificity and sensitivity. Fawa-lambda-luc cells, derived from mouse epithelial cells that are responsive to IFN-lambda, were made nonresponsive to type I IFNs by inactivation of the Ifnar2 gene and strongly responsive to IFN-lambda by overexpression of the mouse IFNLR1. This bioassay was as sensitive as a commercially available enzyme-linked immunosorbent assay in detecting mouse IFN-lambda in cell culture supernatant, as well as in serum and bronchoalveolar lavage samples of virus-infected mice. The assay also enabled the sensitive detection of human IFN-lambda activity, including that of the divergent IFN-lambda 4 with a bias, however, due to variable activity of IFN-lambda subtypes.}},
  author       = {{Jacobs, Sophie and Wavreil, Fanny and Schepens, Bert and Gad, Hans Henrik and Hartmann, Rune and Rocha-Pereira, Joana and Neyts, Johan and Saelens, Xavier and Michiels, Thomas}},
  issn         = {{1079-9907}},
  journal      = {{JOURNAL OF INTERFERON AND CYTOKINE RESEARCH}},
  keywords     = {{species-specific activity,cross-species activity,IFN-lambda receptor,IFN-lambda,type I IFN,antiviral,cytokine,respiratory syncytial virus,IFN-LAMBDA,FUNCTIONAL-ANALYSIS,ANTIVIRAL ACTIVITY,RECEPTOR,VIRUS,INFLAMMATION,PROTECTION,IL-29}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{469--479}},
  title        = {{Species specificity of type III interferon activity and development of a sensitive luciferase-based bioassay for quantitation of mouse interferon-λ}},
  url          = {{http://doi.org/10.1089/jir.2018.0066}},
  volume       = {{38}},
  year         = {{2018}},
}

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