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Enhanced permeability and retention-like extravasation of nanoparticles from the vasculature into tuberculosis granulomas in zebrafish and mouse models

(2018) ACS NANO. 12(8). p.8646-8661
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Abstract
The enhanced permeability and retention (EPR) effect is the only described mechanism enabling nanoparticles (NPs) flowing in blood to reach tumors by a passive targeting mechanism. Here, using the transparent zebrafish model infected with Mycobacterium marinum we show that an EPR-like process also occurs allowing different types of NPs to extravasate from the vasculature to reach granulomas that assemble during tuberculosis (TB) infection. PEGylated liposomes and other NP types cross endothelial barriers near infection sites within minutes after injection and accumulate close to granulomas. Although similar to 100 and 190 nm NPs concentrated most in granulomas, even similar to 700 nm liposomes reached these infection sites in significant numbers. We show by confocal microscopy that NPs can concentrate in small aggregates in foci on the luminal side of the endothelium adjacent to the granulomas. These spots are connected to larger foci of NPs on the ablumenal side of these blood vessels. EM analysis suggests that NPs cross the endothelium via the paracellular route. PEGylated NPs also accumulated efficiently in granulomas in a mouse model of TB infection with Mycobacterium tuberculosis, arguing that the zebrafish embryo model can be used to predict NP behavior in mammalian hosts. In earlier studies we and others showed that uptake of NPs by macrophages that are attracted to infection foci is one pathway for NPs to reach TB granulomas. This study reveals that when NPs are designed to avoid macrophage uptake, they can also efficiently target granulomas via an alternative mechanism that resembles EPR.
Keywords
nanoparticles, tuberculosis, zebrafish, mouse, EPR, mycobacteria, AMPHIPHILIC BLOCK COPOLYPEPT(O)IDES, ENDOTHELIAL GROWTH-FACTOR, MYCOBACTERIUM-BOVIS BCG, TUMOR BLOOD-VESSELS, DRUG-DELIVERY, COPOLYMERS SYNTHESIS, INFECTION, CANCER, SIZE, CELLS

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MLA
Fenaroli, Federico et al. “Enhanced Permeability and Retention-like Extravasation of Nanoparticles from the Vasculature into Tuberculosis Granulomas in Zebrafish and Mouse Models.” ACS NANO 12.8 (2018): 8646–8661. Print.
APA
Fenaroli, F., Repnik, U., Xu, Y., Johann, K., Van Herck, S., Dey, P., Skjeldal, F. M., et al. (2018). Enhanced permeability and retention-like extravasation of nanoparticles from the vasculature into tuberculosis granulomas in zebrafish and mouse models. ACS NANO, 12(8), 8646–8661.
Chicago author-date
Fenaroli, Federico, Urska Repnik, Yitian Xu, Kerstin Johann, Simon Van Herck, Pradip Dey, Frode Miltzov Skjeldal, et al. 2018. “Enhanced Permeability and Retention-like Extravasation of Nanoparticles from the Vasculature into Tuberculosis Granulomas in Zebrafish and Mouse Models.” Acs Nano 12 (8): 8646–8661.
Chicago author-date (all authors)
Fenaroli, Federico, Urska Repnik, Yitian Xu, Kerstin Johann, Simon Van Herck, Pradip Dey, Frode Miltzov Skjeldal, Dominik M Frei, Shahla Bagherifam, Agnese Kocere, Rainer Haag, Bruno De Geest, Matthias Barz, David G Russell, and Gareth Griffiths. 2018. “Enhanced Permeability and Retention-like Extravasation of Nanoparticles from the Vasculature into Tuberculosis Granulomas in Zebrafish and Mouse Models.” Acs Nano 12 (8): 8646–8661.
Vancouver
1.
Fenaroli F, Repnik U, Xu Y, Johann K, Van Herck S, Dey P, et al. Enhanced permeability and retention-like extravasation of nanoparticles from the vasculature into tuberculosis granulomas in zebrafish and mouse models. ACS NANO. 2018;12(8):8646–61.
IEEE
[1]
F. Fenaroli et al., “Enhanced permeability and retention-like extravasation of nanoparticles from the vasculature into tuberculosis granulomas in zebrafish and mouse models,” ACS NANO, vol. 12, no. 8, pp. 8646–8661, 2018.
@article{8583638,
  abstract     = {The enhanced permeability and retention (EPR) effect is the only described mechanism enabling nanoparticles (NPs) flowing in blood to reach tumors by a passive targeting mechanism. Here, using the transparent zebrafish model infected with Mycobacterium marinum we show that an EPR-like process also occurs allowing different types of NPs to extravasate from the vasculature to reach granulomas that assemble during tuberculosis (TB) infection. PEGylated liposomes and other NP types cross endothelial barriers near infection sites within minutes after injection and accumulate close to granulomas. Although similar to 100 and 190 nm NPs concentrated most in granulomas, even similar to 700 nm liposomes reached these infection sites in significant numbers. We show by confocal microscopy that NPs can concentrate in small aggregates in foci on the luminal side of the endothelium adjacent to the granulomas. These spots are connected to larger foci of NPs on the ablumenal side of these blood vessels. EM analysis suggests that NPs cross the endothelium via the paracellular route. PEGylated NPs also accumulated efficiently in granulomas in a mouse model of TB infection with Mycobacterium tuberculosis, arguing that the zebrafish embryo model can be used to predict NP behavior in mammalian hosts. In earlier studies we and others showed that uptake of NPs by macrophages that are attracted to infection foci is one pathway for NPs to reach TB granulomas. This study reveals that when NPs are designed to avoid macrophage uptake, they can also efficiently target granulomas via an alternative mechanism that resembles EPR.},
  author       = {Fenaroli, Federico and Repnik, Urska and Xu, Yitian and Johann, Kerstin and Van Herck, Simon and Dey, Pradip and Skjeldal, Frode Miltzov and Frei, Dominik M and Bagherifam, Shahla and Kocere, Agnese and Haag, Rainer and De Geest, Bruno and Barz, Matthias and Russell, David G and Griffiths, Gareth},
  issn         = {1936-0851},
  journal      = {ACS NANO},
  keywords     = {nanoparticles,tuberculosis,zebrafish,mouse,EPR,mycobacteria,AMPHIPHILIC BLOCK COPOLYPEPT(O)IDES,ENDOTHELIAL GROWTH-FACTOR,MYCOBACTERIUM-BOVIS BCG,TUMOR BLOOD-VESSELS,DRUG-DELIVERY,COPOLYMERS SYNTHESIS,INFECTION,CANCER,SIZE,CELLS},
  language     = {eng},
  number       = {8},
  pages        = {8646--8661},
  title        = {Enhanced permeability and retention-like extravasation of nanoparticles from the vasculature into tuberculosis granulomas in zebrafish and mouse models},
  url          = {http://dx.doi.org/10.1021/acsnano.8b04433},
  volume       = {12},
  year         = {2018},
}

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