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Capacitive sensor for detection of benzo(a)pyrene in water

(2018) TALANTA. 190. p.219-225
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Abstract
An affinity sensor based on capacitive transduction was developed to detect benzo(a)pyrene (BaP) in river water. Two types of recognition elements, the synthetic receptor analogues molecularly imprinted polymers (MIPs) and natural monoclonal antibody (mAb) were tested for this type of biosensor. Different polymerization strategies to obtain MIPs were compared. One approach comprised a preliminary batch synthesis of beads that were subsequently coupled covalently to an electrode surface. Another approach consisted of the in-situ synthesis of MIPs directly onto the electrode surface using electropolymerization. The latter approach provided the best results. To choose the optimal recognition element mAb and MIP-modified electrodes different sets were evaluated with regards to their sensitivity, selectivity, linear range and re-usability. The mAb-modified electrodes were considerably more sensitive toward BaP (ng L-1 range vs mu g L-1 range for the MIP-modified one), and showed a broader linear working range than the MIP-modified electrodes. The latter revealed more suitable for group selective determination of PAHs. The developed capacitive sensor was tested for the detection of BaP in naturally-contaminated water samples collected in different places of Ghent, Belgium. The results obtained with the sensor were coherent and reproducible, and were in a good agreement with the confirmation technique, HPLC-FID.
Keywords
Capacitive biosensor, Molecularly imprinted polymer, Benzo(a)pyrene, Monoclonal anti-benzo(a)pyrene antibody, Water analysis, POLYCYCLIC AROMATIC-HYDROCARBONS, MOLECULARLY IMPRINTED POLYMERS, SOLID-PHASE EXTRACTION, DRINKING WATERS, SAMPLES, FLUORESCENCE, PYRENE, PAHS, IMMUNOSENSOR, IMMUNOASSAY

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MLA
Beloglazova, Natalia, et al. “Capacitive Sensor for Detection of Benzo(a)Pyrene in Water.” TALANTA, vol. 190, 2018, pp. 219–25, doi:10.1016/j.talanta.2018.07.084.
APA
Beloglazova, N., Lenain, P., De Rycke, E., Goryacheva, I. Y., Knopp, D., & De Saeger, S. (2018). Capacitive sensor for detection of benzo(a)pyrene in water. TALANTA, 190, 219–225. https://doi.org/10.1016/j.talanta.2018.07.084
Chicago author-date
Beloglazova, Natalia, Pieterjan Lenain, Esther De Rycke, Irina Yu Goryacheva, Dietmar Knopp, and Sarah De Saeger. 2018. “Capacitive Sensor for Detection of Benzo(a)Pyrene in Water.” TALANTA 190: 219–25. https://doi.org/10.1016/j.talanta.2018.07.084.
Chicago author-date (all authors)
Beloglazova, Natalia, Pieterjan Lenain, Esther De Rycke, Irina Yu Goryacheva, Dietmar Knopp, and Sarah De Saeger. 2018. “Capacitive Sensor for Detection of Benzo(a)Pyrene in Water.” TALANTA 190: 219–225. doi:10.1016/j.talanta.2018.07.084.
Vancouver
1.
Beloglazova N, Lenain P, De Rycke E, Goryacheva IY, Knopp D, De Saeger S. Capacitive sensor for detection of benzo(a)pyrene in water. TALANTA. 2018;190:219–25.
IEEE
[1]
N. Beloglazova, P. Lenain, E. De Rycke, I. Y. Goryacheva, D. Knopp, and S. De Saeger, “Capacitive sensor for detection of benzo(a)pyrene in water,” TALANTA, vol. 190, pp. 219–225, 2018.
@article{8583466,
  abstract     = {{An affinity sensor based on capacitive transduction was developed to detect benzo(a)pyrene (BaP) in river water. Two types of recognition elements, the synthetic receptor analogues molecularly imprinted polymers (MIPs) and natural monoclonal antibody (mAb) were tested for this type of biosensor. Different polymerization strategies to obtain MIPs were compared. One approach comprised a preliminary batch synthesis of beads that were subsequently coupled covalently to an electrode surface. Another approach consisted of the in-situ synthesis of MIPs directly onto the electrode surface using electropolymerization. The latter approach provided the best results. To choose the optimal recognition element mAb and MIP-modified electrodes different sets were evaluated with regards to their sensitivity, selectivity, linear range and re-usability. The mAb-modified electrodes were considerably more sensitive toward BaP (ng L-1 range vs mu g L-1 range for the MIP-modified one), and showed a broader linear working range than the MIP-modified electrodes. The latter revealed more suitable for group selective determination of PAHs. The developed capacitive sensor was tested for the detection of BaP in naturally-contaminated water samples collected in different places of Ghent, Belgium. The results obtained with the sensor were coherent and reproducible, and were in a good agreement with the confirmation technique, HPLC-FID.}},
  author       = {{Beloglazova, Natalia and Lenain, Pieterjan and De Rycke, Esther and Goryacheva, Irina Yu and Knopp, Dietmar and De Saeger, Sarah}},
  issn         = {{0039-9140}},
  journal      = {{TALANTA}},
  keywords     = {{Capacitive biosensor,Molecularly imprinted polymer,Benzo(a)pyrene,Monoclonal anti-benzo(a)pyrene antibody,Water analysis,POLYCYCLIC AROMATIC-HYDROCARBONS,MOLECULARLY IMPRINTED POLYMERS,SOLID-PHASE EXTRACTION,DRINKING WATERS,SAMPLES,FLUORESCENCE,PYRENE,PAHS,IMMUNOSENSOR,IMMUNOASSAY}},
  language     = {{eng}},
  pages        = {{219--225}},
  title        = {{Capacitive sensor for detection of benzo(a)pyrene in water}},
  url          = {{http://doi.org/10.1016/j.talanta.2018.07.084}},
  volume       = {{190}},
  year         = {{2018}},
}

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