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The generation and use of allergen-specific TCR transgenic animals

Manon Vanheerswynghels (UGent) , Wendy Toussaint (UGent) , Martijn Schuijs (UGent) , Leen Vanhoutte (UGent) , Nigel Killeen (UGent) , Hamida Hammad (UGent) and Bart Lambrecht (UGent)
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Abstract
The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.

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MLA
Vanheerswynghels, Manon et al. “The Generation and Use of Allergen-specific TCR Transgenic Animals.” Type 2 Immunity : Methods and Protocols. Ed. R Lee Reinhardt. Vol. 1799. New York, NY, USA: Springer Humana Press, 2018. 183–210. Print.
APA
Vanheerswynghels, M., Toussaint, W., Schuijs, M., Vanhoutte, L., Killeen, N., Hammad, H., & Lambrecht, B. (2018). The generation and use of allergen-specific TCR transgenic animals. In R. L. Reinhardt (Ed.), Type 2 immunity : methods and protocols (Vol. 1799, pp. 183–210). New York, NY, USA: Springer Humana Press.
Chicago author-date
Vanheerswynghels, Manon, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad, and Bart Lambrecht. 2018. “The Generation and Use of Allergen-specific TCR Transgenic Animals.” In Type 2 Immunity : Methods and Protocols, ed. R Lee Reinhardt, 1799:183–210. New York, NY, USA: Springer Humana Press.
Chicago author-date (all authors)
Vanheerswynghels, Manon, Wendy Toussaint, Martijn Schuijs, Leen Vanhoutte, Nigel Killeen, Hamida Hammad, and Bart Lambrecht. 2018. “The Generation and Use of Allergen-specific TCR Transgenic Animals.” In Type 2 Immunity : Methods and Protocols, ed. R Lee Reinhardt, 1799:183–210. New York, NY, USA: Springer Humana Press.
Vancouver
1.
Vanheerswynghels M, Toussaint W, Schuijs M, Vanhoutte L, Killeen N, Hammad H, et al. The generation and use of allergen-specific TCR transgenic animals. In: Reinhardt RL, editor. Type 2 immunity : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 183–210.
IEEE
[1]
M. Vanheerswynghels et al., “The generation and use of allergen-specific TCR transgenic animals,” in Type 2 immunity : methods and protocols, vol. 1799, R. L. Reinhardt, Ed. New York, NY, USA: Springer Humana Press, 2018, pp. 183–210.
@incollection{8583209,
  abstract     = {The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4+ TCR transgenic mice on the Rag2-/- background.},
  author       = {Vanheerswynghels, Manon and Toussaint, Wendy and Schuijs, Martijn and Vanhoutte, Leen and Killeen, Nigel and Hammad, Hamida and Lambrecht, Bart},
  booktitle    = {Type 2 immunity : methods and protocols},
  editor       = {Reinhardt, R Lee},
  isbn         = {9781493978953},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {183--210},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {The generation and use of allergen-specific TCR transgenic animals},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7896-0_15},
  volume       = {1799},
  year         = {2018},
}

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