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Ultra-high-performance supercritical fluid chromatography as a separation tool for Fusarium mycotoxins and their modified forms

(2018) JOURNAL OF AOAC INTERNATIONAL. 101(3). p.627-632
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Abstract
A simple, reliable method for the detection of free and modified Fusarium mycotoxins in beer using state-of-the-art ultra-high-performance supercritical fluid chromatography (UHPSFC) with low-resolution tandem MS (MS/MS) is presented in this paper. The UHPSFC-MS/MS method was developed for nivalenol, deoxynivalenol, 15-acetyl-deoxynivalenol, 3-acetyl-deoxynivalenol, deoxynivalenol-3glucoside, HT-2 toxin, T-2 toxin, T-2 toxin-3glucoside, neosolaniol, diacetoxyscirpenol, zearalenone, a-zearalenol, and beta-zearalenol and their internal standards deepoxy-deoxynivalenol and zearalenone. Due to the broad range of the physicochemical properties of the aforementioned, the sample preparation step was minimized to avoid analyte losses. Extraction with acetonitrile water acetic acid (79 + 20 + 1, v/v/v) and hexane in combination with solid-phase extraction (C18) was followed by a filtration step. After filtration, the extract was evaporated, and the remaining residue was redissolved in a mobile phase for injection (methanol water; 90 + 10, v/v). A mobile phase consisting of supercritical CO2 and a small portion of methanol was used. The developed multimycotoxin method permits the simultaneous determination of multiple fusariotoxins in an one-step chromatographic run using UHPSFC-MS/MS. SFC is a promising strategy; however, the retention mechanism is complex, leading to the unpredictable nature of elution and to some mycotoxins not being retained on the column. This restricts the applicability of UHPSFC in multimycotoxin analyses. The present study is the first report on the use of UHPSFC for the analysis of free and modified Fusarium mycotoxins.
Keywords
MASKED MYCOTOXIN, MASS-SPECTROMETRY, IN-VITRO, LC-MS/MS, DEOXYNIVALENOL, WHEAT, ZEARALENONE, METABOLISM, RESOLUTION, STATE

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Chicago
De Boevre, Marthe, Christof Van Poucke, Emmanuel Njumbe Ediage, Dana Vanderputten, Anita Van Landschoot, and Sarah De Saeger. 2018. “Ultra-high-performance Supercritical Fluid Chromatography as a Separation Tool for Fusarium Mycotoxins and Their Modified Forms.” Journal of Aoac International 101 (3): 627–632.
APA
De Boevre, M., Van Poucke, C., Njumbe Ediage, E., Vanderputten, D., Van Landschoot, A., & De Saeger, S. (2018). Ultra-high-performance supercritical fluid chromatography as a separation tool for Fusarium mycotoxins and their modified forms. JOURNAL OF AOAC INTERNATIONAL, 101(3), 627–632.
Vancouver
1.
De Boevre M, Van Poucke C, Njumbe Ediage E, Vanderputten D, Van Landschoot A, De Saeger S. Ultra-high-performance supercritical fluid chromatography as a separation tool for Fusarium mycotoxins and their modified forms. JOURNAL OF AOAC INTERNATIONAL. 2018;101(3):627–32.
MLA
De Boevre, Marthe, Christof Van Poucke, Emmanuel Njumbe Ediage, et al. “Ultra-high-performance Supercritical Fluid Chromatography as a Separation Tool for Fusarium Mycotoxins and Their Modified Forms.” JOURNAL OF AOAC INTERNATIONAL 101.3 (2018): 627–632. Print.
@article{8582931,
  abstract     = {A simple, reliable method for the detection of free and modified Fusarium mycotoxins in beer using state-of-the-art ultra-high-performance supercritical fluid chromatography (UHPSFC) with low-resolution tandem MS (MS/MS) is presented in this paper. The UHPSFC-MS/MS method was developed for nivalenol, deoxynivalenol, 15-acetyl-deoxynivalenol, 3-acetyl-deoxynivalenol, deoxynivalenol-3glucoside, HT-2 toxin, T-2 toxin, T-2 toxin-3glucoside, neosolaniol, diacetoxyscirpenol, zearalenone, a-zearalenol, and beta-zearalenol and their internal standards deepoxy-deoxynivalenol and zearalenone. Due to the broad range of the physicochemical properties of the aforementioned, the sample preparation step was minimized to avoid analyte losses. Extraction with acetonitrile water acetic acid (79 + 20 + 1, v/v/v) and hexane in combination with solid-phase extraction (C18) was followed by a filtration step. After filtration, the extract was evaporated, and the remaining residue was redissolved in a mobile phase for injection (methanol water; 90 + 10, v/v). A mobile phase consisting of supercritical CO2 and a small portion of methanol was used. The developed multimycotoxin method permits the simultaneous determination of multiple fusariotoxins in an one-step chromatographic run using UHPSFC-MS/MS. SFC is a promising strategy; however, the retention mechanism is complex, leading to the unpredictable nature of elution and to some mycotoxins not being retained on the column. This restricts the applicability of UHPSFC in multimycotoxin analyses. The present study is the first report on the use of UHPSFC for the analysis of free and modified Fusarium mycotoxins.},
  author       = {De Boevre, Marthe and Van Poucke, Christof and Njumbe Ediage, Emmanuel and Vanderputten, Dana and Van Landschoot, Anita and De Saeger, Sarah},
  issn         = {1060-3271},
  journal      = {JOURNAL OF AOAC INTERNATIONAL},
  language     = {eng},
  number       = {3},
  pages        = {627--632},
  title        = {Ultra-high-performance supercritical fluid chromatography as a separation tool for Fusarium mycotoxins and their modified forms},
  url          = {http://dx.doi.org/10.5740/jaoacint.17-0336},
  volume       = {101},
  year         = {2018},
}

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