Advanced search
1 file | 3.07 MB

Toxicokinetics of HT-2 toxin in rats and its metabolic profile in livestock and human liver microsomes

Author
Organization
Abstract
The lack of information on HT-2 toxin leads to inaccurate hazard evaluations. In the present study, toxicokinetic studies of HT-2 toxin were investigated following intravenous (iv) and oral administration to rats at dosages of 1.0 mg per kilogram of body weight. After oral administration, HT-2 toxin was not detected in plasma, whereas its hydroxylated metabolite, 3'-OH HT-2 was identified. Following iv administration, HT-2 toxin; its 3'-hydroxylated product; and its glucuronide derivative, 3-G1cA HT-2, were observed in plasma, and the glucuronide conjugate was the predominant metabolite. To explore the missing HT-2 toxin in plasma, metabolic studies of HT-2 toxin in liver microsomes were conducted. Consequently, eight phase I and three phase II metabolites were identified. Hydroxylation, hydrolysis, and glucuronidation were the main metabolic pathways, among which hydroxylation was the predominant one, mediated by 3A4, a cytochrome P450 enzyme. Additionally, significant interspecies metabolic differences were observed.
Keywords
bioavailability, metabolism, phenotype, mycotoxin, risk assessment, T-2 TOXIN, IN-VITRO, TRICHOTHECENE MYCOTOXINS, FUSARIUM MYCOTOXINS, MASS-SPECTROMETRY, BROILER-CHICKENS, ANIMAL-TISSUES, BIOTRANSFORMATION, DEOXYNIVALENOL, CYTOTOXICITY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.07 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Yang, Shupeng, Huiyan Zhang, Marthe De Boevre, Jinzhen Zhang, Yanshen Li, Suxia Zhang, Sarah De Saeger, Jinhui Zhou, Yi Li, and Feifei Sun. 2018. “Toxicokinetics of HT-2 Toxin in Rats and Its Metabolic Profile in Livestock and Human Liver Microsomes.” Journal of Agricultural and Food Chemistry 66 (30): 8160–8168.
APA
Yang, Shupeng, Zhang, H., De Boevre, M., Zhang, J., Li, Y., Zhang, S., De Saeger, S., et al. (2018). Toxicokinetics of HT-2 toxin in rats and its metabolic profile in livestock and human liver microsomes. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 66(30), 8160–8168.
Vancouver
1.
Yang S, Zhang H, De Boevre M, Zhang J, Li Y, Zhang S, et al. Toxicokinetics of HT-2 toxin in rats and its metabolic profile in livestock and human liver microsomes. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY. 2018;66(30):8160–8.
MLA
Yang, Shupeng, Huiyan Zhang, Marthe De Boevre, et al. “Toxicokinetics of HT-2 Toxin in Rats and Its Metabolic Profile in Livestock and Human Liver Microsomes.” JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 66.30 (2018): 8160–8168. Print.
@article{8582864,
  abstract     = {The lack of information on HT-2 toxin leads to inaccurate hazard evaluations. In the present study, toxicokinetic studies of HT-2 toxin were investigated following intravenous (iv) and oral administration to rats at dosages of 1.0 mg per kilogram of body weight. After oral administration, HT-2 toxin was not detected in plasma, whereas its hydroxylated metabolite, 3'-OH HT-2 was identified. Following iv administration, HT-2 toxin; its 3'-hydroxylated product; and its glucuronide derivative, 3-G1cA HT-2, were observed in plasma, and the glucuronide conjugate was the predominant metabolite. To explore the missing HT-2 toxin in plasma, metabolic studies of HT-2 toxin in liver microsomes were conducted. Consequently, eight phase I and three phase II metabolites were identified. Hydroxylation, hydrolysis, and glucuronidation were the main metabolic pathways, among which hydroxylation was the predominant one, mediated by 3A4, a cytochrome P450 enzyme. Additionally, significant interspecies metabolic differences were observed.},
  author       = {Yang, Shupeng and Zhang, Huiyan and De Boevre, Marthe and Zhang, Jinzhen and Li, Yanshen and Zhang, Suxia and De Saeger, Sarah and Zhou, Jinhui and Li, Yi and Sun, Feifei},
  issn         = {0021-8561},
  journal      = {JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY},
  language     = {eng},
  number       = {30},
  pages        = {8160--8168},
  title        = {Toxicokinetics of HT-2 toxin in rats and its metabolic profile in livestock and human liver microsomes},
  url          = {http://dx.doi.org/10.1021/acs.jafc.8b02893},
  volume       = {66},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: