@article{8582724,
  abstract     = {{The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.}},
  articleno    = {{1535750}},
  author       = {{Théry, Clotilde and Witwer, Kenneth W and Aikawa, Elena and Alcaraz, Maria Jose and Anderson, Johnathon D and Andriantsitohaina, Ramaroson and Antoniou, Anna and Arab, Tanina and Archer, Fabienne and Atkin-Smith, Georgia K and Ayre, D Craig and Bach, Jean-Marie and Bachurski, Daniel and Baharvand, Hossein and Balaj, Leonora and Baldacchino, Shawn and Bauer, Natalie N and Baxter, Amy A and Bebawy, Mary and Beckham, Carla and Bedina Zavec, Apolonija and Benmoussa, Abderrahim and Berardi, Anna C and Bergese, Paolo and Bielska, Ewa and Blenkiron, Cherie and Bobis-Wozowicz, Sylwia and Boilard, Eric and Boireau, Wilfrid and Bongiovanni, Antonella and Borràs, Francesc E and Bosch, Steffi and Boulanger, Chantal M and Breakefield, Xandra and Breglio, Andrew M and Brennan, Meadhbh Á and Brigstock, David R and Brisson, Alain and Broekman, Marike LD and Bromberg, Jacqueline F and Bryl-Górecka, Paulina and Buch, Shilpa and Buck, Amy H and Burger, Dylan and Busatto, Sara and Buschmann, Dominik and Bussolati, Benedetta and Buzás, Edit I and Byrd, James Bryan and Camussi, Giovanni and Carter, David RF and Caruso, Sarah and Chamley, Lawrence W and Chang, Yu-Ting and Chaudhuri, Amrita Datta and Chen, Chihchen and Chen, Shuai and Cheng, Lesley and Chin, Andrew R and Clayton, Aled and Clerici, Stefano P and Cocks, Alex and Cocucci, Emanuele and Coffey, Robert J and Cordeiro-da-Silva, Anabela and Couch, Yvonne and Coumans, Frank AW and Coyle, Beth and Crescitelli, Rossella and Criado, Miria Ferreira and D’Souza-Schorey, Crislyn and Das, Saumya and de Candia, Paola and De Santana, Eliezer F and De Wever, Olivier and del Portillo, Hernando A and Demaret, Tanguy and Deville, Sarah and Devitt, Andrew and Dhondt, Bert and Di Vizio, Dolores and Dieterich, Lothar C and Dolo, Vincenza and Dominguez Rubio, Ana Paula and Dominici, Massimo and Dourado, Mauricio R and Driedonks, Tom AP and Duarte, Filipe V and Duncan, Heather M and Eichenberger, Ramon M and Ekström, Karin and EL Andaloussi, Samir and Elie-Caille, Celine and Erdbrügger, Uta and Falcón-Pérez, Juan M and Fatima, Farah and Fish, Jason E and Flores-Bellver, Miguel and Försönits, András and Frelet-Barrand, Annie and Fricke, Fabia and Fuhrmann, Gregor and Gabrielsson, Susanne and Gámez-Valero, Ana and Gardiner, Chris and Gärtner, Kathrin and Gaudin, Raphael and Gho, Yong Song and Giebel, Bernd and Gilbert, Caroline and Gimona, Mario and Giusti, Ilaria and Goberdhan, Deborah CI and Görgens, André and Gorski, Sharon M and Greening, David W and Gross, Julia Christina and Gualerzi, Alice and Gupta, Gopal N and Gustafson, Dakota and Handberg, Aase and Haraszti, Reka A and Harrison, Paul and Hegyesi, Hargita and Hendrix, An and Hill, Andrew F and Hochberg, Fred H and Hoffmann, Karl F and Holder, Beth and Holthofer, Harry and Hosseinkhani, Baharak and Hu, Guoku and Huang, Yiyao and Huber, Veronica and Hunt, Stuart and Ibrahim, Ahmed Gamal-Eldin and Ikezu, Tsuneya and Inal, Jameel M and Isin, Mustafa and Ivanova, Alena and Jackson, Hannah K and Jacobsen, Soren and Jay, Steven M and Jayachandran, Muthuvel and Jenster, Guido and Jiang, Lanzhou and Johnson, Suzanne M and Jones, Jennifer C and Jong, Ambrose and Jovanovic-Talisman, Tijana and Jung, Stephanie and Kalluri, Raghu and Kano, Shin-ichi and Kaur, Sukhbir and Kawamura, Yumi and Keller, Evan T and Khamari, Delaram and Khomyakova, Elena and Khvorova, Anastasia and Kierulf, Peter and Kim, Kwang Pyo and Kislinger, Thomas and Klingeborn, Mikael and Klinke, David J and Kornek, Miroslaw and Kosanović, Maja M and Kovács, Árpád Ferenc and Krämer-Albers, Eva-Maria and Krasemann, Susanne and Krause, Mirja and Kurochkin, Igor V and Kusuma, Gina D and Kuypers, Sören and Laitinen, Saara and Langevin, Scott M and Languino, Lucia R and Lannigan, Joanne and Lässer, Cecilia and Laurent, Louise C and Lavieu, Gregory and Lázaro-Ibáñez, Elisa and Le Lay, Soazig and Lee, Myung-Shin and Lee, Yi Xin Fiona and Lemos, Debora S and Lenassi, Metka and Leszczynska, Aleksandra and Li, Isaac TS and Liao, Ke and Libregts, Sten F and Ligeti, Erzsebet and Lim, Rebecca and Lim, Sai Kiang and Linē, Aija and Linnemannstöns, Karen and Llorente, Alicia and Lombard, Catherine A and Lorenowicz, Magdalena J and Lörincz, Ákos M and Lötvall, Jan and Lovett, Jason and Lowry, Michelle C and Loyer, Xavier and Lu, Quan and Lukomska, Barbara and Lunavat, Taral R and Maas, Sybren LN and Malhi, Harmeet and Marcilla, Antonio and Mariani, Jacopo and Mariscal, Javier and Martens-Uzunova, Elena S and Martin-Jaular, Lorena and Martinez, M Carmen and Martins, Vilma Regina and Mathieu, Mathilde and Mathivanan, Suresh and Maugeri, Marco and McGinnis, Lynda K and McVey, Mark J and Meckes, David G and Meehan, Katie L and Mertens, Inge and Minciacchi, Valentina R and Möller, Andreas and Møller Jørgensen, Malene and Morales-Kastresana, Aizea and Morhayim, Jess and Mullier, François and Muraca, Maurizio and Musante, Luca and Mussack, Veronika and Muth, Dillon C and Myburgh, Kathryn H and Najrana, Tanbir and Nawaz, Muhammad and Nazarenko, Irina and Nejsum, Peter and Neri, Christian and Neri, Tommaso and Nieuwland, Rienk and Nimrichter, Leonardo and Nolan, John P and Nolte-’t Hoen, Esther NM and Hooten, Nicole Noren and O’Driscoll, Lorraine and O’Grady, Tina and O’Loghlen, Ana and Ochiya, Takahiro and Olivier, Martin and Ortiz, Alberto and Ortiz, Luis A and Osteikoetxea, Xabier and Ostegaard, Ole and Ostrowski, Matias and Park, Jaesung and Pegtel, D. Michiel and Peinado, Hector and Perut, Francesca and Pfaffl, Michael W and Phinney, Donald G and Pieters, Bartijn CH and Pink, Ryan C and Pisetsky, David S and Pogge von Strandmann, Elke and Polakovicova, Iva and Poon, Ivan KH and Powell, Bonita H and Prada, Ilaria and Pulliam, Lynn and Quesenberry, Peter and Radeghieri, Annalisa and Raffai, Robert L and Raimondo, Stefania and Rak, Janusz and Ramirez, Marcel I and Raposo, Graça and Rayyan, Morsi S and Regev-Rudzki, Neta and Ricklefs, Franz L and Robbins, Paul D and Roberts, David D and Rodrigues, Silvia C and Rohde, Eva and Rome, Sophie and Rouschop, Kasper MA and Rughetti, Aurelia and Russell, Ashley E and Saá, Paula and Sahoo, Susmita and Salas-Huenuleo, Edison and Sánchez, Catherine and Saugstad, Julie A and Saul, Meike J and Schiffelers, Raymond M and Schneider, Raphael and Schøyen, Tine Hiorth and Scott, Aaron and Shahaj, Eriomina and Sharma, Shivani and Shatnyeva, Olga and Shekari, Faezeh and Shelke, Ganesh Vilas and Shetty, Ashok K and Shiba, Kiyotaka and Siljander, Pia R-M and Silva, Andreia M and Skowronek, Agata and Snyder, Orman L and Soares, Rodrigo Pedro and Sódar, Barbara W and Soekmadji, Carolina and Sotillo, Javier and Stahl, Philip D and Stoorvogel, Willem and Stott, Shannon L and Strasser, Erwin F and Swift, Simon and Tahara, Hidetoshi and Tewari, Muneesh and Timms, Kate and Tiwari, Swasti and Tixeira, Rochelle and Tkach, Mercedes and Toh, Wei Seong and Tomasini, Richard and Torrecilhas, Ana Claudia and Tosar, Juan Pablo and Toxavidis, Vasilis and Urbanelli, Lorena and Vader, Pieter and van Balkom, Bas WM and van der Grein, Susanne G and Van Deun, Jan and van Herwijnen, Martijn JC and Van Keuren-Jensen, Kendall and van Niel, Guillaume and van Royen, Martin E and van Wijnen, Andre J and Vasconcelos, M Helena and Vechetti, Ivan J and Veit, Tiago D and Vella, Laura J and Velot, Émilie and Verweij, Frederik J and Vestad, Beate and Viñas, Jose L and Visnovitz, Tamás and Vukman, Krisztina V and Wahlgren, Jessica and Watson, Dionysios C and Wauben, Marca HM and Weaver, Alissa and Webber, Jason P and Weber, Viktoria and Wehman, Ann M and Weiss, Daniel J and Welsh, Joshua A and Wendt, Sebastian and Wheelock, Asa M and Wiener, Zoltán and Witte, Leonie and Wolfram, Joy and Xagorari, Angeliki and Xander, Patricia and Xu, Jing and Yan, Xiaomei and Yáñez-Mó, María and Yin, Hang and Yuana, Yuana and Zappulli, Valentina and Zarubova, Jana and Žėkas, Vytautas and Zhang, Jian-ye and Zhao, Zezhou and Zheng, Lei and Zheutlin, Alexander R and Zickler, Antje M and Zimmermann, Pascale and Zivkovic, Angela M and Zocco, Davide and Zuba-Surma, Ewa K}},
  issn         = {{2001-3078}},
  journal      = {{JOURNAL OF EXTRACELLULAR VESICLES}},
  keywords     = {{extracellular vesicles,exosomes,ectosomes,microvesicles,minimal information requirements,guidelines,standardization,microparticles,rigor,reproducibility,CELL-DERIVED MICROPARTICLES,SURFACE-PLASMON RESONANCE,SIZE-EXCLUSION CHROMATOGRAPHY,FIELD-FLOW FRACTIONATION,HUMAN URINARY EXOSOMES,PROSTATE-CANCER,MEMBRANE-VESICLES,FETAL BOVINE,CIRCULATING MICROPARTICLES,PREANALYTICAL PARAMETERS}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{43}},
  title        = {{Minimal information for studies of extracellular vesicles 2018 (MISEV2018) : a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines}},
  url          = {{http://doi.org/10.1080/20013078.2018.1535750}},
  volume       = {{7}},
  year         = {{2018}},
}

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