CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans
(2019)
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES.
74(8).
p.1198-1205
- Author
- Huaihan Cai (UGent) , Ineke Dhondt (UGent) , Lieselot Vandemeulebroucke, Caroline Vlaeminck (UGent) , Madina Rasulova (UGent) and Bart Braeckman (UGent)
- Organization
- Abstract
- When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span-doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals' food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.
- Keywords
- biology of aging, Caenorhabditis elegans, dietary restriction, DIETARY RESTRICTION, C-ELEGANS, LONGEVITY, EXTENSION, GENE, DIFFERENTIATION, TRANSCRIPTION, REQUIREMENT, PATHWAYS, DAF-16
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8582566
- MLA
- Cai, Huaihan, et al. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, vol. 74, no. 8, 2019, pp. 1198–205, doi:10.1093/gerona/glx206.
- APA
- Cai, H., Dhondt, I., Vandemeulebroucke, L., Vlaeminck, C., Rasulova, M., & Braeckman, B. (2019). CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 74(8), 1198–1205. https://doi.org/10.1093/gerona/glx206
- Chicago author-date
- Cai, Huaihan, Ineke Dhondt, Lieselot Vandemeulebroucke, Caroline Vlaeminck, Madina Rasulova, and Bart Braeckman. 2019. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 74 (8): 1198–1205. https://doi.org/10.1093/gerona/glx206.
- Chicago author-date (all authors)
- Cai, Huaihan, Ineke Dhondt, Lieselot Vandemeulebroucke, Caroline Vlaeminck, Madina Rasulova, and Bart Braeckman. 2019. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 74 (8): 1198–1205. doi:10.1093/gerona/glx206.
- Vancouver
- 1.Cai H, Dhondt I, Vandemeulebroucke L, Vlaeminck C, Rasulova M, Braeckman B. CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES. 2019;74(8):1198–205.
- IEEE
- [1]H. Cai, I. Dhondt, L. Vandemeulebroucke, C. Vlaeminck, M. Rasulova, and B. Braeckman, “CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans,” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, vol. 74, no. 8, pp. 1198–1205, 2019.
@article{8582566,
abstract = {{When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span-doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals' food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.}},
author = {{Cai, Huaihan and Dhondt, Ineke and Vandemeulebroucke, Lieselot and Vlaeminck, Caroline and Rasulova, Madina and Braeckman, Bart}},
issn = {{1079-5006}},
journal = {{JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES}},
keywords = {{biology of aging,Caenorhabditis elegans,dietary restriction,DIETARY RESTRICTION,C-ELEGANS,LONGEVITY,EXTENSION,GENE,DIFFERENTIATION,TRANSCRIPTION,REQUIREMENT,PATHWAYS,DAF-16}},
language = {{eng}},
number = {{8}},
pages = {{1198--1205}},
title = {{CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans}},
url = {{http://doi.org/10.1093/gerona/glx206}},
volume = {{74}},
year = {{2019}},
}
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