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CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans

Huaihan Cai (UGent) , Ineke Dhondt (UGent) , Lieselot Vandemeulebroucke (UGent) , Caroline Vlaeminck (UGent) , Madina Rasulova (UGent) and Bart Braeckman (UGent)
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Abstract
When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span-doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals' food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.
Keywords
biology of aging, Caenorhabditis elegans, dietary restriction, DIETARY RESTRICTION, C-ELEGANS, LONGEVITY, EXTENSION, GENE, DIFFERENTIATION, TRANSCRIPTION, REQUIREMENT, PATHWAYS, DAF-16

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MLA
Cai, Huaihan, et al. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, vol. 74, no. 8, 2019, pp. 1198–205, doi:10.1093/gerona/glx206.
APA
Cai, H., Dhondt, I., Vandemeulebroucke, L., Vlaeminck, C., Rasulova, M., & Braeckman, B. (2019). CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, 74(8), 1198–1205. https://doi.org/10.1093/gerona/glx206
Chicago author-date
Cai, Huaihan, Ineke Dhondt, Lieselot Vandemeulebroucke, Caroline Vlaeminck, Madina Rasulova, and Bart Braeckman. 2019. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 74 (8): 1198–1205. https://doi.org/10.1093/gerona/glx206.
Chicago author-date (all authors)
Cai, Huaihan, Ineke Dhondt, Lieselot Vandemeulebroucke, Caroline Vlaeminck, Madina Rasulova, and Bart Braeckman. 2019. “CBP-1 Acts in GABAergic Neurons to Double Life Span in Axenically Cultured Caenorhabditis Elegans.” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES 74 (8): 1198–1205. doi:10.1093/gerona/glx206.
Vancouver
1.
Cai H, Dhondt I, Vandemeulebroucke L, Vlaeminck C, Rasulova M, Braeckman B. CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans. JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES. 2019;74(8):1198–205.
IEEE
[1]
H. Cai, I. Dhondt, L. Vandemeulebroucke, C. Vlaeminck, M. Rasulova, and B. Braeckman, “CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans,” JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES, vol. 74, no. 8, pp. 1198–1205, 2019.
@article{8582566,
  abstract     = {When cultured in axenic medium, Caenorhabditis elegans shows the largest life-span extension compared with other dietary restriction regimens. However, the underlying molecular mechanism still remains elusive. The gene cbp-1, encoding the worm ortholog of p300/CBP (CREB-binding protein), is one of the very few key genes known to be essential for life span doubling under axenic dietary restriction (ADR). By using tissue-specific RNAi, we found that cbp-1 expression in the germline is essential for fertility, whereas this gene functions specifically in the GABAergic neurons to support the full life span-doubling effect of ADR. Surprisingly, GABA itself is not required for ADR-induced longevity, suggesting a role of neuropeptide signaling. In addition, chemotaxis assays illustrate that neuronal inactivation of CBP-1 affects the animals' food sensing behavior. Together, our results show that the strong life-span extension in axenic medium is under strict control of GABAergic neurons and may be linked to food sensing.},
  author       = {Cai, Huaihan and Dhondt, Ineke and Vandemeulebroucke, Lieselot and Vlaeminck, Caroline and Rasulova, Madina and Braeckman, Bart},
  issn         = {1079-5006},
  journal      = {JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES},
  keywords     = {biology of aging,Caenorhabditis elegans,dietary restriction,DIETARY RESTRICTION,C-ELEGANS,LONGEVITY,EXTENSION,GENE,DIFFERENTIATION,TRANSCRIPTION,REQUIREMENT,PATHWAYS,DAF-16},
  language     = {eng},
  number       = {8},
  pages        = {1198--1205},
  title        = {CBP-1 acts in GABAergic neurons to double life span in axenically cultured Caenorhabditis elegans},
  url          = {http://dx.doi.org/10.1093/gerona/glx206},
  volume       = {74},
  year         = {2019},
}

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