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FRET monitoring of intracellular ketal hydrolysis in synthetic nanoparticles

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Abstract
Degradable synthetic crosslinking is a versatile strategy to harness nanomaterials against disassembly in a complex physiological medium prompted by dilution effects or competitive interaction. In particular, chemical bonds such as ketals that are stable at physiological conditions but are cleaved in response to disease-mediated or intracellular conditions (e.g., a mildly acidic pH) are of great relevance for biomedical applications. Despite the range of spectroscopic or chromatographic analyses methods that allow chemical degradation in solution to be assessed, it is much less straightforward to interrogate synthetic nanomaterials for their degradation state when located inside a living organism. We demonstrate a method based on FRET analysis to monitor intracellular disassembly of block-copolymer-derived nanoparticles engineered with a FRET couple on separate polymer chains, which after self-assembly are covalently crosslinked with a pH-sensitive ketal-containing crosslinker.
Keywords
CATIONIC NANOHYDROGEL PARTICLES, LINKED POLYMERIC MICELLES, DRUG-DELIVERY, BIOMEDICAL APPLICATIONS, COPOLYMER MICELLES, PH VALUES, NANOGELS, RELEASE, degradable linkers, fluorescence, FRET, nanoparticles, pH-responsive, materials

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Chicago
Nuhn, Lutz, Simon Van Herck, Andreas Best, Kim Deswarte, Maria Kokkinopoulou, Ingo Lieberwirth, Kaloian Koynov, Bart Lambrecht, and Bruno De Geest. 2018. “FRET Monitoring of Intracellular Ketal Hydrolysis in Synthetic Nanoparticles.” Angewandte Chemie-international Edition 57 (33): 10760–10764.
APA
Nuhn, L., Van Herck, S., Best, A., Deswarte, K., Kokkinopoulou, M., Lieberwirth, I., Koynov, K., et al. (2018). FRET monitoring of intracellular ketal hydrolysis in synthetic nanoparticles. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 57(33), 10760–10764.
Vancouver
1.
Nuhn L, Van Herck S, Best A, Deswarte K, Kokkinopoulou M, Lieberwirth I, et al. FRET monitoring of intracellular ketal hydrolysis in synthetic nanoparticles. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION. 2018;57(33):10760–4.
MLA
Nuhn, Lutz, Simon Van Herck, Andreas Best, et al. “FRET Monitoring of Intracellular Ketal Hydrolysis in Synthetic Nanoparticles.” ANGEWANDTE CHEMIE-INTERNATIONAL EDITION 57.33 (2018): 10760–10764. Print.
@article{8582278,
  abstract     = {Degradable synthetic crosslinking is a versatile strategy to harness nanomaterials against disassembly in a complex physiological medium prompted by dilution effects or competitive interaction. In particular, chemical bonds such as ketals that are stable at physiological conditions but are cleaved in response to disease-mediated or intracellular conditions (e.g., a mildly acidic pH) are of great relevance for biomedical applications. Despite the range of spectroscopic or chromatographic analyses methods that allow chemical degradation in solution to be assessed, it is much less straightforward to interrogate synthetic nanomaterials for their degradation state when located inside a living organism. We demonstrate a method based on FRET analysis to monitor intracellular disassembly of block-copolymer-derived nanoparticles engineered with a FRET couple on separate polymer chains, which after self-assembly are covalently crosslinked with a pH-sensitive ketal-containing crosslinker.},
  author       = {Nuhn, Lutz and Van Herck, Simon and Best, Andreas and Deswarte, Kim and Kokkinopoulou, Maria and Lieberwirth, Ingo and Koynov, Kaloian and Lambrecht, Bart and De Geest, Bruno},
  issn         = {1433-7851},
  journal      = {ANGEWANDTE CHEMIE-INTERNATIONAL EDITION},
  language     = {eng},
  number       = {33},
  pages        = {10760--10764},
  title        = {FRET monitoring of intracellular ketal hydrolysis in synthetic nanoparticles},
  url          = {http://dx.doi.org/10.1002/anie.201803847},
  volume       = {57},
  year         = {2018},
}

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