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Dysregulation of NF-kB in glandular epithelial cells results in Sjögren's-like features

(2018) PLOS ONE. 13(8).
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Abstract
The autoimmune disease primary Sjogren's syndrome (pSS) is characterized by hypofunction of the salivary glands (SGs), the cause of which is not correlated to lymphocytic SG infiltration, as prevailing dogma often states. We knocked out the NF-kappa B proinflammatory pathway inhibitor A20 in keratin14(+) epithelial cells, to investigate if immune activated epithelial cells are capable of initiating pSS SG hallmarks. We show that immune activated epithelial cells can cause T cell dominated leukocytic infiltration and immune foci development of the SGs, reflecting the early clinical picture. Infiltrating leukocytes invaded striated ducts, similar to early stage lymphoepithelial lesions observed clinically. Expression of proinflammatory cyto-/chemokines IFN gamma TNF alpha, IL-6, CXCL10 and CXCL13 increased in A20(-/-) SGs, and functionally both volume and mucin 10 content of whole stimulated saliva from A20(-/-) mice was significantly reduced. Epithelial cells may therefore represent the initial trigger for pSS SG pathologies, as opposed to simple reactionaries to pre-existing stimuli.
Keywords
MESSENGER-RNA EXPRESSION, ACTIVATION

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Please use this url to cite or link to this publication:

Chicago
Wang, Xiaoyan, Abeer Shaalan, Silvia Liefers, Julie Coudenys, Dirk Elewaut, Gordon B Proctor, Hendrika Bootsma, Frans GM Kroese, and Sarah Pringle. 2018. “Dysregulation of NF-kB in Glandular Epithelial Cells Results in Sjögren’s-like Features.” Plos One 13 (8).
APA
Wang, Xiaoyan, Shaalan, A., Liefers, S., Coudenys, J., Elewaut, D., Proctor, G. B., Bootsma, H., et al. (2018). Dysregulation of NF-kB in glandular epithelial cells results in Sjögren’s-like features. PLOS ONE, 13(8).
Vancouver
1.
Wang X, Shaalan A, Liefers S, Coudenys J, Elewaut D, Proctor GB, et al. Dysregulation of NF-kB in glandular epithelial cells results in Sjögren’s-like features. PLOS ONE. 2018;13(8).
MLA
Wang, Xiaoyan, Abeer Shaalan, Silvia Liefers, et al. “Dysregulation of NF-kB in Glandular Epithelial Cells Results in Sjögren’s-like Features.” PLOS ONE 13.8 (2018): n. pag. Print.
@article{8582241,
  abstract     = {The autoimmune disease primary Sjogren's syndrome (pSS) is characterized by hypofunction of the salivary glands (SGs), the cause of which is not correlated to lymphocytic SG infiltration, as prevailing dogma often states. We knocked out the NF-kappa B proinflammatory pathway inhibitor A20 in keratin14(+) epithelial cells, to investigate if immune activated epithelial cells are capable of initiating pSS SG hallmarks. We show that immune activated epithelial cells can cause T cell dominated leukocytic infiltration and immune foci development of the SGs, reflecting the early clinical picture. Infiltrating leukocytes invaded striated ducts, similar to early stage lymphoepithelial lesions observed clinically. Expression of proinflammatory cyto-/chemokines IFN gamma TNF alpha, IL-6, CXCL10 and CXCL13 increased in A20(-/-) SGs, and functionally both volume and mucin 10 content of whole stimulated saliva from A20(-/-) mice was significantly reduced. Epithelial cells may therefore represent the initial trigger for pSS SG pathologies, as opposed to simple reactionaries to pre-existing stimuli.},
  articleno    = {e0200212},
  author       = {Wang, Xiaoyan and Shaalan, Abeer and Liefers, Silvia and Coudenys, Julie and Elewaut, Dirk and Proctor, Gordon B and Bootsma, Hendrika and Kroese, Frans GM and Pringle, Sarah},
  issn         = {1932-6203},
  journal      = {PLOS ONE},
  language     = {eng},
  number       = {8},
  pages        = {9},
  title        = {Dysregulation of NF-kB in glandular epithelial cells results in Sj{\"o}gren's-like features},
  url          = {http://dx.doi.org/10.1371/journal.pone.0200212},
  volume       = {13},
  year         = {2018},
}

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