
Staphylococcus aureus induces a mucosal type 2 immune response via epithelial cell-derived cytokines
- Author
- Feng Lan (UGent) , Nan Zhang (UGent) , Gabriële Holtappels (UGent) , Natalie De Ruyck (UGent) , Olga Krysko (UGent) , Koen Van Crombruggen (UGent) , Harald Braun (UGent) , Sebastian L Johnston, Nikos G Papadopoulos, Luo Zhang and Claus Bachert (UGent)
- Organization
- Abstract
- Rationale: Chronic rhinosinusitis with nasal polyps is characterized by a T-helper cell type 2-skewed upper airway inflammation. Mucosal Staphylococcus aureus colonization is found in the majority of patients with nasal polyps. S. aureus is known to induce type 2 cytokine release via enterotoxins. Objectives: To investigate the impact of non-enterotoxin-producing S. aureus on type 2 cytokine release. Methods: TSLP (thymic stromal lymphopoietin), IL-33, and type 2 cytokines were assessed in a human mucosal tissue model upon S. aureus infection. Measurements and Main Results: S. aureus exposure increased the expression of IL-33, TSLP, IL-5, and IL-13 in nasal polyp tissue, accompanied by elevated expression levels of TSLP and IL-33 receptors, predominantly on CD3(+) T cells. S. aureus infection led to the release of TSLP, but not IL-33, IL-5, or IL-13, from healthy inferior turbinate tissue. In contrast, S. epidermidis did not induce any epithelial cell-derived cytokines in nasal polyp or healthy tissue. S. aureus infection also increased the release of IL-33 and TSLP in BEAS-2B epithelial cells, accompanied by activation of NF-kappa B (nuclear factor kB) pathways. Incubation with CU-CPT22, a specific Toll-like receptor 2 antagonist, significantly reduced the S. aureus-induced release of TSLP and IL-33, and the activity of theNF-kappa B signal in BEAS-2B cells. Conclusions: This study demonstrates for the first time that S. aureus can directly induce epithelial cell-derived cytokine release via binding to Toll-like receptor 2, and may thereby propagate type 2 cytokine expression in nasal polyp tissue.
- Keywords
- chronic rhinosinusitis with nasal polyps, IL-33, Staphylococcus aureus, Th2, thymic stromal lymphopoietin, THYMIC STROMAL LYMPHOPOIETIN, NASAL POLYP DISEASE, CHRONIC RHINOSINUSITIS, HUMAN KERATINOCYTES, ENTEROTOXIN-B, INFLAMMATION, ASTHMA, IL-33, PROTEIN, TSLP
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8582199
- MLA
- Lan, Feng, et al. “Staphylococcus Aureus Induces a Mucosal Type 2 Immune Response via Epithelial Cell-Derived Cytokines.” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, vol. 198, no. 4, 2018, pp. 452–63, doi:10.1164/rccm.201710-2112oc.
- APA
- Lan, F., Zhang, N., Holtappels, G., De Ruyck, N., Krysko, O., Van Crombruggen, K., … Bachert, C. (2018). Staphylococcus aureus induces a mucosal type 2 immune response via epithelial cell-derived cytokines. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 198(4), 452–463. https://doi.org/10.1164/rccm.201710-2112oc
- Chicago author-date
- Lan, Feng, Nan Zhang, Gabriële Holtappels, Natalie De Ruyck, Olga Krysko, Koen Van Crombruggen, Harald Braun, et al. 2018. “Staphylococcus Aureus Induces a Mucosal Type 2 Immune Response via Epithelial Cell-Derived Cytokines.” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 198 (4): 452–63. https://doi.org/10.1164/rccm.201710-2112oc.
- Chicago author-date (all authors)
- Lan, Feng, Nan Zhang, Gabriële Holtappels, Natalie De Ruyck, Olga Krysko, Koen Van Crombruggen, Harald Braun, Sebastian L Johnston, Nikos G Papadopoulos, Luo Zhang, and Claus Bachert. 2018. “Staphylococcus Aureus Induces a Mucosal Type 2 Immune Response via Epithelial Cell-Derived Cytokines.” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE 198 (4): 452–463. doi:10.1164/rccm.201710-2112oc.
- Vancouver
- 1.Lan F, Zhang N, Holtappels G, De Ruyck N, Krysko O, Van Crombruggen K, et al. Staphylococcus aureus induces a mucosal type 2 immune response via epithelial cell-derived cytokines. AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE. 2018;198(4):452–63.
- IEEE
- [1]F. Lan et al., “Staphylococcus aureus induces a mucosal type 2 immune response via epithelial cell-derived cytokines,” AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, vol. 198, no. 4, pp. 452–463, 2018.
@article{8582199, abstract = {{Rationale: Chronic rhinosinusitis with nasal polyps is characterized by a T-helper cell type 2-skewed upper airway inflammation. Mucosal Staphylococcus aureus colonization is found in the majority of patients with nasal polyps. S. aureus is known to induce type 2 cytokine release via enterotoxins. Objectives: To investigate the impact of non-enterotoxin-producing S. aureus on type 2 cytokine release. Methods: TSLP (thymic stromal lymphopoietin), IL-33, and type 2 cytokines were assessed in a human mucosal tissue model upon S. aureus infection. Measurements and Main Results: S. aureus exposure increased the expression of IL-33, TSLP, IL-5, and IL-13 in nasal polyp tissue, accompanied by elevated expression levels of TSLP and IL-33 receptors, predominantly on CD3(+) T cells. S. aureus infection led to the release of TSLP, but not IL-33, IL-5, or IL-13, from healthy inferior turbinate tissue. In contrast, S. epidermidis did not induce any epithelial cell-derived cytokines in nasal polyp or healthy tissue. S. aureus infection also increased the release of IL-33 and TSLP in BEAS-2B epithelial cells, accompanied by activation of NF-kappa B (nuclear factor kB) pathways. Incubation with CU-CPT22, a specific Toll-like receptor 2 antagonist, significantly reduced the S. aureus-induced release of TSLP and IL-33, and the activity of theNF-kappa B signal in BEAS-2B cells. Conclusions: This study demonstrates for the first time that S. aureus can directly induce epithelial cell-derived cytokine release via binding to Toll-like receptor 2, and may thereby propagate type 2 cytokine expression in nasal polyp tissue.}}, author = {{Lan, Feng and Zhang, Nan and Holtappels, Gabriële and De Ruyck, Natalie and Krysko, Olga and Van Crombruggen, Koen and Braun, Harald and Johnston, Sebastian L and Papadopoulos, Nikos G and Zhang, Luo and Bachert, Claus}}, issn = {{1073-449X}}, journal = {{AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE}}, keywords = {{chronic rhinosinusitis with nasal polyps,IL-33,Staphylococcus aureus,Th2,thymic stromal lymphopoietin,THYMIC STROMAL LYMPHOPOIETIN,NASAL POLYP DISEASE,CHRONIC RHINOSINUSITIS,HUMAN KERATINOCYTES,ENTEROTOXIN-B,INFLAMMATION,ASTHMA,IL-33,PROTEIN,TSLP}}, language = {{eng}}, number = {{4}}, pages = {{452--463}}, title = {{Staphylococcus aureus induces a mucosal type 2 immune response via epithelial cell-derived cytokines}}, url = {{http://dx.doi.org/10.1164/rccm.201710-2112oc}}, volume = {{198}}, year = {{2018}}, }
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