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Transport of neutral IgG2 versus anionic IgG4 in PD : implications on the electrokinetic model

Anneleen Pletinck (UGent) , Wim Van Biesen (UGent) , Clement Dequidt (UGent) and Sunny Eloot (UGent)
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Abstract
Background: It is debated whether transperitoneal membrane transport of larger (charged) molecules in peritoneal dialysis can be partially governed by the electrokinetic model. In this model, it is postulated that streaming potentials are generated across the capillary wall by forced filtration of an ionic solution, for example transcapillary ultrafiltration induced by osmotic forces as in peritoneal dialysis. We investigated the presence of streaming potentials in the process of transperitoneal transport in Peritoneal Dialysis (PD) patients by measuring ratios of dialysate concentrations of IgG2 (neutral) and IgG4 (negative), both 150kD, under different conditions of transcapillary ultrafiltration. Methods: Adult PD patients randomly got two consecutive dwells of 120 min each, with either 2 L Physioneal 1.36% or 3.86% glucose dialysis fluid (Baxter, USA) as their first dwell. A blood sample was taken at the test start, and dialysate samples were taken at 5, 15, 30, 60 and 120 min. IgG2 and IgG4 concentrations were measured (ELISA) and ratios calculated. Results: In 10 patients (65 +/- 17 years, 2017 months on dialysis), drained volume after 120 min was different between the 1.36% (1950 [1910; 2020] mL) and 3.86% (2540 [2380; 2800] mL) glucose dwells (P = 0.007). At none of the time points and irrespective of glucose concentration, a significant difference was found between the IgG2/IgG4 ratios at any time point. Conclusion: Our data failed to demonstrate a difference in the transport ratios of two macromolecules with same molecular weight but different charge, as would be expected by the electrokinetic model, and this despite sufficient differences in transcapillary ultrafiltration.
Keywords
PERITONEAL-DIALYSIS, ELECTRICAL-FIELD, PORE THEORY, PERMEABILITY, CAPD, PROTEINS, CHARGE, MEMBRANE, Peritoneal Dialysis, Transperitoneal membrane transport, Immunoglobulin, Three pore theory, Elektrokinetic model

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Please use this url to cite or link to this publication:

Chicago
Pletinck, Anneleen, Wim Van Biesen, Clement Dequidt, and Sunny Eloot. 2018. “Transport of Neutral IgG2 Versus Anionic IgG4 in PD : Implications on the Electrokinetic Model.” Bmc Nephrology 19.
APA
Pletinck, A., Van Biesen, W., Dequidt, C., & Eloot, S. (2018). Transport of neutral IgG2 versus anionic IgG4 in PD : implications on the electrokinetic model. BMC NEPHROLOGY, 19.
Vancouver
1.
Pletinck A, Van Biesen W, Dequidt C, Eloot S. Transport of neutral IgG2 versus anionic IgG4 in PD : implications on the electrokinetic model. BMC NEPHROLOGY. 2018;19.
MLA
Pletinck, Anneleen, Wim Van Biesen, Clement Dequidt, et al. “Transport of Neutral IgG2 Versus Anionic IgG4 in PD : Implications on the Electrokinetic Model.” BMC NEPHROLOGY 19 (2018): n. pag. Print.
@article{8581906,
  abstract     = {Background: It is debated whether transperitoneal membrane transport of larger (charged) molecules in peritoneal dialysis can be partially governed by the electrokinetic model. In this model, it is postulated that streaming potentials are generated across the capillary wall by forced filtration of an ionic solution, for example transcapillary ultrafiltration induced by osmotic forces as in peritoneal dialysis. We investigated the presence of streaming potentials in the process of transperitoneal transport in Peritoneal Dialysis (PD) patients by measuring ratios of dialysate concentrations of IgG2 (neutral) and IgG4 (negative), both 150kD, under different conditions of transcapillary ultrafiltration. 
Methods: Adult PD patients randomly got two consecutive dwells of 120 min each, with either 2 L Physioneal 1.36% or 3.86% glucose dialysis fluid (Baxter, USA) as their first dwell. A blood sample was taken at the test start, and dialysate samples were taken at 5, 15, 30, 60 and 120 min. IgG2 and IgG4 concentrations were measured (ELISA) and ratios calculated. 
Results: In 10 patients (65 +/- 17 years, 2017 months on dialysis), drained volume after 120 min was different between the 1.36% (1950 [1910; 2020] mL) and 3.86% (2540 [2380; 2800] mL) glucose dwells (P = 0.007). At none of the time points and irrespective of glucose concentration, a significant difference was found between the IgG2/IgG4 ratios at any time point. 
Conclusion: Our data failed to demonstrate a difference in the transport ratios of two macromolecules with same molecular weight but different charge, as would be expected by the electrokinetic model, and this despite sufficient differences in transcapillary ultrafiltration.},
  articleno    = {299},
  author       = {Pletinck, Anneleen and Van Biesen, Wim and Dequidt, Clement and Eloot, Sunny},
  issn         = {1471-2369},
  journal      = {BMC NEPHROLOGY},
  keywords     = {PERITONEAL-DIALYSIS,ELECTRICAL-FIELD,PORE THEORY,PERMEABILITY,CAPD,PROTEINS,CHARGE,MEMBRANE,Peritoneal Dialysis,Transperitoneal membrane transport,Immunoglobulin,Three pore theory,Elektrokinetic model},
  language     = {eng},
  pages        = {9},
  title        = {Transport of neutral IgG2 versus anionic IgG4 in PD : implications on the electrokinetic model},
  url          = {http://dx.doi.org/10.1186/s12882-018-1104-1},
  volume       = {19},
  year         = {2018},
}

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