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Distinct branches of the N-end rule pathway modulate the plant immune response

(2019) NEW PHYTOLOGIST. 221(2). p.988-1000
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Abstract
The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N-end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection. We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. In Arabidopsis thaliana Gln-specific amino-terminal (Nt)-amidase (NTAQ1) controls the expression of specific defence-response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (Pst). The Nt-Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen-induced stomatal closure. Transgenic barley with reduced HvPRT6 expression showed enhanced resistance to Ps. japonica and Blumeria graminis f. sp. hordei, indicating a conserved role of the pathway. We propose that that separate branches of the N-end rule pathway act as distinct components of the plant immune response in flowering plants.
Keywords
amino-terminal glutamine amidase, Group VII Ethylene Response Factor transcription factor, N-end rule pathway, plant immunity, proteostasis, NITRIC-OXIDE, ARABIDOPSIS-THALIANA, CAMALEXIN BIOSYNTHESIS, INDOLE-3-CARBOXYLIC ACID, HYPERSENSITIVE RESPONSE, DEFENSE RESPONSES, NITRATE REDUCTASE, DEFICIENT MUTANTS, SALICYLIC-ACID, BASAL DEFENSE

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Chicago
Vicente, Jorge, Guillermina M Mendiondo, Jarne Pauwels, Victoria Pastor, Yovanny Izquierdo, Christin Naumann, Mahsa Movahedi, et al. 2019. “Distinct Branches of the N-end Rule Pathway Modulate the Plant Immune Response.” New Phytologist 221 (2): 988–1000.
APA
Vicente, J., Mendiondo, G. M., Pauwels, J., Pastor, V., Izquierdo, Y., Naumann, C., Movahedi, M., et al. (2019). Distinct branches of the N-end rule pathway modulate the plant immune response. NEW PHYTOLOGIST, 221(2), 988–1000.
Vancouver
1.
Vicente J, Mendiondo GM, Pauwels J, Pastor V, Izquierdo Y, Naumann C, et al. Distinct branches of the N-end rule pathway modulate the plant immune response. NEW PHYTOLOGIST. 2019;221(2):988–1000.
MLA
Vicente, Jorge, Guillermina M Mendiondo, Jarne Pauwels, et al. “Distinct Branches of the N-end Rule Pathway Modulate the Plant Immune Response.” NEW PHYTOLOGIST 221.2 (2019): 988–1000. Print.
@article{8581641,
  abstract     = {The N-end rule pathway is a highly conserved constituent of the ubiquitin proteasome system, yet little is known about its biological roles. Here we explored the role of the N-end rule pathway in the plant immune response. We investigated the genetic influences of components of the pathway and known protein substrates on physiological, biochemical and metabolic responses to pathogen infection. We show that the glutamine (Gln) deamidation and cysteine (Cys) oxidation branches are both components of the plant immune system, through the E3 ligase PROTEOLYSIS (PRT)6. In Arabidopsis thaliana Gln-specific amino-terminal (Nt)-amidase (NTAQ1) controls the expression of specific defence-response genes, activates the synthesis pathway for the phytoalexin camalexin and influences basal resistance to the hemibiotroph pathogen Pseudomonas syringae pv tomato (Pst). The Nt-Cys ETHYLENE RESPONSE FACTOR VII transcription factor substrates enhance pathogen-induced stomatal closure. Transgenic barley with reduced HvPRT6 expression showed enhanced resistance to Ps. japonica and Blumeria graminis f. sp. hordei, indicating a conserved role of the pathway. We propose that that separate branches of the N-end rule pathway act as distinct components of the plant immune response in flowering plants.},
  author       = {Vicente, Jorge and Mendiondo, Guillermina M and Pauwels, Jarne and Pastor, Victoria and Izquierdo, Yovanny and Naumann, Christin and Movahedi, Mahsa and Rooney, Daniel and Gibbs, Daniel J and Smart, Katherine and Bachmair, Andreas and Gray, Julie E and Dissmeyer, Nico and Castresana, Carmen and Ray, Rumiana V and Gevaert, Kris and Holdsworth, Michael J},
  issn         = {0028-646X},
  journal      = {NEW PHYTOLOGIST},
  language     = {eng},
  number       = {2},
  pages        = {988--1000},
  title        = {Distinct branches of the N-end rule pathway modulate the plant immune response},
  url          = {http://dx.doi.org/10.1111/nph.15387},
  volume       = {221},
  year         = {2019},
}

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