Advanced search
1 file | 2.37 MB

Forensic tri-allelic SNP genotyping using nanopore sequencing

Author
Organization
Abstract
The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel of 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per sequencing run. The sample set consisted of three single-source human genomic reference control DNA samples and two GEDNAP samples, simulating casework samples. The primers for the multiplex SNP-loci PCR were taken from a study which researched their value in a forensic setting using conventional single-base extension technology. Workflows for multiplexed Oxford Nanopore Technologies 1D and 1D(2) sequencing were developed that provide correct genotyping of most SNP loci. Loci that are problematic for nanopore sequencing were characterized. When such loci are avoided, nanopore sequencing of forensic tri-allelic SNPs is technically feasible.
Keywords
Forensic, SNP, Next generation sequencing, Oxford nanopore technologies, MinION, SINGLE NUCLEOTIDE POLYMORPHISMS, TANDEM REPEAT LOCI, IDENTIFICATION, UTILITY, ASSAY

Downloads

  • Cornelis - 2019 - Forensic nanopore SNPs .pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 2.37 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Cornelis, Senne, Yannick Gansemans, Ann-Sophie Vander Plaetsen, Jana Weymaere, Sander Willems, Dieter Deforce, and Filip Van Nieuwerburgh. 2019. “Forensic Tri-allelic SNP Genotyping Using Nanopore Sequencing.” Forensic Science International-genetics 38: 204–210.
APA
Cornelis, S., Gansemans, Y., Vander Plaetsen, A.-S., Weymaere, J., Willems, S., Deforce, D., & Van Nieuwerburgh, F. (2019). Forensic tri-allelic SNP genotyping using nanopore sequencing. FORENSIC SCIENCE INTERNATIONAL-GENETICS, 38, 204–210.
Vancouver
1.
Cornelis S, Gansemans Y, Vander Plaetsen A-S, Weymaere J, Willems S, Deforce D, et al. Forensic tri-allelic SNP genotyping using nanopore sequencing. FORENSIC SCIENCE INTERNATIONAL-GENETICS. 2019;38:204–10.
MLA
Cornelis, Senne et al. “Forensic Tri-allelic SNP Genotyping Using Nanopore Sequencing.” FORENSIC SCIENCE INTERNATIONAL-GENETICS 38 (2019): 204–210. Print.
@article{8581454,
  abstract     = {The potential and current state-of-the-art of forensic SNP genotyping using nanopore sequencing was investigated with a panel of 16 tri-allelic single nucleotide polymorphisms (SNPs), multiplexing five samples per sequencing run. The sample set consisted of three single-source human genomic reference control DNA samples and two GEDNAP samples, simulating casework samples. The primers for the multiplex SNP-loci PCR were taken from a study which researched their value in a forensic setting using conventional single-base extension technology. Workflows for multiplexed Oxford Nanopore Technologies 1D and 1D(2) sequencing were developed that provide correct genotyping of most SNP loci. Loci that are problematic for nanopore sequencing were characterized. When such loci are avoided, nanopore sequencing of forensic tri-allelic SNPs is technically feasible.},
  author       = {Cornelis, Senne and Gansemans, Yannick and Vander Plaetsen, Ann-Sophie and Weymaere, Jana and Willems, Sander and Deforce, Dieter and Van Nieuwerburgh, Filip},
  issn         = {1872-4973},
  journal      = {FORENSIC SCIENCE INTERNATIONAL-GENETICS},
  language     = {eng},
  pages        = {204--210},
  title        = {Forensic tri-allelic SNP genotyping using nanopore sequencing},
  url          = {http://dx.doi.org/10.1016/j.fsigen.2018.11.012},
  volume       = {38},
  year         = {2019},
}

Altmetric
View in Altmetric
Web of Science
Times cited: