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Platelet-activating factor acetylhydrolase 1B3 (PAFAH1 B3) is required for the formation of the meiotic spindle during in vitro oocyte maturation

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Abstract
Platelet-activating factor (PAF) is a well-described autocrine growth factor involved in several reproductive processes and is tightly regulated by its hydrolysing enzyme, PAF acetylhydrolase 1B (PAFAHIB). This intracellular enzyme consists of three subunits: one regulatory, 1B1, and two catalytic, 1B2 and 1B3. PAFAHIB3 has remained uncharacterised until now. Here, we report that PAFAH1B3 is present during the different stages of the first meiotic division in bovine, murine and human oocytes. In these species, the PAFAH1B3 subunit was clearly present in the germinal vesicle, while at metaphase I and II, it localised primarily at the meiotic spindle structure. In cattle, manipulation of the microtubules of the spindle by nocodazole, taxol or cryopreservation revealed a close association with PAFAH1B3. On the other hand, disruption of the enzyme activity either by P11, a selective inhibitor of PAFAID B3, or by PAFAID B3 antibody microinjection, caused arrest at the MI stage with defective spindle morphology and consequent failure of first polar body extrusion. In conclusion, our results show that one of the catalytic subunits of PAFAH1B, namely PAFAH1B3, is present in bovine, murine and human oocytes and that it plays a functional role in spindle formation and meiotic progression during bovine oocyte maturation.
Keywords
alpha-tubulin, assisted reproduction, maturation failure, meiosis, platelet-activating factor, HUMAN FOLLICULAR-FLUID, CYTOPLASMIC DYNEIN, BOVINE OOCYTES, MOUSE OOCYTE, EMBRYO DEVELOPMENT, LIS1, MICROTUBULE, MEIOSIS, FERTILIZATION, METAPHASE

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Citation

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Chicago
Vandenberghe, Lynn, Björn Heindryckx, Katrien Smits, Katarzyna Szymanska-Vandendriessche, Nerea Ortiz Escribano, Minerva Ferrer Buitrago, Krishna Pavani, et al. 2018. “Platelet-activating Factor Acetylhydrolase 1B3 (PAFAH1 B3) Is Required for the Formation of the Meiotic Spindle During in Vitro Oocyte Maturation.” Reproduction Fertility and Development 30 (12): 1739–1750.
APA
Vandenberghe, Lynn, Heindryckx, B., Smits, K., Szymanska-Vandendriessche, K., Ortiz Escribano, N., Ferrer Buitrago, M., Pavani, K., et al. (2018). Platelet-activating factor acetylhydrolase 1B3 (PAFAH1 B3) is required for the formation of the meiotic spindle during in vitro oocyte maturation. REPRODUCTION FERTILITY AND DEVELOPMENT, 30(12), 1739–1750.
Vancouver
1.
Vandenberghe L, Heindryckx B, Smits K, Szymanska-Vandendriessche K, Ortiz Escribano N, Ferrer Buitrago M, et al. Platelet-activating factor acetylhydrolase 1B3 (PAFAH1 B3) is required for the formation of the meiotic spindle during in vitro oocyte maturation. REPRODUCTION FERTILITY AND DEVELOPMENT. 2018;30(12):1739–50.
MLA
Vandenberghe, Lynn, Björn Heindryckx, Katrien Smits, et al. “Platelet-activating Factor Acetylhydrolase 1B3 (PAFAH1 B3) Is Required for the Formation of the Meiotic Spindle During in Vitro Oocyte Maturation.” REPRODUCTION FERTILITY AND DEVELOPMENT 30.12 (2018): 1739–1750. Print.
@article{8580871,
  abstract     = {Platelet-activating factor (PAF) is a well-described autocrine growth factor involved in several reproductive processes and is tightly regulated by its hydrolysing enzyme, PAF acetylhydrolase 1B (PAFAHIB). This intracellular enzyme consists of three subunits: one regulatory, 1B1, and two catalytic, 1B2 and 1B3. PAFAHIB3 has remained uncharacterised until now. Here, we report that PAFAH1B3 is present during the different stages of the first meiotic division in bovine, murine and human oocytes. In these species, the PAFAH1B3 subunit was clearly present in the germinal vesicle, while at metaphase I and II, it localised primarily at the meiotic spindle structure. In cattle, manipulation of the microtubules of the spindle by nocodazole, taxol or cryopreservation revealed a close association with PAFAH1B3. On the other hand, disruption of the enzyme activity either by P11, a selective inhibitor of PAFAID B3, or by PAFAID B3 antibody microinjection, caused arrest at the MI stage with defective spindle morphology and consequent failure of first polar body extrusion. In conclusion, our results show that one of the catalytic subunits of PAFAH1B, namely PAFAH1B3, is present in bovine, murine and human oocytes and that it plays a functional role in spindle formation and meiotic progression during bovine oocyte maturation.},
  author       = {Vandenberghe, Lynn and Heindryckx, Bj{\"o}rn and Smits, Katrien and Szyma\'{n}ska-Vandendriessche, Katarzyna and Ortiz Escribano, Nerea and Ferrer Buitrago, Minerva and Pavani, Krishna and Peelman, Luc and Deforce, Dieter and De Sutter, Petra and Van Soom, Ann and De Schauwer, Catharina},
  issn         = {1031-3613},
  journal      = {REPRODUCTION FERTILITY AND DEVELOPMENT},
  language     = {eng},
  number       = {12},
  pages        = {1739--1750},
  title        = {Platelet-activating factor acetylhydrolase 1B3 (PAFAH1 B3) is required for the formation of the meiotic spindle during in vitro oocyte maturation},
  url          = {http://dx.doi.org/10.1071/rd18019},
  volume       = {30},
  year         = {2018},
}

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