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Live-cell imaging by confocal Raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in HeLa cells

Anatolii Abalymov, Roman A Verkhovskii, Marina V Novoselova, Bogdan Parakhonskiy (UGent) , Dmitry A Gorin, Alexey M Yashchenok and Gleb B Sukhorukov
(2018) BIOTECHNOLOGY JOURNAL. 13(11).
Author
Organization
Abstract
Porous calcium carbonate (CaCO3) vaterite particles are very attractive templates for the encapsulation of pharmaceuticals and for the construction of hollow polyelectrolyte capsules, sensors, and enzyme‐catalyzed reactors. Although CaCO3 is biocompatible and biodegradable, little is known about the intercellular behavior and properties of vaterite particles in the cytoplasm of cells. In this work, the authors combine confocal Raman and fluorescent microscopy for the imaging of porous CaCO3 vaterite particles in HeLa cells to study the uptake and status of the particles inside the cells in real time. Analysis of the fluorescence images shows that the particles penetrated the plasma membrane 3 h after being added to the cell culture and that the internalization of the particles continued up to 48 h. The crystal structure of individual vaterite particles in the cytoplasm of HeLa cells did not obviously change for 144 h. For clusters of particles, however, the authors identify Raman spectroscopic signatures of the stable calcite phase after 72 h of incubation, confirming an ion‐exchange mechanism of vaterite transformation to calcite. The results indicate that these imaging approach to examining inorganic particles in living cells may have theranostic applications.
Keywords
calcium carbonate, fluorescence microscopy, HeLa cells, Raman imaging, vaterite, POLYELECTROLYTE MULTILAYER CAPSULES, CACO3 PARTICLES, MAGNESIUM-ION, TRANSFORMATION MECHANISM, DRUG-DELIVERY, METAL-IONS, MICROCAPSULES, NANOPARTICLES, ENCAPSULATION, VATERITE

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Citation

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MLA
Abalymov, Anatolii, et al. “Live-Cell Imaging by Confocal Raman and Fluorescence Microscopy Recognizes the Crystal Structure of Calcium Carbonate Particles in HeLa Cells.” BIOTECHNOLOGY JOURNAL, vol. 13, no. 11, 2018, doi:10.1002/biot.201800071.
APA
Abalymov, A., Verkhovskii, R. A., Novoselova, M. V., Parakhonskiy, B., Gorin, D. A., Yashchenok, A. M., & Sukhorukov, G. B. (2018). Live-cell imaging by confocal Raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in HeLa cells. BIOTECHNOLOGY JOURNAL, 13(11). https://doi.org/10.1002/biot.201800071
Chicago author-date
Abalymov, Anatolii, Roman A Verkhovskii, Marina V Novoselova, Bogdan Parakhonskiy, Dmitry A Gorin, Alexey M Yashchenok, and Gleb B Sukhorukov. 2018. “Live-Cell Imaging by Confocal Raman and Fluorescence Microscopy Recognizes the Crystal Structure of Calcium Carbonate Particles in HeLa Cells.” BIOTECHNOLOGY JOURNAL 13 (11). https://doi.org/10.1002/biot.201800071.
Chicago author-date (all authors)
Abalymov, Anatolii, Roman A Verkhovskii, Marina V Novoselova, Bogdan Parakhonskiy, Dmitry A Gorin, Alexey M Yashchenok, and Gleb B Sukhorukov. 2018. “Live-Cell Imaging by Confocal Raman and Fluorescence Microscopy Recognizes the Crystal Structure of Calcium Carbonate Particles in HeLa Cells.” BIOTECHNOLOGY JOURNAL 13 (11). doi:10.1002/biot.201800071.
Vancouver
1.
Abalymov A, Verkhovskii RA, Novoselova MV, Parakhonskiy B, Gorin DA, Yashchenok AM, et al. Live-cell imaging by confocal Raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in HeLa cells. BIOTECHNOLOGY JOURNAL. 2018;13(11).
IEEE
[1]
A. Abalymov et al., “Live-cell imaging by confocal Raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in HeLa cells,” BIOTECHNOLOGY JOURNAL, vol. 13, no. 11, 2018.
@article{8579515,
  abstract     = {{Porous calcium carbonate (CaCO3) vaterite particles are very attractive templates for the encapsulation of pharmaceuticals and for the construction of hollow polyelectrolyte capsules, sensors, and enzyme‐catalyzed reactors. Although CaCO3 is biocompatible and biodegradable, little is known about the intercellular behavior and properties of vaterite particles in the cytoplasm of cells. In this work, the authors combine confocal Raman and fluorescent microscopy for the imaging of porous CaCO3 vaterite particles in HeLa cells to study the uptake and status of the particles inside the cells in real time. Analysis of the fluorescence images shows that the particles penetrated the plasma membrane 3 h after being added to the cell culture and that the internalization of the particles continued up to 48 h. The crystal structure of individual vaterite particles in the cytoplasm of HeLa cells did not obviously change for 144 h. For clusters of particles, however, the authors identify Raman spectroscopic signatures of the stable calcite phase after 72 h of incubation, confirming an ion‐exchange mechanism of vaterite transformation to calcite. The results indicate that these imaging approach to examining inorganic particles in living cells may have theranostic applications.}},
  articleno    = {{1800071}},
  author       = {{Abalymov, Anatolii and Verkhovskii, Roman A and Novoselova, Marina V and Parakhonskiy, Bogdan and Gorin, Dmitry A and Yashchenok, Alexey M and Sukhorukov, Gleb B}},
  issn         = {{1860-6768}},
  journal      = {{BIOTECHNOLOGY JOURNAL}},
  keywords     = {{calcium carbonate,fluorescence microscopy,HeLa cells,Raman imaging,vaterite,POLYELECTROLYTE MULTILAYER CAPSULES,CACO3 PARTICLES,MAGNESIUM-ION,TRANSFORMATION MECHANISM,DRUG-DELIVERY,METAL-IONS,MICROCAPSULES,NANOPARTICLES,ENCAPSULATION,VATERITE}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{8}},
  title        = {{Live-cell imaging by confocal Raman and fluorescence microscopy recognizes the crystal structure of calcium carbonate particles in HeLa cells}},
  url          = {{http://doi.org/10.1002/biot.201800071}},
  volume       = {{13}},
  year         = {{2018}},
}
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