Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis
- Author
- Robin Boiy (UGent) , Jonas Steenbrugge (UGent) , Jan Van Deun (UGent) , An Hendrix (UGent) , Evelyne Meyer (UGent) and Olivier De Wever (UGent)
- Organization
- Abstract
- Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential experimental and methodological parameters in chemotactic invasion assays in vitro and spontaneous metastasis assays in vivo. Following the analysis of 130 recent (<5years) research papers, several shortcomings in reporting were identified. Therefore, we strongly argue to increase experimental rigor which should result in a significant improvement with respect to reproducibility of preclinical metastasis research.
- Keywords
- Reproducibility, Chemotactic invasion, Methodological rigor, Spontaneous metastasis assay, Benchmarking, Minimal information guidelines, BREAST-CANCER, BASEMENT-MEMBRANE, PRECLINICAL MODEL, TUMOR, MICROENVIRONMENT, INVASION, MOUSE, TRANSITION, CELLS
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Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8579388
- MLA
- Boiy, Robin, et al. “Transparent Reporting of Experimental Parameters in Assays Measuring Phenotypic Steps in Metastasis.” CLINICAL & EXPERIMENTAL METASTASIS, vol. 35, no. 8, 2018, pp. 715–25, doi:10.1007/s10585-018-9944-4.
- APA
- Boiy, R., Steenbrugge, J., Van Deun, J., Hendrix, A., Meyer, E., & De Wever, O. (2018). Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis. CLINICAL & EXPERIMENTAL METASTASIS, 35(8), 715–725. https://doi.org/10.1007/s10585-018-9944-4
- Chicago author-date
- Boiy, Robin, Jonas Steenbrugge, Jan Van Deun, An Hendrix, Evelyne Meyer, and Olivier De Wever. 2018. “Transparent Reporting of Experimental Parameters in Assays Measuring Phenotypic Steps in Metastasis.” CLINICAL & EXPERIMENTAL METASTASIS 35 (8): 715–25. https://doi.org/10.1007/s10585-018-9944-4.
- Chicago author-date (all authors)
- Boiy, Robin, Jonas Steenbrugge, Jan Van Deun, An Hendrix, Evelyne Meyer, and Olivier De Wever. 2018. “Transparent Reporting of Experimental Parameters in Assays Measuring Phenotypic Steps in Metastasis.” CLINICAL & EXPERIMENTAL METASTASIS 35 (8): 715–725. doi:10.1007/s10585-018-9944-4.
- Vancouver
- 1.Boiy R, Steenbrugge J, Van Deun J, Hendrix A, Meyer E, De Wever O. Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis. CLINICAL & EXPERIMENTAL METASTASIS. 2018;35(8):715–25.
- IEEE
- [1]R. Boiy, J. Steenbrugge, J. Van Deun, A. Hendrix, E. Meyer, and O. De Wever, “Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis,” CLINICAL & EXPERIMENTAL METASTASIS, vol. 35, no. 8, pp. 715–725, 2018.
@article{8579388,
abstract = {{Metastasis is key to cancer mortality. Understanding its biology is vital for developing strategies to prevent and treat metastasis. Phenotypic assays to either study metastasis or evaluate anti-metastatic drugs are widely used in preclinical research. This technical note discusses the adherence of reporting essential experimental and methodological parameters in chemotactic invasion assays in vitro and spontaneous metastasis assays in vivo. Following the analysis of 130 recent (<5years) research papers, several shortcomings in reporting were identified. Therefore, we strongly argue to increase experimental rigor which should result in a significant improvement with respect to reproducibility of preclinical metastasis research.}},
author = {{Boiy, Robin and Steenbrugge, Jonas and Van Deun, Jan and Hendrix, An and Meyer, Evelyne and De Wever, Olivier}},
issn = {{0262-0898}},
journal = {{CLINICAL & EXPERIMENTAL METASTASIS}},
keywords = {{Reproducibility,Chemotactic invasion,Methodological rigor,Spontaneous metastasis assay,Benchmarking,Minimal information guidelines,BREAST-CANCER,BASEMENT-MEMBRANE,PRECLINICAL MODEL,TUMOR,MICROENVIRONMENT,INVASION,MOUSE,TRANSITION,CELLS}},
language = {{eng}},
number = {{8}},
pages = {{715--725}},
title = {{Transparent reporting of experimental parameters in assays measuring phenotypic steps in metastasis}},
url = {{http://dx.doi.org/10.1007/s10585-018-9944-4}},
volume = {{35}},
year = {{2018}},
}
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