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A CARD9 founder mutation disrupts NF-κB signaling by inhibiting BCL10 and MALT1 recruitment and signalosome formation

Marieke De Bruyne (UGent) , Levi Hoste (UGent) , Delfien Bogaert (UGent) , Lien Van den Bossche, Simon Tavernier (UGent) , Eef Parthoens (UGent) , Mélanie Migaud, Deborah Konopnicki, Jean Cyr Yombi, Bart Lambrecht (UGent) , et al.
(2018) FRONTIERS IN IMMUNOLOGY. 9.
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Abstract
Background: Inherited CARD9 deficiency constitutes a primary immunodeficiency predisposing uniquely to chronic and invasive fungal infections. Certain mutations are shown to negatively impact CARD9 protein expression and/or NF-kappa B activation, but the underlying biochemical mechanism remains to be fully understood. Objectives: To investigate a possible founder origin of a known CARD9 R70W mutation in five families of Turkish origin. To explore the biochemical mechanism of immunodeficiency by R70W CARD9. Methods: We performed haplotype analysis using microsatellite markers and SNPs. We designed a model system exploiting a gain-of-function (GOF) CARD9 L213L1 mutant that triggers constitutive NE-kappa B activation, analogous to an oncogenic CARD11 mutant, to study NF-KB signaling and signalosome formation. We performed reporter assays, immunoprecipitation and confocal imaging on HEK cells overexpressing different CARD9 variants. Results: We identified a common haplotype, thus providing evidence for a common Turkish founder. CARD9 R70W failed to activate NF-kappa B and abrogated NF-kappa B activation by WT CARD9 and by GOF CARD9. Notably, R70W CARD9 also exerted negative effects on NF-kappa B activation by CARD10, CARD11, and CARD14. Consistent with the NF-kappa B results, the R70W mutation prevented GOF CARD9 to pull down the signalosome partner proteins BCL10 and MALT1. This reflected into drastic reduction of BCL10 filamentous assemblies in a cellular context. Indeed, structural analysis revealed that position R70 in CARD9 maps at the putative interface between successive CARD domains in CARD9 filaments. Conclusions: The R70W mutation in CARD9 prevents NF-kappa B activation by inhibiting productive interactions with downstream BCL10 and MALT1, necessary for assembly of the filamentous CARD9-BCL10-MALT1 signalosome.
Keywords
CARD9 deficiency, founder mutation, BCL10, MALT1, CBM complex, NF-kappa B, filament, signalosome, CONSANGUINEOUS MARRIAGES, FUNGAL-INFECTIONS, INBORN-ERRORS, DEFICIENCY, IMMUNITY, PROTEIN, MENINGOENCEPHALITIS, ACTIVATION, SYSTEM, FAMILY

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MLA
De Bruyne, Marieke, et al. “A CARD9 Founder Mutation Disrupts NF-ΚB Signaling by Inhibiting BCL10 and MALT1 Recruitment and Signalosome Formation.” FRONTIERS IN IMMUNOLOGY, vol. 9, 2018, doi:10.3389/fimmu.2018.02366.
APA
De Bruyne, M., Hoste, L., Bogaert, D., Van den Bossche, L., Tavernier, S., Parthoens, E., … Dullaers, M. (2018). A CARD9 founder mutation disrupts NF-κB signaling by inhibiting BCL10 and MALT1 recruitment and signalosome formation. FRONTIERS IN IMMUNOLOGY, 9. https://doi.org/10.3389/fimmu.2018.02366
Chicago author-date
De Bruyne, Marieke, Levi Hoste, Delfien Bogaert, Lien Van den Bossche, Simon Tavernier, Eef Parthoens, Mélanie Migaud, et al. 2018. “A CARD9 Founder Mutation Disrupts NF-ΚB Signaling by Inhibiting BCL10 and MALT1 Recruitment and Signalosome Formation.” FRONTIERS IN IMMUNOLOGY 9. https://doi.org/10.3389/fimmu.2018.02366.
Chicago author-date (all authors)
De Bruyne, Marieke, Levi Hoste, Delfien Bogaert, Lien Van den Bossche, Simon Tavernier, Eef Parthoens, Mélanie Migaud, Deborah Konopnicki, Jean Cyr Yombi, Bart Lambrecht, Sabine Van daele, Ana Alves de Medeiros, Lieve Brochez, Rudi Beyaert, Elfride De Baere, Anne Puel, Jean-Laurent Casanova, Jean-Christophe Goffard, Savvas Savvides, Filomeen Haerynck, Jens Staal, and Melissa Dullaers. 2018. “A CARD9 Founder Mutation Disrupts NF-ΚB Signaling by Inhibiting BCL10 and MALT1 Recruitment and Signalosome Formation.” FRONTIERS IN IMMUNOLOGY 9. doi:10.3389/fimmu.2018.02366.
Vancouver
1.
De Bruyne M, Hoste L, Bogaert D, Van den Bossche L, Tavernier S, Parthoens E, et al. A CARD9 founder mutation disrupts NF-κB signaling by inhibiting BCL10 and MALT1 recruitment and signalosome formation. FRONTIERS IN IMMUNOLOGY. 2018;9.
IEEE
[1]
M. De Bruyne et al., “A CARD9 founder mutation disrupts NF-κB signaling by inhibiting BCL10 and MALT1 recruitment and signalosome formation,” FRONTIERS IN IMMUNOLOGY, vol. 9, 2018.
@article{8579130,
  abstract     = {{Background: Inherited CARD9 deficiency constitutes a primary immunodeficiency predisposing uniquely to chronic and invasive fungal infections. Certain mutations are shown to negatively impact CARD9 protein expression and/or NF-kappa B activation, but the underlying biochemical mechanism remains to be fully understood. 
Objectives: To investigate a possible founder origin of a known CARD9 R70W mutation in five families of Turkish origin. To explore the biochemical mechanism of immunodeficiency by R70W CARD9. 
Methods: We performed haplotype analysis using microsatellite markers and SNPs. We designed a model system exploiting a gain-of-function (GOF) CARD9 L213L1 mutant that triggers constitutive NE-kappa B activation, analogous to an oncogenic CARD11 mutant, to study NF-KB signaling and signalosome formation. We performed reporter assays, immunoprecipitation and confocal imaging on HEK cells overexpressing different CARD9 variants. 
Results: We identified a common haplotype, thus providing evidence for a common Turkish founder. CARD9 R70W failed to activate NF-kappa B and abrogated NF-kappa B activation by WT CARD9 and by GOF CARD9. Notably, R70W CARD9 also exerted negative effects on NF-kappa B activation by CARD10, CARD11, and CARD14. Consistent with the NF-kappa B results, the R70W mutation prevented GOF CARD9 to pull down the signalosome partner proteins BCL10 and MALT1. This reflected into drastic reduction of BCL10 filamentous assemblies in a cellular context. Indeed, structural analysis revealed that position R70 in CARD9 maps at the putative interface between successive CARD domains in CARD9 filaments. 
Conclusions: The R70W mutation in CARD9 prevents NF-kappa B activation by inhibiting productive interactions with downstream BCL10 and MALT1, necessary for assembly of the filamentous CARD9-BCL10-MALT1 signalosome.}},
  articleno    = {{2366}},
  author       = {{De Bruyne, Marieke and Hoste, Levi and Bogaert, Delfien and Van den Bossche, Lien and Tavernier, Simon and Parthoens, Eef and Migaud, Mélanie and Konopnicki, Deborah and Yombi, Jean Cyr and Lambrecht, Bart and Van daele, Sabine and Alves de Medeiros, Ana and Brochez, Lieve and Beyaert, Rudi and De Baere, Elfride and Puel, Anne and Casanova, Jean-Laurent and Goffard, Jean-Christophe and Savvides, Savvas and Haerynck, Filomeen and Staal, Jens and Dullaers, Melissa}},
  issn         = {{1664-3224}},
  journal      = {{FRONTIERS IN IMMUNOLOGY}},
  keywords     = {{CARD9 deficiency,founder mutation,BCL10,MALT1,CBM complex,NF-kappa B,filament,signalosome,CONSANGUINEOUS MARRIAGES,FUNGAL-INFECTIONS,INBORN-ERRORS,DEFICIENCY,IMMUNITY,PROTEIN,MENINGOENCEPHALITIS,ACTIVATION,SYSTEM,FAMILY}},
  language     = {{eng}},
  pages        = {{13}},
  title        = {{A CARD9 founder mutation disrupts NF-κB signaling by inhibiting BCL10 and MALT1 recruitment and signalosome formation}},
  url          = {{http://doi.org/10.3389/fimmu.2018.02366}},
  volume       = {{9}},
  year         = {{2018}},
}
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