Advanced search
1 file | 3.14 MB Add to list

Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community

(2018) ENVIRONMENTAL MICROBIOLOGY. 20(9). p.3406-3426
Author
Organization
Abstract
The spatial organization of gut microorganisms is important with respect to their functional role in the gut ecosystem. Regional differences in the longitudinal and lateral direction are, however, not frequently studied, given the difficulty to sample these human gut regions in vivo. Particularly the insoluble food particle-associated microbiota is poorly studied. Therefore, the long-term effects of insoluble wheat bran supplementation on the composition and functionality of the gut microbial community derived from six individuals were explored in the Dietary Particle-Mucosal-Simulator of the Human Intestinal Microbial Ecosystem in vitro model. Wheat bran stimulated propionate and butyrate production and induced shifts in the luminal and mucosal microbial community composition. The insoluble wheat bran residue and the mucus layer were identified as crucial platforms in sustaining diversity by selectively enriching species, which are not thriving in the luminal environment, including Lactobacillus, Bifidobacterium and Dialister species, Roseburia faecis, Prevotella copri and Bacteroides ovatus. Despite the evident habitat preference, some parallels could be drawn between the enrichment of taxa on bran platforms and their stimulation in the luminal and mucosal communities. Removing wheat bran during the wash-out period reversed the functional effects and gave rise to a blooming of some taxa that are considered opportunistic pathogens.
Keywords
RIBOSOMAL-RNA SEQUENCES, HUMAN FECAL BACTERIA, CHAIN FATTY-ACIDS, HUMAN LARGE-BOWEL, RESISTANT STARCH, INTESTINAL MICROBIOTA, ELECTRON-MICROSCOPY, DIETARY-FIBERS, FERMENTATION, DIVERSITY

Downloads

  • (...).pdf
    • full text
    • |
    • UGent only
    • |
    • PDF
    • |
    • 3.14 MB

Citation

Please use this url to cite or link to this publication:

MLA
De Paepe, Kim, Joran Verspreet, Kristin Verbeke, et al. “Introducing Insoluble Wheat Bran as a Gut Microbiota Niche in an in Vitro Dynamic Gut Model Stimulates Propionate and Butyrate Production and Induces Colon Region Specific Shifts in the Luminal and Mucosal Microbial Community.” ENVIRONMENTAL MICROBIOLOGY 20.9 (2018): 3406–3426. Print.
APA
De Paepe, K., Verspreet, J., Verbeke, K., Raes, J., Courtin, C. M., & Van de Wiele, T. (2018). Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community. ENVIRONMENTAL MICROBIOLOGY, 20(9), 3406–3426.
Chicago author-date
De Paepe, Kim, Joran Verspreet, Kristin Verbeke, Jeroen Raes, Christophe M. Courtin, and Tom Van de Wiele. 2018. “Introducing Insoluble Wheat Bran as a Gut Microbiota Niche in an in Vitro Dynamic Gut Model Stimulates Propionate and Butyrate Production and Induces Colon Region Specific Shifts in the Luminal and Mucosal Microbial Community.” Environmental Microbiology 20 (9): 3406–3426.
Chicago author-date (all authors)
De Paepe, Kim, Joran Verspreet, Kristin Verbeke, Jeroen Raes, Christophe M. Courtin, and Tom Van de Wiele. 2018. “Introducing Insoluble Wheat Bran as a Gut Microbiota Niche in an in Vitro Dynamic Gut Model Stimulates Propionate and Butyrate Production and Induces Colon Region Specific Shifts in the Luminal and Mucosal Microbial Community.” Environmental Microbiology 20 (9): 3406–3426.
Vancouver
1.
De Paepe K, Verspreet J, Verbeke K, Raes J, Courtin CM, Van de Wiele T. Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community. ENVIRONMENTAL MICROBIOLOGY. 2018;20(9):3406–26.
IEEE
[1]
K. De Paepe, J. Verspreet, K. Verbeke, J. Raes, C. M. Courtin, and T. Van de Wiele, “Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community,” ENVIRONMENTAL MICROBIOLOGY, vol. 20, no. 9, pp. 3406–3426, 2018.
@article{8579079,
  abstract     = {The spatial organization of gut microorganisms is important with respect to their functional role in the gut ecosystem. Regional differences in the longitudinal and lateral direction are, however, not frequently studied, given the difficulty to sample these human gut regions in vivo. Particularly the insoluble food particle-associated microbiota is poorly studied. Therefore, the long-term effects of insoluble wheat bran supplementation on the composition and functionality of the gut microbial community derived from six individuals were explored in the Dietary Particle-Mucosal-Simulator of the Human Intestinal Microbial Ecosystem in vitro model. Wheat bran stimulated propionate and butyrate production and induced shifts in the luminal and mucosal microbial community composition. The insoluble wheat bran residue and the mucus layer were identified as crucial platforms in sustaining diversity by selectively enriching species, which are not thriving in the luminal environment, including Lactobacillus, Bifidobacterium and Dialister species, Roseburia faecis, Prevotella copri and Bacteroides ovatus. Despite the evident habitat preference, some parallels could be drawn between the enrichment of taxa on bran platforms and their stimulation in the luminal and mucosal communities. Removing wheat bran during the wash-out period reversed the functional effects and gave rise to a blooming of some taxa that are considered opportunistic pathogens.},
  author       = {De Paepe, Kim and Verspreet, Joran and Verbeke, Kristin and Raes, Jeroen and Courtin, Christophe M. and Van de Wiele, Tom},
  issn         = {1462-2912},
  journal      = {ENVIRONMENTAL MICROBIOLOGY},
  keywords     = {RIBOSOMAL-RNA SEQUENCES,HUMAN FECAL BACTERIA,CHAIN FATTY-ACIDS,HUMAN LARGE-BOWEL,RESISTANT STARCH,INTESTINAL MICROBIOTA,ELECTRON-MICROSCOPY,DIETARY-FIBERS,FERMENTATION,DIVERSITY},
  language     = {eng},
  number       = {9},
  pages        = {3406--3426},
  title        = {Introducing insoluble wheat bran as a gut microbiota niche in an in vitro dynamic gut model stimulates propionate and butyrate production and induces colon region specific shifts in the luminal and mucosal microbial community},
  url          = {http://dx.doi.org/10.1111/1462-2920.14381},
  volume       = {20},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: