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Pollen characteristics and stigma receptivity for Anemone coronaria L.

Emmy Dhooghe (UGent) , Dirk Reheul (UGent) , Thea Versluys (UGent) and Marie-Christine Van Labeke (UGent)
(2018) EUPHYTICA. 214(11).
Author
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Abstract
To obtain crossing products, an efficient pollination and subsequent fertilization is essential. This efficient pollination is achieved by pollen germination and tube growth. Here, these pollen characteristics of 2 genetically differentiating cultivars of Anemone coronaria L. were investigated in vitro. For the essential components boron, calcium and an osmoticant, only calcium showed to be crucial for pollen germination. Boron concentrations influenced the pollen tube length with a concentration of 100mgl(-1) H3BO3 resulting in the longest pollen tubes. For the osmoticant sucrose, a concentration of 100gl(-1) caused a significant positive effect on both pollen germination and pollen tube length for the 2 cultivars. The cultivars reacted similarly with respect to the investigated compounds. Next to this, the pollen development was correlated with 8 different flower stages in A. coronaria. As a final point, the germination of an optimized pollen germination medium was compared with in vivo pollen germination in cross-pollinations within the same cultivar (identified by aniline blue staining). For Mistral Wine', pollen germination percentage was lower in vitro than in vivo, while Wicabri Blue' pollen showed no significant difference in germination rates in vivo and in vitro. To achieve fertilization a following requisite is that the stigma is receptive. To study this, the most receptive female flower stage of the 8 different flower stages for A. coronaria was characterized by aniline blue staining. A. coronaria clearly showed protogyny.
Keywords
Aniline blue staining, In vitro, Microspore, Pollen germination, Pollen tube, Ranunculaceae, IN-VITRO, TUBE GROWTH, GENERATIVE NUCLEUS, NICOTIANA-TABACUM, GERMINATION, VIABILITY, CULTURE, RANUNCULACEAE, BROMELIACEAE, MORPHOLOGY

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MLA
Dhooghe, Emmy, et al. “Pollen Characteristics and Stigma Receptivity for Anemone Coronaria L.” EUPHYTICA, vol. 214, no. 11, 2018, doi:10.1007/s10681-018-2294-0.
APA
Dhooghe, E., Reheul, D., Versluys, T., & Van Labeke, M.-C. (2018). Pollen characteristics and stigma receptivity for Anemone coronaria L. EUPHYTICA, 214(11). https://doi.org/10.1007/s10681-018-2294-0
Chicago author-date
Dhooghe, Emmy, Dirk Reheul, Thea Versluys, and Marie-Christine Van Labeke. 2018. “Pollen Characteristics and Stigma Receptivity for Anemone Coronaria L.” EUPHYTICA 214 (11). https://doi.org/10.1007/s10681-018-2294-0.
Chicago author-date (all authors)
Dhooghe, Emmy, Dirk Reheul, Thea Versluys, and Marie-Christine Van Labeke. 2018. “Pollen Characteristics and Stigma Receptivity for Anemone Coronaria L.” EUPHYTICA 214 (11). doi:10.1007/s10681-018-2294-0.
Vancouver
1.
Dhooghe E, Reheul D, Versluys T, Van Labeke M-C. Pollen characteristics and stigma receptivity for Anemone coronaria L. EUPHYTICA. 2018;214(11).
IEEE
[1]
E. Dhooghe, D. Reheul, T. Versluys, and M.-C. Van Labeke, “Pollen characteristics and stigma receptivity for Anemone coronaria L.,” EUPHYTICA, vol. 214, no. 11, 2018.
@article{8578934,
  abstract     = {{To obtain crossing products, an efficient pollination and subsequent fertilization is essential. This efficient pollination is achieved by pollen germination and tube growth. Here, these pollen characteristics of 2 genetically differentiating cultivars of Anemone coronaria L. were investigated in vitro. For the essential components boron, calcium and an osmoticant, only calcium showed to be crucial for pollen germination. Boron concentrations influenced the pollen tube length with a concentration of 100mgl(-1) H3BO3 resulting in the longest pollen tubes. For the osmoticant sucrose, a concentration of 100gl(-1) caused a significant positive effect on both pollen germination and pollen tube length for the 2 cultivars. The cultivars reacted similarly with respect to the investigated compounds. Next to this, the pollen development was correlated with 8 different flower stages in A. coronaria. As a final point, the germination of an optimized pollen germination medium was compared with in vivo pollen germination in cross-pollinations within the same cultivar (identified by aniline blue staining). For Mistral Wine', pollen germination percentage was lower in vitro than in vivo, while Wicabri Blue' pollen showed no significant difference in germination rates in vivo and in vitro. To achieve fertilization a following requisite is that the stigma is receptive. To study this, the most receptive female flower stage of the 8 different flower stages for A. coronaria was characterized by aniline blue staining. A. coronaria clearly showed protogyny.}},
  articleno    = {{211}},
  author       = {{Dhooghe, Emmy and Reheul, Dirk and Versluys, Thea and Van Labeke, Marie-Christine}},
  issn         = {{0014-2336}},
  journal      = {{EUPHYTICA}},
  keywords     = {{Aniline blue staining,In vitro,Microspore,Pollen germination,Pollen tube,Ranunculaceae,IN-VITRO,TUBE GROWTH,GENERATIVE NUCLEUS,NICOTIANA-TABACUM,GERMINATION,VIABILITY,CULTURE,RANUNCULACEAE,BROMELIACEAE,MORPHOLOGY}},
  language     = {{eng}},
  number       = {{11}},
  pages        = {{13}},
  title        = {{Pollen characteristics and stigma receptivity for Anemone coronaria L.}},
  url          = {{http://dx.doi.org/10.1007/s10681-018-2294-0}},
  volume       = {{214}},
  year         = {{2018}},
}
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