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Multi-parametric screening in Arabidopsis thaliana seedlings

Long Nguyen (UGent) , Andrzej Drozdzecki (UGent) , Vera Goossens (UGent) , Bert De Rybel (UGent) , Tom Beeckman (UGent) and Dominique Audenaert (UGent)
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Abstract
Phenotypic screening and subsequent target identification approaches are very valuable to identify chemical probes that can be used to explore the connection between phenotypes and biological pathways. However, assessing a phenotypic effect in plants in a high-throughput fashion is a challenging task and often requires expensive readout devices. In this chapter, we describe a cost-effective multi-parametric screening procedure that is compatible with liquid-handling systems and that enables the assessment of phenotypes in Arabidopsis thaliana seedlings in an automated way.
Keywords
Arabidopsis thaliana, Automation, High-content screening, Multi-parametric analysis

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Please use this url to cite or link to this publication:

Chicago
Nguyen, Long, Andrzej Drozdzecki, Vera Goossens, Bert De Rybel, Tom Beeckman, and Dominique Audenaert. 2018. “Multi-parametric Screening in Arabidopsis Thaliana Seedlings.” In Plant Chemical Genomics : Methods and Protocols, ed. Friedrich Fauser and Martin Jonikas, 1795:1–7. New York, NY, USA: Springer Humana Press.
APA
Nguyen, Long, Drozdzecki, A., Goossens, V., De Rybel, B., Beeckman, T., & Audenaert, D. (2018). Multi-parametric screening in Arabidopsis thaliana seedlings. In F. Fauser & M. Jonikas (Eds.), Plant chemical genomics : methods and protocols (Vol. 1795, pp. 1–7). New York, NY, USA: Springer Humana Press.
Vancouver
1.
Nguyen L, Drozdzecki A, Goossens V, De Rybel B, Beeckman T, Audenaert D. Multi-parametric screening in Arabidopsis thaliana seedlings. In: Fauser F, Jonikas M, editors. Plant chemical genomics : methods and protocols. New York, NY, USA: Springer Humana Press; 2018. p. 1–7.
MLA
Nguyen, Long, Andrzej Drozdzecki, Vera Goossens, et al. “Multi-parametric Screening in Arabidopsis Thaliana Seedlings.” Plant Chemical Genomics : Methods and Protocols. Ed. Friedrich Fauser & Martin Jonikas. Vol. 1795. New York, NY, USA: Springer Humana Press, 2018. 1–7. Print.
@incollection{8577917,
  abstract     = {Phenotypic screening and subsequent target identification approaches are very valuable to identify chemical probes that can be used to explore the connection between phenotypes and biological pathways. However, assessing a phenotypic effect in plants in a high-throughput fashion is a challenging task and often requires expensive readout devices. In this chapter, we describe a cost-effective multi-parametric screening procedure that is compatible with liquid-handling systems and that enables the assessment of phenotypes in Arabidopsis thaliana seedlings in an automated way.},
  author       = {Nguyen, Long and Drozdzecki, Andrzej and Goossens, Vera and De Rybel, Bert and Beeckman, Tom and Audenaert, Dominique},
  booktitle    = {Plant chemical genomics : methods and protocols},
  editor       = {Fauser, Friedrich and Jonikas, Martin},
  isbn         = {9781493978731},
  issn         = {1064-3745},
  language     = {eng},
  pages        = {1--7},
  publisher    = {Springer Humana Press},
  series       = {Methods in Molecular Biology},
  title        = {Multi-parametric screening in Arabidopsis thaliana seedlings},
  url          = {http://dx.doi.org/10.1007/978-1-4939-7874-8\_1},
  volume       = {1795},
  year         = {2018},
}

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