Increasing uniformity of biosurfactant production in Starmerella bombicola via the expression of chimeric cytochrome P450s
- Author
- Robin Geys (UGent) , Marilyn De Graeve (UGent) , Sofie Lodens, Jeroen Van Malderen, Christophe Lemmens, Margaux De Smet, Stein Mincke (UGent) , Inge Van Bogaert (UGent) , Christian Stevens (UGent) , Sofie De Maeseneire (UGent) , Sophie Roelants (UGent) and Wim Soetaert (UGent)
- Organization
- Abstract
- Sophorolipids are one of the best known microbial biosurfactants and are produced by several yeast species. The best studied producer is Starmerella bombicola, a non-pathogenic yeast associated in nature with bumblebees. Sophorolipids are built up of the rare disaccharide sophorose, which is attached to a fatty acid through a glyosidic bound. Sophorolipids produced by S. bombicola mainly contain oleic acid as the incorporated hydrophobic group. Other chain lengths can, to a certain content, be incorporated by feeding the yeast with substrates of alternative chain lengths. However, the efficiency for such substrates is low as compared to the preferred C18 chain length and defined by the substrate specificity of the first enzymatic step in sophorolipid biosynthesis, i.e., the cytochrome P450 enzyme CYP52M1. To increase product uniformity and diversity at the same time, a new strain of S. bombicola was developed that produces sophorolipids with a palmitic acid acyl chain. This was achieved by heterologous expression of the cytochrome P450 cyp1 gene of Ustilago maydis and feeding with palmitic acid. Optimization of the production was done by protein and process engineering.
- Keywords
- sophorolipid, biosurfactant, Starmerella bombicola, cytochrome P450, chimeragenesis, enzyme engineering, process engineering, fermentation, purification, NMR, SOPHOROSE LIPID PRODUCTION, BIOSYNTHETIC GENE-CLUSTER, MICROBIAL-PRODUCTION, IDENTIFICATION, REGIOSELECTIVITY, SOPHOROLIPIDS, FERMENTATION, BM-3
Downloads
-
colloids-02-00042.pdf
- full text
- |
- open access
- |
- |
- 3.20 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8577712
- MLA
- Geys, Robin, et al. “Increasing Uniformity of Biosurfactant Production in Starmerella Bombicola via the Expression of Chimeric Cytochrome P450s.” COLLOIDS AND INTERFACES, vol. 2, no. 4, 2018, doi:10.3390/colloids2040042.
- APA
- Geys, R., De Graeve, M., Lodens, S., Van Malderen, J., Lemmens, C., De Smet, M., … Soetaert, W. (2018). Increasing uniformity of biosurfactant production in Starmerella bombicola via the expression of chimeric cytochrome P450s. COLLOIDS AND INTERFACES, 2(4). https://doi.org/10.3390/colloids2040042
- Chicago author-date
- Geys, Robin, Marilyn De Graeve, Sofie Lodens, Jeroen Van Malderen, Christophe Lemmens, Margaux De Smet, Stein Mincke, et al. 2018. “Increasing Uniformity of Biosurfactant Production in Starmerella Bombicola via the Expression of Chimeric Cytochrome P450s.” COLLOIDS AND INTERFACES 2 (4). https://doi.org/10.3390/colloids2040042.
- Chicago author-date (all authors)
- Geys, Robin, Marilyn De Graeve, Sofie Lodens, Jeroen Van Malderen, Christophe Lemmens, Margaux De Smet, Stein Mincke, Inge Van Bogaert, Christian Stevens, Sofie De Maeseneire, Sophie Roelants, and Wim Soetaert. 2018. “Increasing Uniformity of Biosurfactant Production in Starmerella Bombicola via the Expression of Chimeric Cytochrome P450s.” COLLOIDS AND INTERFACES 2 (4). doi:10.3390/colloids2040042.
- Vancouver
- 1.Geys R, De Graeve M, Lodens S, Van Malderen J, Lemmens C, De Smet M, et al. Increasing uniformity of biosurfactant production in Starmerella bombicola via the expression of chimeric cytochrome P450s. COLLOIDS AND INTERFACES. 2018;2(4).
- IEEE
- [1]R. Geys et al., “Increasing uniformity of biosurfactant production in Starmerella bombicola via the expression of chimeric cytochrome P450s,” COLLOIDS AND INTERFACES, vol. 2, no. 4, 2018.
@article{8577712,
abstract = {{Sophorolipids are one of the best known microbial biosurfactants and are produced by several yeast species. The best studied producer is Starmerella bombicola, a non-pathogenic yeast associated in nature with bumblebees. Sophorolipids are built up of the rare disaccharide sophorose, which is attached to a fatty acid through a glyosidic bound. Sophorolipids produced by S. bombicola mainly contain oleic acid as the incorporated hydrophobic group. Other chain lengths can, to a certain content, be incorporated by feeding the yeast with substrates of alternative chain lengths. However, the efficiency for such substrates is low as compared to the preferred C18 chain length and defined by the substrate specificity of the first enzymatic step in sophorolipid biosynthesis, i.e., the cytochrome P450 enzyme CYP52M1. To increase product uniformity and diversity at the same time, a new strain of S. bombicola was developed that produces sophorolipids with a palmitic acid acyl chain. This was achieved by heterologous expression of the cytochrome P450 cyp1 gene of Ustilago maydis and feeding with palmitic acid. Optimization of the production was done by protein and process engineering.}},
articleno = {{42}},
author = {{Geys, Robin and De Graeve, Marilyn and Lodens, Sofie and Van Malderen, Jeroen and Lemmens, Christophe and De Smet, Margaux and Mincke, Stein and Van Bogaert, Inge and Stevens, Christian and De Maeseneire, Sofie and Roelants, Sophie and Soetaert, Wim}},
issn = {{2504-5377}},
journal = {{COLLOIDS AND INTERFACES}},
keywords = {{sophorolipid,biosurfactant,Starmerella bombicola,cytochrome P450,chimeragenesis,enzyme engineering,process engineering,fermentation,purification,NMR,SOPHOROSE LIPID PRODUCTION,BIOSYNTHETIC GENE-CLUSTER,MICROBIAL-PRODUCTION,IDENTIFICATION,REGIOSELECTIVITY,SOPHOROLIPIDS,FERMENTATION,BM-3}},
language = {{eng}},
number = {{4}},
pages = {{13}},
title = {{Increasing uniformity of biosurfactant production in Starmerella bombicola via the expression of chimeric cytochrome P450s}},
url = {{http://doi.org/10.3390/colloids2040042}},
volume = {{2}},
year = {{2018}},
}
- Altmetric
- View in Altmetric