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Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site

Jens Staal (UGent) , Wouter De Schamphelaire (UGent) and Rudi Beyaert (UGent)
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Organization
Abstract
Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185 bp fully functional high-copy cloning plasmid with an extended multiple cloning site (MCS). To our knowledge, this is the smallest high-copy cloning vector ever described.
Keywords
PCR, recombination, cloning, engineering, biotechnology, synthetic biology, synthetic nucleotide, plasmids, repository, minimalism, Escherichia coli, mutagenesis

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Citation

Please use this url to cite or link to this publication:

MLA
Staal, Jens, Wouter De Schamphelaire, and Rudi Beyaert. “Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site.” PREPRINTS 2018 : n. pag. Print.
APA
Staal, J., De Schamphelaire, W., & Beyaert, R. (2018). Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site. PREPRINTS. Basel, Switzerland: MDPI.
Chicago author-date
Staal, Jens, Wouter De Schamphelaire, and Rudi Beyaert. 2018. “Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site.” Preprints. Basel, Switzerland: MDPI.
Chicago author-date (all authors)
Staal, Jens, Wouter De Schamphelaire, and Rudi Beyaert. 2018. “Engineering a Minimal Cloning Vector from a pUC18 Plasmid Backbone with an Extended Multiple Cloning Site.” Preprints. Basel, Switzerland: MDPI.
Vancouver
1.
Staal J, De Schamphelaire W, Beyaert R. Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site. PREPRINTS. Basel, Switzerland: MDPI; 2018.
IEEE
[1]
J. Staal, W. De Schamphelaire, and R. Beyaert, “Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site,” PREPRINTS. MDPI, Basel, Switzerland, 2018.
@misc{8577570,
  abstract     = {Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis methods. A small backbone also opens up for additional unique restriction enzyme cloning sites. Here we describe the generation of pICOz, a 1185 bp fully functional high-copy cloning plasmid with an extended multiple cloning site (MCS). To our knowledge, this is the smallest high-copy cloning vector ever described.},
  articleno    = {2018070287},
  author       = {Staal, Jens and De Schamphelaire, Wouter and Beyaert, Rudi},
  keywords     = {PCR,recombination,cloning,engineering,biotechnology,synthetic biology,synthetic nucleotide,plasmids,repository,minimalism,Escherichia coli,mutagenesis},
  language     = {eng},
  pages        = {9},
  publisher    = {MDPI},
  series       = {PREPRINTS},
  title        = {Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site},
  url          = {http://dx.doi.org/10.20944/preprints201807.0287.v2},
  year         = {2018},
}

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