Advanced search
1 file | 990.44 KB Add to list

A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement

Author
Organization
Abstract
Background: Systemic sclerosis (SSc) is a disorder characterized by immune system alterations, vasculopathy and fibrosis. SSc-related interstitial lung disease (ILD) represents a common and early complication, being the leading cause of mortality. Monocytes/macrophages seem to have a key role in SSc-related ILD. Interestingly, the classically (M1) and alternatively (M2) activated monocyte/macrophage phenotype categorization is currently under revision. Our aim was to evaluate if circulating monocyte/macrophage phenotype could be used as biomarker for lung involvement in SSc. To this purpose we developed a wide phenotype characterization of circulating monocyte/macrophage subsets in SSc patients and we evaluated possible relations with lung involvement parameter values. Methods: A single centre cross-sectional study was performed in fifty-five consecutive SSc patients, during the year 2017. All clinical and instrumental tests requested for SSc follow up and in particular, lung computed tomography (CT) scan, pulmonary function tests (PFTs), Doppler echocardiography with systolic pulmonary artery pressure (sPAP) measurement, blood pro-hormone of brain natriuretic peptide (pro-BNP) evaluation, were performed in each patient in a maximum one-month period. Flow cytometry characterization of circulating cells belonging to the monocyte/macrophage lineage was performed using specific M1 (CD80, CD86, TLR2 and TLR4) and M2 surface markers (CD204, CD163 and CD206). Non-parametric tests were used for statistical analysis. Results: A higher percentage of circulating CD204(+)CD163(+)CD206(+)TLR4(+)CD80(+)CD86(+) and CD14(+)CD206(+)CD163(+)CD204(+)TLR4(+)CD80(+)CD86(+) mixed M1/M2 monocyte/macrophage subsets, was identified to characterize patients affected by SSc-related ILD and higher systolic pulmonary artery pressure. Mixed M1/M2 monocyte/macrophage subset showed higher percentages in patients positive for anti-topoisomerase antibody, a known lung involvement predictor. Conclusions: The present study shows for the first time, through a wide flow cytometry surface marker analysis, that higher circulating mixed M1/M2 monocyte/macrophage cell percentages are associated with ILD, sPAP and anti-topoisomerase antibody positivity in SSc, opening the path for research on their possible role as pathogenic or biomarker elements for SSc lung involvement.
Keywords
Systemic sclerosis, Interstitial lung disease, Pulmonary artery hypertension, Monocyte/macrophage phenotype, M1, M2, Innate immunity, Lung CT scan, Pulmonary function tests, Flow cytometry, Anti-topoisomerase antibody, PULMONARY ARTERIAL-HYPERTENSION, ALTERNATIVELY ACTIVATED MACROPHAGES, DISEASE, MONOCYTES, FIBROSIS, POLARIZATION, INTERFERON, PREDICTION, SURVIVAL, CRITERIA

Downloads

  • Trombetta Smith Vanessa repiratory research 2018.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 990.44 KB

Citation

Please use this url to cite or link to this publication:

MLA
Trombetta, Amelia Chiara, Stefano Soldano, Paola Contini, et al. “A Circulating Cell Population Showing Both M1 and M2 Monocyte/macrophage Surface Markers Characterizes Systemic Sclerosis Patients with Lung Involvement.” RESPIRATORY RESEARCH 19 (2018): n. pag. Print.
APA
Trombetta, Amelia Chiara, Soldano, S., Contini, P., Tomatis, V., Ruaro, B., Paolino, S., Brizzolara, R., et al. (2018). A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement. RESPIRATORY RESEARCH, 19.
Chicago author-date
Trombetta, Amelia Chiara, Stefano Soldano, Paola Contini, Veronica Tomatis, Barbara Ruaro, Sabrina Paolino, Renata Brizzolara, et al. 2018. “A Circulating Cell Population Showing Both M1 and M2 Monocyte/macrophage Surface Markers Characterizes Systemic Sclerosis Patients with Lung Involvement.” Respiratory Research 19.
Chicago author-date (all authors)
Trombetta, Amelia Chiara, Stefano Soldano, Paola Contini, Veronica Tomatis, Barbara Ruaro, Sabrina Paolino, Renata Brizzolara, Paola Montagna, Alberto Sulli, Carmen Pizzorni, Vanessa Smith, and Maurizio Cutolo. 2018. “A Circulating Cell Population Showing Both M1 and M2 Monocyte/macrophage Surface Markers Characterizes Systemic Sclerosis Patients with Lung Involvement.” Respiratory Research 19.
Vancouver
1.
Trombetta AC, Soldano S, Contini P, Tomatis V, Ruaro B, Paolino S, et al. A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement. RESPIRATORY RESEARCH. 2018;19.
IEEE
[1]
A. C. Trombetta et al., “A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement,” RESPIRATORY RESEARCH, vol. 19, 2018.
@article{8576153,
  abstract     = {Background: Systemic sclerosis (SSc) is a disorder characterized by immune system alterations, vasculopathy and fibrosis. SSc-related interstitial lung disease (ILD) represents a common and early complication, being the leading cause of mortality. Monocytes/macrophages seem to have a key role in SSc-related ILD. Interestingly, the classically (M1) and alternatively (M2) activated monocyte/macrophage phenotype categorization is currently under revision. 
Our aim was to evaluate if circulating monocyte/macrophage phenotype could be used as biomarker for lung involvement in SSc. To this purpose we developed a wide phenotype characterization of circulating monocyte/macrophage subsets in SSc patients and we evaluated possible relations with lung involvement parameter values. 
Methods: A single centre cross-sectional study was performed in fifty-five consecutive SSc patients, during the year 2017. All clinical and instrumental tests requested for SSc follow up and in particular, lung computed tomography (CT) scan, pulmonary function tests (PFTs), Doppler echocardiography with systolic pulmonary artery pressure (sPAP) measurement, blood pro-hormone of brain natriuretic peptide (pro-BNP) evaluation, were performed in each patient in a maximum one-month period. Flow cytometry characterization of circulating cells belonging to the monocyte/macrophage lineage was performed using specific M1 (CD80, CD86, TLR2 and TLR4) and M2 surface markers (CD204, CD163 and CD206). Non-parametric tests were used for statistical analysis. 
Results: A higher percentage of circulating CD204(+)CD163(+)CD206(+)TLR4(+)CD80(+)CD86(+) and CD14(+)CD206(+)CD163(+)CD204(+)TLR4(+)CD80(+)CD86(+) mixed M1/M2 monocyte/macrophage subsets, was identified to characterize patients affected by SSc-related ILD and higher systolic pulmonary artery pressure. Mixed M1/M2 monocyte/macrophage subset showed higher percentages in patients positive for anti-topoisomerase antibody, a known lung involvement predictor. 
Conclusions: The present study shows for the first time, through a wide flow cytometry surface marker analysis, that higher circulating mixed M1/M2 monocyte/macrophage cell percentages are associated with ILD, sPAP and anti-topoisomerase antibody positivity in SSc, opening the path for research on their possible role as pathogenic or biomarker elements for SSc lung involvement.},
  articleno    = {186},
  author       = {Trombetta, Amelia Chiara and Soldano, Stefano and Contini, Paola and Tomatis, Veronica and Ruaro, Barbara and Paolino, Sabrina and Brizzolara, Renata and Montagna, Paola and Sulli, Alberto and Pizzorni, Carmen and Smith, Vanessa and Cutolo, Maurizio},
  issn         = {1465-993X},
  journal      = {RESPIRATORY RESEARCH},
  keywords     = {Systemic sclerosis,Interstitial lung disease,Pulmonary artery hypertension,Monocyte/macrophage phenotype,M1,M2,Innate immunity,Lung CT scan,Pulmonary function tests,Flow cytometry,Anti-topoisomerase antibody,PULMONARY ARTERIAL-HYPERTENSION,ALTERNATIVELY ACTIVATED MACROPHAGES,DISEASE,MONOCYTES,FIBROSIS,POLARIZATION,INTERFERON,PREDICTION,SURVIVAL,CRITERIA},
  language     = {eng},
  pages        = {12},
  title        = {A circulating cell population showing both M1 and M2 monocyte/macrophage surface markers characterizes systemic sclerosis patients with lung involvement},
  url          = {http://dx.doi.org/10.1186/s12931-018-0891-z},
  volume       = {19},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: