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Early mannitol-triggered changes in the Arabidopsis leaf (phospho) proteome reveal growth regulators

Natalia Nikonorova (UGent) , Lisa Van den Broeck (UGent) , Shanshuo Zhu (UGent) , Brigitte Van De Cotte (UGent) , Marieke Dubois (UGent) , Kris Gevaert (UGent) , Dirk Inzé (UGent) and Ive De Smet (UGent)
(2018) JOURNAL OF EXPERIMENTAL BOTANY. 69(19). p.4591-4607
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Abstract
Leaf growth is a complex, quantitative trait, controlled by a plethora of regulatory mechanisms. Diverse environmental stimuli inhibit leaf growth to cope with the perceived stress. In plant research, mannitol is often used to impose osmotic stress and study the underlying growth-repressing mechanisms. In growing leaf tissue of plants briefly exposed to mannitol-induced stress, a highly interconnected gene regulatory network is induced. However, early signalling and associated protein phosphorylation events that probably precede part of these transcriptional changes and that potentially act at the onset of mannitol-induced leaf size reduction are largely unknown. Here, we performed a proteome and phosphoproteome analysis on growing leaf tissue of Arabidopsis thaliana plants exposed to mild mannitol-induced stress and captured the fast (within the first half hour) events associated with this stress. Based on this in-depth data analysis, 167 and 172 differentially regulated proteins and phosphorylated sites were found. We provide these data sets as a community resource and we flag differentially phosphorylated proteins with described growth-regulatory functions, but we also illustrate potential novel regulators of shoot growth.
Keywords
MEMBRANE H+-ATPASE, SALT STRESS-RESPONSE, PLASMA-MEMBRANE, OSMOTIC-STRESS, PLANT DEVELOPMENT, ABIOTIC STRESSES, PHOSPHORYLATION, WATER, THALIANA, DROUGHT, AHA2, Arabidopsis thaliana, CRRSP38, leaf growth, mild osmotic stress, phosphoproteome, signalling

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Citation

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MLA
Nikonorova, Natalia, Lisa Van den Broeck, Shanshuo Zhu, et al. “Early Mannitol-triggered Changes in the Arabidopsis Leaf (phospho) Proteome Reveal Growth Regulators.” JOURNAL OF EXPERIMENTAL BOTANY 69.19 (2018): 4591–4607. Print.
APA
Nikonorova, N., Van den Broeck, L., Zhu, S., Van De Cotte, B., Dubois, M., Gevaert, K., Inzé, D., et al. (2018). Early mannitol-triggered changes in the Arabidopsis leaf (phospho) proteome reveal growth regulators. JOURNAL OF EXPERIMENTAL BOTANY, 69(19), 4591–4607.
Chicago author-date
Nikonorova, Natalia, Lisa Van den Broeck, Shanshuo Zhu, Brigitte Van De Cotte, Marieke Dubois, Kris Gevaert, Dirk Inzé, and Ive De Smet. 2018. “Early Mannitol-triggered Changes in the Arabidopsis Leaf (phospho) Proteome Reveal Growth Regulators.” Journal of Experimental Botany 69 (19): 4591–4607.
Chicago author-date (all authors)
Nikonorova, Natalia, Lisa Van den Broeck, Shanshuo Zhu, Brigitte Van De Cotte, Marieke Dubois, Kris Gevaert, Dirk Inzé, and Ive De Smet. 2018. “Early Mannitol-triggered Changes in the Arabidopsis Leaf (phospho) Proteome Reveal Growth Regulators.” Journal of Experimental Botany 69 (19): 4591–4607.
Vancouver
1.
Nikonorova N, Van den Broeck L, Zhu S, Van De Cotte B, Dubois M, Gevaert K, et al. Early mannitol-triggered changes in the Arabidopsis leaf (phospho) proteome reveal growth regulators. JOURNAL OF EXPERIMENTAL BOTANY. 2018;69(19):4591–607.
IEEE
[1]
N. Nikonorova et al., “Early mannitol-triggered changes in the Arabidopsis leaf (phospho) proteome reveal growth regulators,” JOURNAL OF EXPERIMENTAL BOTANY, vol. 69, no. 19, pp. 4591–4607, 2018.
@article{8575891,
  abstract     = {Leaf growth is a complex, quantitative trait, controlled by a plethora of regulatory mechanisms. Diverse environmental stimuli inhibit leaf growth to cope with the perceived stress. In plant research, mannitol is often used to impose osmotic stress and study the underlying growth-repressing mechanisms. In growing leaf tissue of plants briefly exposed to mannitol-induced stress, a highly interconnected gene regulatory network is induced. However, early signalling and associated protein phosphorylation events that probably precede part of these transcriptional changes and that potentially act at the onset of mannitol-induced leaf size reduction are largely unknown. Here, we performed a proteome and phosphoproteome analysis on growing leaf tissue of Arabidopsis thaliana plants exposed to mild mannitol-induced stress and captured the fast (within the first half hour) events associated with this stress. Based on this in-depth data analysis, 167 and 172 differentially regulated proteins and phosphorylated sites were found. We provide these data sets as a community resource and we flag differentially phosphorylated proteins with described growth-regulatory functions, but we also illustrate potential novel regulators of shoot growth.},
  author       = {Nikonorova, Natalia and Van den Broeck, Lisa and Zhu, Shanshuo and Van De Cotte, Brigitte and Dubois, Marieke and Gevaert, Kris and Inzé, Dirk and De Smet, Ive},
  issn         = {0022-0957},
  journal      = {JOURNAL OF EXPERIMENTAL BOTANY},
  keywords     = {MEMBRANE H+-ATPASE,SALT STRESS-RESPONSE,PLASMA-MEMBRANE,OSMOTIC-STRESS,PLANT DEVELOPMENT,ABIOTIC STRESSES,PHOSPHORYLATION,WATER,THALIANA,DROUGHT,AHA2,Arabidopsis thaliana,CRRSP38,leaf growth,mild osmotic stress,phosphoproteome,signalling},
  language     = {eng},
  number       = {19},
  pages        = {4591--4607},
  title        = {Early mannitol-triggered changes in the Arabidopsis leaf (phospho) proteome reveal growth regulators},
  url          = {http://dx.doi.org/10.1093/jxb/ery261},
  volume       = {69},
  year         = {2018},
}

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