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Comparative in vitro cytotoxicity of the emerging Fusarium mycotoxins beauvericin and enniatins to porcine intestinal epithelial cells

Sophie Fraeyman (UGent) , Evelyne Meyer (UGent) , Mathias Devreese (UGent) , Gunther Antonissen (UGent) , Kristel Demeyere (UGent) , Freddy Haesebrouck (UGent) and Siska Croubels (UGent)
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Abstract
The emerging Fusarium mycotoxins beauvericin (BEA) and enniatin (ENN) A, ENN A1, ENN B and ENN B1 gain increasing interest due to their highly prevalent contamination of cereals and cereal products. After oral intake, the gastro-intestinal tract is the first possible site of interaction. In the present in vitro study, the relative cytotoxicity of these mycotoxins towards proliferating and differentiated intestinal porcine epithelial cells of the jejunum (IPEC-J2) was evaluated using flow cytometric viability analysis. IPEC-J2 cells showed the highest sensitivity to BEA and ENN A. In proliferating cells, incubation for 24h with 10 mu M BEA caused complete disruption, while the viability percentage declined to 32% after 24h of incubation with 10 mu M ENN A. ENN A1 and ENN B1 were less cytotoxic with 87% and 93% viable cells after 24h of incubation with 10 mu M ENN A1 and B1, respectively. ENN B was the least cytotoxic since incubation at concentrations up to 100 mu M resulted in 83% viable proliferating cells. The same trend was observed for differentiated cells. The limited in vitro cytotoxic effect of ENN B on intestinal cells corroborates previous in vivo findings in broiler chicken in which dietary ENN B had minimal effect on intestinal morphometry.
Keywords
Enniatin A, Enniatin Al, Enniatin B, Enniatin B1, Beauvericin, Emerging, IPEC-J2, Apoptosis, Necrosis, Flow cytometry, MITOCHONDRIAL DAMAGE, CACO-2 CELLS, TOXINS, WHEAT, DEOXYNIVALENOL, TOXICITY, IONOPHORES, APOPTOSIS, CEREALS, GRAINS

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Chicago
Fraeyman, Sophie, Evelyne Meyer, Mathias Devreese, Gunther Antonissen, Kristel Demeyere, Freddy Haesebrouck, and Siska Croubels. 2018. “Comparative in Vitro Cytotoxicity of the Emerging Fusarium Mycotoxins Beauvericin and Enniatins to Porcine Intestinal Epithelial Cells.” Food and Chemical Toxicology 121: 566–572.
APA
Fraeyman, S., Meyer, E., Devreese, M., Antonissen, G., Demeyere, K., Haesebrouck, F., & Croubels, S. (2018). Comparative in vitro cytotoxicity of the emerging Fusarium mycotoxins beauvericin and enniatins to porcine intestinal epithelial cells. FOOD AND CHEMICAL TOXICOLOGY, 121, 566–572.
Vancouver
1.
Fraeyman S, Meyer E, Devreese M, Antonissen G, Demeyere K, Haesebrouck F, et al. Comparative in vitro cytotoxicity of the emerging Fusarium mycotoxins beauvericin and enniatins to porcine intestinal epithelial cells. FOOD AND CHEMICAL TOXICOLOGY. 2018;121:566–72.
MLA
Fraeyman, Sophie, Evelyne Meyer, Mathias Devreese, et al. “Comparative in Vitro Cytotoxicity of the Emerging Fusarium Mycotoxins Beauvericin and Enniatins to Porcine Intestinal Epithelial Cells.” FOOD AND CHEMICAL TOXICOLOGY 121 (2018): 566–572. Print.
@article{8575843,
  abstract     = {The emerging Fusarium mycotoxins beauvericin (BEA) and enniatin (ENN) A, ENN A1, ENN B and ENN B1 gain increasing interest due to their highly prevalent contamination of cereals and cereal products. After oral intake, the gastro-intestinal tract is the first possible site of interaction. In the present in vitro study, the relative cytotoxicity of these mycotoxins towards proliferating and differentiated intestinal porcine epithelial cells of the jejunum (IPEC-J2) was evaluated using flow cytometric viability analysis. IPEC-J2 cells showed the highest sensitivity to BEA and ENN A. In proliferating cells, incubation for 24h with 10 mu M BEA caused complete disruption, while the viability percentage declined to 32\% after 24h of incubation with 10 mu M ENN A. ENN A1 and ENN B1 were less cytotoxic with 87\% and 93\% viable cells after 24h of incubation with 10 mu M ENN A1 and B1, respectively. ENN B was the least cytotoxic since incubation at concentrations up to 100 mu M resulted in 83\% viable proliferating cells. The same trend was observed for differentiated cells. The limited in vitro cytotoxic effect of ENN B on intestinal cells corroborates previous in vivo findings in broiler chicken in which dietary ENN B had minimal effect on intestinal morphometry.},
  author       = {Fraeyman, Sophie and Meyer, Evelyne and Devreese, Mathias and Antonissen, Gunther and Demeyere, Kristel and Haesebrouck, Freddy and Croubels, Siska},
  issn         = {0278-6915},
  journal      = {FOOD AND CHEMICAL TOXICOLOGY},
  language     = {eng},
  pages        = {566--572},
  title        = {Comparative in vitro cytotoxicity of the emerging Fusarium mycotoxins beauvericin and enniatins to porcine intestinal epithelial cells},
  url          = {http://dx.doi.org/10.1016/j.fct.2018.09.053},
  volume       = {121},
  year         = {2018},
}

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