Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3
- Author
- Arvid Herrmann, Pantelis Livanos, Elisabeth Lipka, Astrid Gadeyne (UGent) , Marie-Theres Hauser, Daniël Van Damme (UGent) and Sabine Müller
- Organization
- Abstract
- Kinesins are versatile nano-machines that utilize variable non-motor domains to tune specific motor microtubule encounters. During plant cytokinesis, the kinesin-12 orthologs, PHRAGMO-PLAST ORIENTING KINESIN (POK)1 and POK2, are essential for rapid centrifugal expansion of the cytokinetic apparatus, the phragmoplast, toward a pre-selected cell plate fusion site at the cell cortex. Here, we report on the spatio-temporal localization pattern of POK2, mediated by distinct protein domains. Functional dissection of POK2 domains revealed the association of POK2 with the site of the future cell division plane and with the phragmoplast during cytokinesis. Accumulation of POK2 at the phragmoplast midzone depends on its functional POK2 motor domain and is fine-tuned by its carboxy-terminal region that also directs POK2 to the division site. Furthermore, POK2 likely stabilizes the phragmoplast midzone via interaction with the conserved microtubule-associated protein MAP65-3/PLEIADE, a well-established microtubule cross-linker. Collectively, our results suggest that dual localized POK2 plays multiple roles during plant cell division.
- Keywords
- ARABIDOPSIS-THALIANA, PLANT CYTOKINESIS, ANTIPARALLEL MICROTUBULES, PREPROPHASE BAND, PROTEIN MAP65-3, MAP KINASE, PHRAGMOPLAST, MEMBRANE, BINDING, PROGRESSION, cytokinesis, cytoskeleton, division plane, phragmoplast, preprophase band
Downloads
-
(...).pdf
- full text
- |
- UGent only
- |
- |
- 3.62 MB
Citation
Please use this url to cite or link to this publication: http://hdl.handle.net/1854/LU-8575756
- MLA
- Herrmann, Arvid, et al. “Dual Localized Kinesin-12 POK2 Plays Multiple Roles during Cell Division and Interacts with MAP65-3.” EMBO REPORTS, vol. 19, no. 9, 2018, doi:10.15252/embr.201846085.
- APA
- Herrmann, A., Livanos, P., Lipka, E., Gadeyne, A., Hauser, M.-T., Van Damme, D., & Müller, S. (2018). Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3. EMBO REPORTS, 19(9). https://doi.org/10.15252/embr.201846085
- Chicago author-date
- Herrmann, Arvid, Pantelis Livanos, Elisabeth Lipka, Astrid Gadeyne, Marie-Theres Hauser, Daniël Van Damme, and Sabine Müller. 2018. “Dual Localized Kinesin-12 POK2 Plays Multiple Roles during Cell Division and Interacts with MAP65-3.” EMBO REPORTS 19 (9). https://doi.org/10.15252/embr.201846085.
- Chicago author-date (all authors)
- Herrmann, Arvid, Pantelis Livanos, Elisabeth Lipka, Astrid Gadeyne, Marie-Theres Hauser, Daniël Van Damme, and Sabine Müller. 2018. “Dual Localized Kinesin-12 POK2 Plays Multiple Roles during Cell Division and Interacts with MAP65-3.” EMBO REPORTS 19 (9). doi:10.15252/embr.201846085.
- Vancouver
- 1.Herrmann A, Livanos P, Lipka E, Gadeyne A, Hauser M-T, Van Damme D, et al. Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3. EMBO REPORTS. 2018;19(9).
- IEEE
- [1]A. Herrmann et al., “Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3,” EMBO REPORTS, vol. 19, no. 9, 2018.
@article{8575756, abstract = {{Kinesins are versatile nano-machines that utilize variable non-motor domains to tune specific motor microtubule encounters. During plant cytokinesis, the kinesin-12 orthologs, PHRAGMO-PLAST ORIENTING KINESIN (POK)1 and POK2, are essential for rapid centrifugal expansion of the cytokinetic apparatus, the phragmoplast, toward a pre-selected cell plate fusion site at the cell cortex. Here, we report on the spatio-temporal localization pattern of POK2, mediated by distinct protein domains. Functional dissection of POK2 domains revealed the association of POK2 with the site of the future cell division plane and with the phragmoplast during cytokinesis. Accumulation of POK2 at the phragmoplast midzone depends on its functional POK2 motor domain and is fine-tuned by its carboxy-terminal region that also directs POK2 to the division site. Furthermore, POK2 likely stabilizes the phragmoplast midzone via interaction with the conserved microtubule-associated protein MAP65-3/PLEIADE, a well-established microtubule cross-linker. Collectively, our results suggest that dual localized POK2 plays multiple roles during plant cell division.}}, articleno = {{e46085}}, author = {{Herrmann, Arvid and Livanos, Pantelis and Lipka, Elisabeth and Gadeyne, Astrid and Hauser, Marie-Theres and Van Damme, Daniël and Müller, Sabine}}, issn = {{1469-221X}}, journal = {{EMBO REPORTS}}, keywords = {{ARABIDOPSIS-THALIANA,PLANT CYTOKINESIS,ANTIPARALLEL MICROTUBULES,PREPROPHASE BAND,PROTEIN MAP65-3,MAP KINASE,PHRAGMOPLAST,MEMBRANE,BINDING,PROGRESSION,cytokinesis,cytoskeleton,division plane,phragmoplast,preprophase band}}, language = {{eng}}, number = {{9}}, pages = {{16}}, title = {{Dual localized kinesin-12 POK2 plays multiple roles during cell division and interacts with MAP65-3}}, url = {{http://doi.org/10.15252/embr.201846085}}, volume = {{19}}, year = {{2018}}, }
- Altmetric
- View in Altmetric
- Web of Science
- Times cited: