Advanced search
1 file | 2.67 MB

Arabidopsis leaf flatness is regulated by PPD2 and NINJA through repression of CYCLIN D3 genes

Alexandra Baekelandt (UGent) , Laurens Pauwels (UGent) , Zhibiao Wang, Na Li, Liesbeth De Milde (UGent) , Annelore Natran (UGent) , Mattias Vermeersch (UGent) , Yunhai Li, Alain Goossens (UGent) , Dirk Inzé (UGent) , et al.
(2018) PLANT PHYSIOLOGY. 178(1). p.217-232
Author
Organization
Abstract
In Arabidopsis (Arabidopsis thaliana), reduced expression of the transcriptional regulator PEAPOD2 (PPD2) results in propeller-like rosettes with enlarged and dome-shaped leaves. However, the molecular and cellular processes underlying this peculiar phenotype remain elusive. Here, we studied the interaction between PPD2 and NOVEL INTERACTOR OF JAZ (NINJA) and demonstrated that ninja loss-of-function plants produce rosettes with dome-shaped leaves similar to those of ppd mutants but without the increase in size. We showed that ninja mutants have a convex-shaped primary cell cycle arrest front, putatively leading to excessive cell division in the central leaf blade region. Furthermore, ppd and ninja mutants have a similar increase in the expression of CYCLIN D3;2 (CYCD3;2), and ectopic overexpression of CYCD3;2 phenocopies the ppd and ninja rosette and leaf shape phenotypes without affecting the size. Our results reveal a pivotal contribution of NINJA in leaf development, in addition to its well-studied function in jasmonate signaling, and imply a new function for D3-type cyclins in, at least partially, uncoupling the size and shape phenotypes of ppd leaves.
Keywords
STOMATAL DEVELOPMENT, CELL-CYCLE, SURFACE CURVATURE, DEVELOPING LEAVES, DEPENDENT KINASE, THALIANA, JASMONATE, GROWTH, PROLIFERATION, DIVISION

Downloads

  • Baeckelandt et al. (2018) Plant Physiology 178,217.pdf
    • full text
    • |
    • open access
    • |
    • PDF
    • |
    • 2.67 MB

Citation

Please use this url to cite or link to this publication:

Chicago
Baekelandt, Alexandra, Laurens Pauwels, Zhibiao Wang, Na Li, Liesbeth De Milde, Annelore Natran, Mattias Vermeersch, et al. 2018. “Arabidopsis Leaf Flatness Is Regulated by PPD2 and NINJA Through Repression of CYCLIN D3 Genes.” Plant Physiology 178 (1): 217–232.
APA
Baekelandt, A., Pauwels, L., Wang, Z., Li, N., De Milde, L., Natran, A., Vermeersch, M., et al. (2018). Arabidopsis leaf flatness is regulated by PPD2 and NINJA through repression of CYCLIN D3 genes. PLANT PHYSIOLOGY, 178(1), 217–232.
Vancouver
1.
Baekelandt A, Pauwels L, Wang Z, Li N, De Milde L, Natran A, et al. Arabidopsis leaf flatness is regulated by PPD2 and NINJA through repression of CYCLIN D3 genes. PLANT PHYSIOLOGY. 2018;178(1):217–32.
MLA
Baekelandt, Alexandra, Laurens Pauwels, Zhibiao Wang, et al. “Arabidopsis Leaf Flatness Is Regulated by PPD2 and NINJA Through Repression of CYCLIN D3 Genes.” PLANT PHYSIOLOGY 178.1 (2018): 217–232. Print.
@article{8575745,
  abstract     = {In Arabidopsis (Arabidopsis thaliana), reduced expression of the transcriptional regulator PEAPOD2 (PPD2) results in propeller-like rosettes with enlarged and dome-shaped leaves. However, the molecular and cellular processes underlying this peculiar phenotype remain elusive. Here, we studied the interaction between PPD2 and NOVEL INTERACTOR OF JAZ (NINJA) and demonstrated that ninja loss-of-function plants produce rosettes with dome-shaped leaves similar to those of ppd mutants but without the increase in size. We showed that ninja mutants have a convex-shaped primary cell cycle arrest front, putatively leading to excessive cell division in the central leaf blade region. Furthermore, ppd and ninja mutants have a similar increase in the expression of CYCLIN D3;2 (CYCD3;2), and ectopic overexpression of CYCD3;2 phenocopies the ppd and ninja rosette and leaf shape phenotypes without affecting the size. Our results reveal a pivotal contribution of NINJA in leaf development, in addition to its well-studied function in jasmonate signaling, and imply a new function for D3-type cyclins in, at least partially, uncoupling the size and shape phenotypes of ppd leaves.},
  author       = {Baekelandt, Alexandra and Pauwels, Laurens and Wang, Zhibiao and Li, Na and De Milde, Liesbeth and Natran, Annelore and Vermeersch, Mattias and Li, Yunhai and Goossens, Alain and Inz{\'e}, Dirk and Gonzalez Sanchez, Nathalie},
  issn         = {0032-0889},
  journal      = {PLANT PHYSIOLOGY},
  language     = {eng},
  number       = {1},
  pages        = {217--232},
  title        = {Arabidopsis leaf flatness is regulated by PPD2 and NINJA through repression of CYCLIN D3 genes},
  url          = {http://dx.doi.org/10.1104/pp.18.00327},
  volume       = {178},
  year         = {2018},
}

Altmetric
View in Altmetric
Web of Science
Times cited: