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Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport

(2018) PLANT GROWTH REGULATION. 86(1). p.23-36
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Abstract
Shoot branching is essential in ornamental chrysanthemum production and determines final plant shape and quality. Auxin is associated with apical dominance to indirectly inhibit bud outgrowth. Two non-mutually exclusive models exist for indirect auxin inhibition. Basipetal auxin transport inhibits axillary bud outgrowth by limiting auxin export from buds to stem (canalization model) or by increasing strigolactone levels (second messenger model). Here we analyzed bud outgrowth in treatments with auxin (IAA), strigolactone (GR24) and auxin transport inhibitor (NPA) using a split-plate bioassay with isolated chrysanthemum stem segments. Besides measuring bud length, dividing cell percentage was measured with flow cytometry and RT-qPCR was used to monitor expression levels of genes involved in auxin transport (CmPIN1) and signaling (CmAXR2), bud dormancy (CmBRC1, CmDRM1) and strigolactone biosynthesis (CmMAX1, CmMAX3). Treatments over a 5-day period showed bud outgrowth in the control and inhibition with IAA and IAA + GR24. Bud outgrowth in the control coincided with high dividing cell percentage, decreased expression of CmBRC1 and CmDRM1 and increased CmPIN1 expression. Inhibition by IAA and IAA + GR24 coincided with low dividing cell percentage and unchanged or increased expressions of CmBRC1, CmDRM1 and CmPIN1. Treatment with GR24 showed restricted bud outgrowth that was counteracted by NPA. This restricted bud outgrowth was still concomitant with a high dividing cell percentage and coincided with decreased expression of dormancy genes. These results indicate incomplete inhibition of bud outgrowth by GR24 treatment and suggest involvement of auxin transport in the mechanism of bud inhibition by strigolactones, supporting the canalization model.
Keywords
Shoot branching, Chrysanthemum, Axillary bud outgrowth, Auxin transport, Strigolactone, Gene expression, Flow cytometry, Split-plate, Dormancy, DENDRANTHEMA-GRANDIFLORUM, FEEDBACK-REGULATION, PLANT DEVELOPMENT, CELL-CYCLE, ARABIDOPSIS, INHIBITION, FLOW, PEA, CANALIZATION, DOWNSTREAM

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Citation

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MLA
Dierck, Robrecht, Leen Leus, Emmy Dhooghe, et al. “Branching Gene Expression During Chrysanthemum Axillary Bud Outgrowth Regulated by Strigolactone and Auxin Transport.” PLANT GROWTH REGULATION 86.1 (2018): 23–36. Print.
APA
Dierck, Robrecht, Leus, L., Dhooghe, E., Van Huylenbroeck, J., De Riek, J., Van Der Straeten, D., & De Keyser, E. (2018). Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport. PLANT GROWTH REGULATION, 86(1), 23–36.
Chicago author-date
Dierck, Robrecht, Leen Leus, Emmy Dhooghe, Johan Van Huylenbroeck, Jan De Riek, Dominique Van Der Straeten, and Ellen De Keyser. 2018. “Branching Gene Expression During Chrysanthemum Axillary Bud Outgrowth Regulated by Strigolactone and Auxin Transport.” Plant Growth Regulation 86 (1): 23–36.
Chicago author-date (all authors)
Dierck, Robrecht, Leen Leus, Emmy Dhooghe, Johan Van Huylenbroeck, Jan De Riek, Dominique Van Der Straeten, and Ellen De Keyser. 2018. “Branching Gene Expression During Chrysanthemum Axillary Bud Outgrowth Regulated by Strigolactone and Auxin Transport.” Plant Growth Regulation 86 (1): 23–36.
Vancouver
1.
Dierck R, Leus L, Dhooghe E, Van Huylenbroeck J, De Riek J, Van Der Straeten D, et al. Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport. PLANT GROWTH REGULATION. 2018;86(1):23–36.
IEEE
[1]
R. Dierck et al., “Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport,” PLANT GROWTH REGULATION, vol. 86, no. 1, pp. 23–36, 2018.
@article{8574452,
  abstract     = {Shoot branching is essential in ornamental chrysanthemum production and determines final plant shape and quality. Auxin is associated with apical dominance to indirectly inhibit bud outgrowth. Two non-mutually exclusive models exist for indirect auxin inhibition. Basipetal auxin transport inhibits axillary bud outgrowth by limiting auxin export from buds to stem (canalization model) or by increasing strigolactone levels (second messenger model). Here we analyzed bud outgrowth in treatments with auxin (IAA), strigolactone (GR24) and auxin transport inhibitor (NPA) using a split-plate bioassay with isolated chrysanthemum stem segments. Besides measuring bud length, dividing cell percentage was measured with flow cytometry and RT-qPCR was used to monitor expression levels of genes involved in auxin transport (CmPIN1) and signaling (CmAXR2), bud dormancy (CmBRC1, CmDRM1) and strigolactone biosynthesis (CmMAX1, CmMAX3). Treatments over a 5-day period showed bud outgrowth in the control and inhibition with IAA and IAA + GR24. Bud outgrowth in the control coincided with high dividing cell percentage, decreased expression of CmBRC1 and CmDRM1 and increased CmPIN1 expression. Inhibition by IAA and IAA + GR24 coincided with low dividing cell percentage and unchanged or increased expressions of CmBRC1, CmDRM1 and CmPIN1. Treatment with GR24 showed restricted bud outgrowth that was counteracted by NPA. This restricted bud outgrowth was still concomitant with a high dividing cell percentage and coincided with decreased expression of dormancy genes. These results indicate incomplete inhibition of bud outgrowth by GR24 treatment and suggest involvement of auxin transport in the mechanism of bud inhibition by strigolactones, supporting the canalization model.},
  author       = {Dierck, Robrecht and Leus, Leen and Dhooghe, Emmy and Van Huylenbroeck, Johan and De Riek, Jan and Van Der Straeten, Dominique and De Keyser, Ellen},
  issn         = {0167-6903},
  journal      = {PLANT GROWTH REGULATION},
  keywords     = {Shoot branching,Chrysanthemum,Axillary bud outgrowth,Auxin transport,Strigolactone,Gene expression,Flow cytometry,Split-plate,Dormancy,DENDRANTHEMA-GRANDIFLORUM,FEEDBACK-REGULATION,PLANT DEVELOPMENT,CELL-CYCLE,ARABIDOPSIS,INHIBITION,FLOW,PEA,CANALIZATION,DOWNSTREAM},
  language     = {eng},
  number       = {1},
  pages        = {23--36},
  title        = {Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport},
  url          = {http://dx.doi.org/10.1007/s10725-018-0408-2},
  volume       = {86},
  year         = {2018},
}

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