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The evaluation of the transport medium for extracted premolars prior to cryopreservation : an in vitro study

Noëmi De Roo (UGent) , Laurent Thierens (UGent) , Liesbeth Temmerman (UGent) , Elise Dekeyser (UGent) , Jerina Boelens (UGent) and Guy De Pauw (UGent)
(2018) CELL AND TISSUE BANKING. 19(4). p.697-704
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Abstract
Autotransplantation is a versatile technique for the replacement of a missing tooth and cryopreservation can expand its scope. The aim of this in vitro study is to compare the antimicrobial effect of different transport protocols on procured teeth prior to cryopreservation. Streptococcus oralis biofilms were grown on ten sterile premolars, incubated for 48h and subjected to the following transport procedures: an untreated (contaminated) control group, a group rinsed with phosphate buffered saline (PBS), a group transported in PBS, a group transported in Dulbecco's Modified Eagle's medium (DMEM) supplemented with fetal calf serum (FCS), and a group transported in DMEM supplemented with FCS and antibiotics (AB). The effect of cryopreservation as such, as well as the combination with a transport medium (DMEM+FCS+AB) on the contamination was also tested. The surviving bacteria were harvested, and determined by plate counting. There was no significant reduction in contamination after rinsing the tooth, after transport in PBS or after transport in DMEM with FCS. Significant reductions were observed for transport in DMEM with AB when compared to the control group (p=0.003). Cryopreservation as such reduced the biofilm significantly (p<0.001). No cumulative effect could be found when transport in DMEM+FCS+AB was followed by cryopreservation. Within the limitations of this laboratory set-up, DMEM+FCS+AB was the most effective transport medium in S. oralis biofilm elimination. It could not be concluded that rinsing of the tooth gives an additional reduction. Cryopreservation as such decontaminated the teeth more effectively than any tested transport procedure.
Keywords
Autotransplantation, Cryopreservation, Transport, Tissue culture media, Antibiotics, Decontamination, Tooth bank, BACTERIAL DIVERSITY, CONTAMINATION

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MLA
De Roo, Noëmi et al. “The Evaluation of the Transport Medium for Extracted Premolars Prior to Cryopreservation : an in Vitro Study.” CELL AND TISSUE BANKING 19.4 (2018): 697–704. Print.
APA
De Roo, N., Thierens, L., Temmerman, L., Dekeyser, E., Boelens, J., & De Pauw, G. (2018). The evaluation of the transport medium for extracted premolars prior to cryopreservation : an in vitro study. CELL AND TISSUE BANKING, 19(4), 697–704.
Chicago author-date
De Roo, Noëmi, Laurent Thierens, Liesbeth Temmerman, Elise Dekeyser, Jerina Boelens, and Guy De Pauw. 2018. “The Evaluation of the Transport Medium for Extracted Premolars Prior to Cryopreservation : an in Vitro Study.” Cell and Tissue Banking 19 (4): 697–704.
Chicago author-date (all authors)
De Roo, Noëmi, Laurent Thierens, Liesbeth Temmerman, Elise Dekeyser, Jerina Boelens, and Guy De Pauw. 2018. “The Evaluation of the Transport Medium for Extracted Premolars Prior to Cryopreservation : an in Vitro Study.” Cell and Tissue Banking 19 (4): 697–704.
Vancouver
1.
De Roo N, Thierens L, Temmerman L, Dekeyser E, Boelens J, De Pauw G. The evaluation of the transport medium for extracted premolars prior to cryopreservation : an in vitro study. CELL AND TISSUE BANKING. 2018;19(4):697–704.
IEEE
[1]
N. De Roo, L. Thierens, L. Temmerman, E. Dekeyser, J. Boelens, and G. De Pauw, “The evaluation of the transport medium for extracted premolars prior to cryopreservation : an in vitro study,” CELL AND TISSUE BANKING, vol. 19, no. 4, pp. 697–704, 2018.
@article{8573416,
  abstract     = {Autotransplantation is a versatile technique for the replacement of a missing tooth and cryopreservation can expand its scope. The aim of this in vitro study is to compare the antimicrobial effect of different transport protocols on procured teeth prior to cryopreservation. Streptococcus oralis biofilms were grown on ten sterile premolars, incubated for 48h and subjected to the following transport procedures: an untreated (contaminated) control group, a group rinsed with phosphate buffered saline (PBS), a group transported in PBS, a group transported in Dulbecco's Modified Eagle's medium (DMEM) supplemented with fetal calf serum (FCS), and a group transported in DMEM supplemented with FCS and antibiotics (AB). The effect of cryopreservation as such, as well as the combination with a transport medium (DMEM+FCS+AB) on the contamination was also tested. The surviving bacteria were harvested, and determined by plate counting. There was no significant reduction in contamination after rinsing the tooth, after transport in PBS or after transport in DMEM with FCS. Significant reductions were observed for transport in DMEM with AB when compared to the control group (p=0.003). Cryopreservation as such reduced the biofilm significantly (p<0.001). No cumulative effect could be found when transport in DMEM+FCS+AB was followed by cryopreservation. Within the limitations of this laboratory set-up, DMEM+FCS+AB was the most effective transport medium in S. oralis biofilm elimination. It could not be concluded that rinsing of the tooth gives an additional reduction. Cryopreservation as such decontaminated the teeth more effectively than any tested transport procedure.},
  author       = {De Roo, Noëmi and Thierens, Laurent and Temmerman, Liesbeth and Dekeyser, Elise and Boelens, Jerina and De Pauw, Guy},
  issn         = {1389-9333},
  journal      = {CELL AND TISSUE BANKING},
  keywords     = {Autotransplantation,Cryopreservation,Transport,Tissue culture media,Antibiotics,Decontamination,Tooth bank,BACTERIAL DIVERSITY,CONTAMINATION},
  language     = {eng},
  number       = {4},
  pages        = {697--704},
  title        = {The evaluation of the transport medium for extracted premolars prior to cryopreservation : an in vitro study},
  url          = {http://dx.doi.org/10.1007/s10561-018-9722-1},
  volume       = {19},
  year         = {2018},
}

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