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Development of a nanobody-based lateral flow assay to detect active Trypanosoma congolense infections

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Abstract
Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as 'test-of-cure' tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.
Keywords
MEDIATED ISOTHERMAL AMPLIFICATION, ANIMAL AFRICAN TRYPANOSOMOSIS, SINGLE-DOMAIN ANTIBODIES, EXTRACELLULAR VESICLES, GLYCOLYTIC-ENZYMES, MOLECULAR SURVEY, NORTHERN TOGO, CATTLE, DIAGNOSIS, ANTIGEN

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MLA
Pinto Torres, Joar E, Julie Goossens, Jianzu Ding, et al. “Development of a Nanobody-based Lateral Flow Assay to Detect Active Trypanosoma Congolense Infections.” SCIENTIFIC REPORTS 8 (2018): n. pag. Print.
APA
Pinto Torres, J. E., Goossens, J., Ding, J., Li, Z., Lu, S., Vertommen, D., Naniima, P., et al. (2018). Development of a nanobody-based lateral flow assay to detect active Trypanosoma congolense infections. SCIENTIFIC REPORTS, 8.
Chicago author-date
Pinto Torres, Joar E, Julie Goossens, Jianzu Ding, Zeng Li, Shaohong Lu, Didier Vertommen, Peter Naniima, et al. 2018. “Development of a Nanobody-based Lateral Flow Assay to Detect Active Trypanosoma Congolense Infections.” Scientific Reports 8.
Chicago author-date (all authors)
Pinto Torres, Joar E, Julie Goossens, Jianzu Ding, Zeng Li, Shaohong Lu, Didier Vertommen, Peter Naniima, Rui Chen, Serge Muyldermans, Yann G-J Sterckx, and Stefan Magez. 2018. “Development of a Nanobody-based Lateral Flow Assay to Detect Active Trypanosoma Congolense Infections.” Scientific Reports 8.
Vancouver
1.
Pinto Torres JE, Goossens J, Ding J, Li Z, Lu S, Vertommen D, et al. Development of a nanobody-based lateral flow assay to detect active Trypanosoma congolense infections. SCIENTIFIC REPORTS. 2018;8.
IEEE
[1]
J. E. Pinto Torres et al., “Development of a nanobody-based lateral flow assay to detect active Trypanosoma congolense infections,” SCIENTIFIC REPORTS, vol. 8, 2018.
@article{8572169,
  abstract     = {Animal African trypanosomosis (AAT), a disease affecting livestock, is caused by parasites of the Trypanosoma genus (mainly T. vivax and T. congolense). AAT is widespread in Sub-Saharan Africa, where it continues to impose a heavy socio-economic burden as it renders development of sustainable livestock rearing very strenuous. Active case-finding and the identification of infected animals prior to initiation of drug treatment requires the availability of sensitive and specific diagnostic tests. In this paper, we describe the development of two heterologous sandwich assay formats (ELISA and LFA) for T. congolense detection through the use of Nanobodies (Nbs). The immunisation of an alpaca with a secretome mix from two T. congolense strains resulted in the identification of a Nb pair (Nb44/Nb42) that specifically targets the glycolytic enzyme pyruvate kinase. We demonstrate that the Nb44/Nb42 ELISA and LFA can be employed to detect parasitaemia in plasma samples from experimentally infected mice and cattle and, additionally, that they can serve as 'test-of-cure' tools. Altogether, the findings in this paper present the development and evaluation of the first Nb-based antigen detection LFA to identify active T. congolense infections.},
  articleno    = {9019},
  author       = {Pinto Torres, Joar E and Goossens, Julie and Ding, Jianzu and Li, Zeng and Lu, Shaohong and Vertommen, Didier and Naniima, Peter and Chen, Rui and Muyldermans, Serge and Sterckx, Yann G-J and Magez, Stefan},
  issn         = {2045-2322},
  journal      = {SCIENTIFIC REPORTS},
  keywords     = {MEDIATED ISOTHERMAL AMPLIFICATION,ANIMAL AFRICAN TRYPANOSOMOSIS,SINGLE-DOMAIN ANTIBODIES,EXTRACELLULAR VESICLES,GLYCOLYTIC-ENZYMES,MOLECULAR SURVEY,NORTHERN TOGO,CATTLE,DIAGNOSIS,ANTIGEN},
  language     = {eng},
  pages        = {15},
  title        = {Development of a nanobody-based lateral flow assay to detect active Trypanosoma congolense infections},
  url          = {http://dx.doi.org/10.1038/s41598-018-26732-7},
  volume       = {8},
  year         = {2018},
}

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